• Product name
    Anti-COX2 / Cyclooxygenase 2 antibody
    See all COX2 / Cyclooxygenase 2 primary antibodies
  • Description
    Rabbit polyclonal to COX2 / Cyclooxygenase 2
  • Host species
  • Specificity
    The antibody does not cross react with Cox-1.
  • Tested applications
    Suitable for: WB, ELISA, Dot blotmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fusion protein (Human C-terminal end)

  • General notes
    COX-2, also known as prostaglandin H synthase, was identified less than a decade ago. Its discovery was followed by a period of discovery and drug development to create a “super aspirin” and led to insights into arthritis, Alzheimer's disease, colorectal cancer and regulation of brain and kidney function in search of better treatments for degenerative and inflammatory diseases. Two different isoforms of the enzyme are known, Cox-1 and Cox-2.


Our Abpromise guarantee covers the use of ab6665 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Dot blot
  • Application notes
    Cox-2 (Cyclooxygenase-2) is an inducible enzyme that is normally absent from cells, however, in response to growth factors, tumor promoters and some cytokines, it undergoes a rapid and transient expression.
    This antiserum against Human Cox-2 and has been tested for use in immunoblotting (1:5,000 to 1:10,000) and ELISA (1:10,000 to 1:50,000).
    The antibody recognizes Cox-2 at 70kDa in Sf9 cell lines transfected with Cox-2 as well as in WISH cells induced with IL-1b.
  • Target

    • Function
      Mediates the formation of prostaglandins from arachidonate. May have a role as a major mediator of inflammation and/or a role for prostanoid signaling in activity-dependent plasticity.
    • Pathway
      Lipid metabolism; prostaglandin biosynthesis.
    • Sequence similarities
      Belongs to the prostaglandin G/H synthase family.
      Contains 1 EGF-like domain.
    • Post-translational
      S-nitrosylation by NOS2 (iNOS) activates enzme activity. S-nitrosylation may take place on different Cys residues in addition to Cys-561.
    • Cellular localization
      Microsome membrane. Endoplasmic reticulum membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • COX 2 antibody
      • COX-2 antibody
      • COX2 antibody
      • Cyclooxygenase 2 antibody
      • Cyclooxygenase 2b antibody
      • Cyclooxygenase antibody
      • Cyclooxygenase-2 antibody
      • Cyclooxygenase2 antibody
      • EC antibody
      • fj02a10 antibody
      • Glucocorticoid-regulated inflammatory cyclooxygenase antibody
      • Glucocorticoid-regulated inflammatory Prostaglandin G/H synthase antibody
      • GRIPGHS antibody
      • hCox 2 antibody
      • Macrophage activation-associated marker protein P71/73 antibody
      • OTTHUMP00000033524 antibody
      • PES-2 antibody
      • PGG/HS antibody
      • PGH synthase 2 antibody
      • PGH2_HUMAN antibody
      • PGHS 2 antibody
      • PGHS-2 antibody
      • PGHS2 antibody
      • PHS 2 antibody
      • PHS II antibody
      • PHS2 antibody
      • Prostaglandin endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase) antibody
      • Prostaglandin endoperoxide synthase 2 antibody
      • Prostaglandin G/H synthase 2 antibody
      • Prostaglandin G/H synthase 2 precursor antibody
      • Prostaglandin G/H synthase and cyclooxygenase antibody
      • Prostaglandin G/H synthase antibody
      • Prostaglandin H2 synthase 2 antibody
      • prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase) antibody
      • Prostaglandin-endoperoxide synthase 2 antibody
      • PTGS2 antibody
      • ptgs2a antibody
      • TIS10 antibody
      • TIS10 protein antibody
      • unp1239 antibody
      • wu:fj02a10 antibody
      see all


    This product has been referenced in:
    • Li Q  et al. Inhibition of neuronal ferroptosis protects hemorrhagic brain. JCI Insight 2:e90777 (2017). Read more (PubMed: 28405617) »
    • Hayashi G  et al. Frataxin deficiency increases cyclooxygenase 2 and prostaglandins in cell and animal models of Friedreich's ataxia. Hum Mol Genet N/A:N/A (2014). WB ; Mouse . Read more (PubMed: 25104852) »

    See all 14 Publications for this product

    Customer reviews and Q&As

    Thank you for your enquiry. To our knowledge, this antibody has yet to be tested in this application. All tested applications are specified on Abcam product datasheets. If you decide to go ahead and purchase this product, please let us know how you get...

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    Thank you for your patience. I was away on vacation and thus could not get back to you earlier. 1) Using ab6276, you are most likely detecting a dimer. I would suggest trying the antibody with different samples to ensure that the antibody detects th...

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    The originator of ab6665 informed me that the band close to 70kDa should be the specific band. The others are non-specific. This result is very consistent with what they have previously seen. They don't have a recommendation for a positive control but ...

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    As you probably know Cox-2 (Cyclooxygenase-2) is an inducible enzyme that is normally absent from cells, however, in response to growth factors, tumour promoters and some cytokines, it undergoes a rapid and transient expression. We are very sorry t...

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    I don't recall that any of our products have been tested for cross reactivity with bat! ab6665 has not yet been tested in IHC. I suggest that you take a look at the IHC and antigen retrieval protocols on our the Abcam website. This is the only antibody...

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    ab6665 is a polyclonal so it should be fine in your application (polyclonals recognize multiple epitopes)

    This antibody has not been tested in immunohistochemistry, or with horse tissue/cells. We would like to offer you a test sample, however unfortunately we do not have any available for this antibody. It's difficult to predict the performance of an antib...

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    The sensitivity of this antiserum is not determined.


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