Overview

  • Product name
    Anti-COX2 / Cyclooxygenase 2 antibody
    See all COX2 / Cyclooxygenase 2 primary antibodies
  • Description
    Rabbit polyclonal to COX2 / Cyclooxygenase 2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ELISA, WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human COX2/ Cyclooxygenase 2.

  • Positive control
    • Extracts from A549 cells. This antibody gave a positive result in IHC in the following FFPE tissue: Human lung adenocarcinoma.

Applications

Our Abpromise guarantee covers the use of ab52237 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/20000.
WB 1/500 - 1/1000. Detects a band of approximately 69 kDa (predicted molecular weight: 69 kDa).
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/500.

Target

  • Function
    Mediates the formation of prostaglandins from arachidonate. May have a role as a major mediator of inflammation and/or a role for prostanoid signaling in activity-dependent plasticity.
  • Pathway
    Lipid metabolism; prostaglandin biosynthesis.
  • Sequence similarities
    Belongs to the prostaglandin G/H synthase family.
    Contains 1 EGF-like domain.
  • Post-translational
    modifications
    S-nitrosylation by NOS2 (iNOS) activates enzme activity. S-nitrosylation may take place on different Cys residues in addition to Cys-561.
  • Cellular localization
    Microsome membrane. Endoplasmic reticulum membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • COX 2 antibody
    • COX-2 antibody
    • COX2 antibody
    • Cyclooxygenase 2 antibody
    • Cyclooxygenase 2b antibody
    • Cyclooxygenase antibody
    • Cyclooxygenase-2 antibody
    • Cyclooxygenase2 antibody
    • EC 1.14.99.1 antibody
    • fj02a10 antibody
    • Glucocorticoid-regulated inflammatory cyclooxygenase antibody
    • Glucocorticoid-regulated inflammatory Prostaglandin G/H synthase antibody
    • GRIPGHS antibody
    • hCox 2 antibody
    • Macrophage activation-associated marker protein P71/73 antibody
    • OTTHUMP00000033524 antibody
    • PES-2 antibody
    • PGG/HS antibody
    • PGH synthase 2 antibody
    • PGH2_HUMAN antibody
    • PGHS 2 antibody
    • PGHS-2 antibody
    • PGHS2 antibody
    • PHS 2 antibody
    • PHS II antibody
    • PHS2 antibody
    • Prostaglandin endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase) antibody
    • Prostaglandin endoperoxide synthase 2 antibody
    • Prostaglandin G/H synthase 2 antibody
    • Prostaglandin G/H synthase 2 precursor antibody
    • Prostaglandin G/H synthase and cyclooxygenase antibody
    • Prostaglandin G/H synthase antibody
    • Prostaglandin H2 synthase 2 antibody
    • prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase) antibody
    • Prostaglandin-endoperoxide synthase 2 antibody
    • PTGS2 antibody
    • ptgs2a antibody
    • TIS10 antibody
    • TIS10 protein antibody
    • unp1239 antibody
    • wu:fj02a10 antibody
    see all

Images

  • Anti-COX2 / Cyclooxygenase 2 antibody (ab52237) at 1/1000 dilution + Human primary myometrial cells whole cell lysate at 20 µg

    Secondary
    Anti-rabbit IgG, HRP-linked at 1/4000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 69 kDa
    Observed band size: 70 kDa
    why is the actual band size different from the predicted?


    Exposure time: 1 minute

    See Abreview

  • IHC image of COX2 / Cyclooxygenase 2 staining in Human lung adenocarcinoma formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab52237, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • All lanes : Anti-COX2 / Cyclooxygenase 2 antibody (ab52237) at 1/500 dilution

    Lane 1 : A549 (Human lung adenocarcinoma epithelial)
    Lane 2 : A549 (Human lung adenocarcinoma epithelial) with Synthesized Peptide

    Predicted band size: 69 kDa

  • Immunocytochemistry/immunofluorescence analysis of A549 (Human lung adenocarcinoma epithelial) cells labelling COX2 / Cyclooxygenase 2 with ab52237 at dilution of 1/100. No staining was abserved when blocked with synthesized peptide as shown in the inset.

  • Anti-COX2 / Cyclooxygenase 2 antibody (ab52237) (Human mesothelioma whole cell lysate.)
    Secondary
    Goat anti-rabbit HRP

    Predicted band size: 69 kDa



    Please see accompanying abreview for additional information

    See Abreview

References

This product has been referenced in:
  • Wang M  et al. Effect of cyclooxygenase-2 inhibition on the development of post-traumatic stress disorder in rats. Mol Med Rep 17:4925-4932 (2018). Read more (PubMed: 29393449) »
  • Zhang P  et al. Glial interleukin-1ß upregulates neuronal sodium channel 1.7 in trigeminal ganglion contributing to temporomandibular joint inflammatory hypernociception in rats. J Neuroinflammation 15:117 (2018). Read more (PubMed: 29678208) »
See all 32 Publications for this product

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Tissue lysate - whole (Pooled endometrial tissue)
Gel Running Conditions
Reduced Denaturing (12%)
Loading amount
20 µg
Specification
Pooled endometrial tissue
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Jun 30 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (primary myometrial cells)
Loading amount
20 µg
Specification
primary myometrial cells
Treatment
not serum starved
Gel Running Conditions
Reduced Denaturing (8% Bis-Tris)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Oct 09 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rat Tissue lysate - whole (Lung)
Loading amount
30 µg
Specification
Lung
Gel Running Conditions
Reduced Denaturing (12%)
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C

Mr. Weibin Fang

Verified customer

Submitted Feb 10 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (epithelial)
Loading amount
25 µg
Specification
epithelial
Gel Running Conditions
Non-reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Aug 05 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Mesothelioma)
Loading amount
20 µg
Specification
Mesothelioma
Gel Running Conditions
Non-reduced Denaturing (12% Tris-HEPES-SDS)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

Abcam user community

Verified customer

Submitted Mar 06 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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