Recombinant Anti-COX2 / Cyclooxygenase 2 antibody [EP1978Y] (ab62331)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1978Y] to COX2 / Cyclooxygenase 2
- Suitable for: WB
- Reacts with: Mouse, Human
Overview
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Product name
Anti-COX2 / Cyclooxygenase 2 antibody [EP1978Y]
See all COX2 / Cyclooxygenase 2 primary antibodies -
Description
Rabbit monoclonal [EP1978Y] to COX2 / Cyclooxygenase 2 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt or ICC/IF -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
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Positive control
- WB: Raw264.7 cell lysate + LPS.
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General notes
Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Abcam recommends ab179800 for use in ICC/IF.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Dissociation constant (KD)
KD = 1.01 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP1978Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Immunizing Peptide (Blocking)
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab62331 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/1000. Detects a band of approximately 72 kDa (predicted molecular weight: 69 kDa).Can be blocked with COX2 / Cyclooxygenase 2 peptide (ab213704). |
Target
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Function
Mediates the formation of prostaglandins from arachidonate. May have a role as a major mediator of inflammation and/or a role for prostanoid signaling in activity-dependent plasticity. -
Pathway
Lipid metabolism; prostaglandin biosynthesis. -
Sequence similarities
Belongs to the prostaglandin G/H synthase family.
Contains 1 EGF-like domain. -
Post-translational
modificationsS-nitrosylation by NOS2 (iNOS) activates enzme activity. S-nitrosylation may take place on different Cys residues in addition to Cys-561. -
Cellular localization
Microsome membrane. Endoplasmic reticulum membrane. - Information by UniProt
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Database links
- Entrez Gene: 5743 Human
- Entrez Gene: 19225 Mouse
- Omim: 600262 Human
- SwissProt: P35354 Human
- SwissProt: Q05769 Mouse
- Unigene: 196384 Human
- Unigene: 292547 Mouse
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Alternative names
- COX 2 antibody
- COX-2 antibody
- COX2 antibody
see all
Images
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![Western blot - Anti-COX2 / Cyclooxygenase 2 antibody [EP1978Y] (ab62331)](https://www.abcam.com/ps/products/62/ab62331/Images/ab62331-284754-anti-cox2-cyclooxygenase-2-antibody-ep1978y-western-blot.jpg)
Western blot - Anti-COX2 / Cyclooxygenase 2 antibody [EP1978Y] (ab62331)All lanes : Anti-COX2 / Cyclooxygenase 2 antibody [EP1978Y] (ab62331) at 1/1000 dilution (purified)
Lane 1 : Untreated Raw 264.7 cell lysate
Lane 2 : Raw 264.7 treated with LPS
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG (H+L) at 1/50000 dilution
Predicted band size: 69 kDa
Observed band size: 72 kDa why is the actual band size different from the predicted?Blocking and dilution buffer: 5% NFDM/TBST
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![Western blot - Anti-COX2 / Cyclooxygenase 2 antibody [EP1978Y] (ab62331)](https://www.abcam.com/ps/products/62/ab62331/Images/ab62331-332354-anti-cox2-cyclooxygenase-2-antibody-ep1978y-western-blot.jpg)
Western blot - Anti-COX2 / Cyclooxygenase 2 antibody [EP1978Y] (ab62331)All lanes : Anti-COX2 / Cyclooxygenase 2 antibody [EP1978Y] (ab62331) at 1/1000 dilution (Purified)
Lane 1 : HCT 116 (human colorectal carcinoma cell line) whole cell lysate
Lane 2 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
Lane 3 : U-87 MG (human glioblastoma-astrocytoma epithelial cell line) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
Predicted band size: 69 kDa
Observed band size: 72 kDa why is the actual band size different from the predicted?Blocking and dilution buffer: 5% NFDM/TBST.
Exposure times
Lane 1 and 2: 5.5 seconds
Lane 3: 3.25 secondsWe are unsure as to the
identity of the extra band in HCT 116 and MCF7.
The expression profile observed in HCT 116 and MCF7 are consistent with the literatures (PMID: 14739610, PMID: 24325753, PMID: 16997132).
Negative control: HCT 116 (PMID: 14739610) and MCF7 (PMID: 24325753, PMID: 16997132) -
![Western blot - Anti-COX2 / Cyclooxygenase 2 antibody [EP1978Y] (ab62331)](https://www.abcam.com/ps/products/62/ab62331/Images/ab62331-75633-anti-cox2-cyclooxygenase-2-antibody-ep1978y-western-blot.jpg)
Western blot - Anti-COX2 / Cyclooxygenase 2 antibody [EP1978Y] (ab62331)Anti-COX2 / Cyclooxygenase 2 antibody [EP1978Y] (ab62331) at 1/500 dilution (unpurified) + Raw264.7 cell lysate (10ug) + LPS
Secondary
Goat anti-Rabbit HRP conjugated at 1/2000 dilution
Predicted band size: 69 kDa
Observed band size: 72 kDa why is the actual band size different from the predicted?
Protocols
References (37)
ab62331 has been referenced in 37 publications.
- Qin T et al. Prediction of the mechanisms of action of Shenkang in chronic kidney disease: A network pharmacology study and experimental validation. J Ethnopharmacol 246:112128 (2020). PubMed: 31386888
- Han C et al. PHLDA1 promotes microglia-mediated neuroinflammation via regulating K63-linked ubiquitination of TRAF6. Brain Behav Immun N/A:N/A (2020). PubMed: 32353516
- Metryka E et al. Lead (Pb) as a Factor Initiating and Potentiating Inflammation in Human THP-1 Macrophages. Int J Mol Sci 21:N/A (2020). PubMed: 32214022
- Xie R et al. c-Myb and its Effector COX-2 as an Indicator Associated with Prognosis and Therapeutic Outcome in Colorectal Cancer. J Cancer 10:1601-1610 (2019). PubMed: 31205515
- Li Y et al. Ischemia-induced ACSL4 activation contributes to ferroptosis-mediated tissue injury in intestinal ischemia/reperfusion. Cell Death Differ N/A:N/A (2019). PubMed: 30737476
