Recombinant
RabMAb

Recombinant Anti-COX2 / Cyclooxygenase 2 antibody [EP1978Y] (ab62331)

Overview

  • Product name

    Anti-COX2 / Cyclooxygenase 2 antibody [EP1978Y]
    See all COX2 / Cyclooxygenase 2 primary antibodies
  • Description

    Rabbit monoclonal [EP1978Y] to COX2 / Cyclooxygenase 2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt or ICC/IF
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human COX2/ Cyclooxygenase 2 aa 100-200. The exact sequence is proprietary.
    Database link: P35354
    (Peptide available as ab213704)

  • Positive control

    • WB: Raw264.7 cell lysate + LPS.
  • General notes

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Abcam recommends ab179800 for use in ICC/IF.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Applications

Our Abpromise guarantee covers the use of ab62331 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 72 kDa (predicted molecular weight: 69 kDa).Can be blocked with COX2 / Cyclooxygenase 2 peptide (ab213704).
  • Application notes
    Is unsuitable for Flow Cyt or ICC/IF.
  • Target

    • Function

      Mediates the formation of prostaglandins from arachidonate. May have a role as a major mediator of inflammation and/or a role for prostanoid signaling in activity-dependent plasticity.
    • Pathway

      Lipid metabolism; prostaglandin biosynthesis.
    • Sequence similarities

      Belongs to the prostaglandin G/H synthase family.
      Contains 1 EGF-like domain.
    • Post-translational
      modifications

      S-nitrosylation by NOS2 (iNOS) activates enzme activity. S-nitrosylation may take place on different Cys residues in addition to Cys-561.
    • Cellular localization

      Microsome membrane. Endoplasmic reticulum membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • COX 2 antibody
      • COX-2 antibody
      • COX2 antibody
      • Cyclooxygenase 2 antibody
      • Cyclooxygenase 2b antibody
      • Cyclooxygenase antibody
      • Cyclooxygenase-2 antibody
      • Cyclooxygenase2 antibody
      • EC 1.14.99.1 antibody
      • fj02a10 antibody
      • Glucocorticoid-regulated inflammatory cyclooxygenase antibody
      • Glucocorticoid-regulated inflammatory Prostaglandin G/H synthase antibody
      • GRIPGHS antibody
      • hCox 2 antibody
      • Macrophage activation-associated marker protein P71/73 antibody
      • OTTHUMP00000033524 antibody
      • PES-2 antibody
      • PGG/HS antibody
      • PGH synthase 2 antibody
      • PGH2_HUMAN antibody
      • PGHS 2 antibody
      • PGHS-2 antibody
      • PGHS2 antibody
      • PHS 2 antibody
      • PHS II antibody
      • PHS2 antibody
      • Prostaglandin endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase) antibody
      • Prostaglandin endoperoxide synthase 2 antibody
      • Prostaglandin G/H synthase 2 antibody
      • Prostaglandin G/H synthase 2 precursor antibody
      • Prostaglandin G/H synthase and cyclooxygenase antibody
      • Prostaglandin G/H synthase antibody
      • Prostaglandin H2 synthase 2 antibody
      • prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase) antibody
      • Prostaglandin-endoperoxide synthase 2 antibody
      • PTGS2 antibody
      • ptgs2a antibody
      • TIS10 antibody
      • TIS10 protein antibody
      • unp1239 antibody
      • wu:fj02a10 antibody
      see all

    Images

    • All lanes : Anti-COX2 / Cyclooxygenase 2 antibody [EP1978Y] (ab62331) at 1/1000 dilution (purified)

      Lane 1 : Untreated Raw 264.7 cell lysate
      Lane 2 : Raw 264.7 treated with LPS

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP-conjugated goat anti-rabbit IgG (H+L) at 1/50000 dilution

      Predicted band size: 69 kDa
      Observed band size: 72 kDa
      why is the actual band size different from the predicted?



      Blocking and dilution buffer: 5% NFDM/TBST

    • All lanes : Anti-COX2 / Cyclooxygenase 2 antibody [EP1978Y] (ab62331) at 1/1000 dilution (Purified)

      Lane 1 : HCT 116 (human colorectal carcinoma cell line) whole cell lysate with 5% NFDM/TBST
      Lane 2 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate with 5% NFDM/TBST
      Lane 3 : U-87 MG (human glioblastoma-astrocytoma epithelial cell line) whole cell lysate with 5% NFDM/TBST

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051)

      Predicted band size: 69 kDa
      Observed band size: 72 kDa why is the actual band size different from the predicted?



      Exposure times

      Lane 1 and 2: 5.5 seconds
      Lane 3: 3.25 seconds

      We are unsure as to the
      identity of the extra band in HCT 116 and MCF7.
      The expression profile observed in HCT 116 and MCF7 are consistent with the literatures (PMID: 14739610, PMID: 24325753, PMID: 16997132).
      Negative control: HCT 116 (PMID: 14739610) and MCF7 (PMID: 24325753, PMID: 16997132)

    • Anti-COX2 / Cyclooxygenase 2 antibody [EP1978Y] (ab62331) at 1/500 dilution (unpurified) + Raw264.7 cell lysate (10ug) + LPS

      Secondary
      Goat anti-Rabbit HRP conjugated

      Predicted band size: 69 kDa
      Observed band size: 72 kDa why is the actual band size different from the predicted?

    References

    This product has been referenced in:

    • Qin T  et al. Prediction of the mechanisms of action of Shenkang in chronic kidney disease: A network pharmacology study and experimental validation. J Ethnopharmacol 246:112128 (2020). Read more (PubMed: 31386888) »
    • Xie R  et al. c-Myb and its Effector COX-2 as an Indicator Associated with Prognosis and Therapeutic Outcome in Colorectal Cancer. J Cancer 10:1601-1610 (2019). Read more (PubMed: 31205515) »
    See all 29 Publications for this product

    Customer reviews and Q&As

    1-10 of 11 Abreviews or Q&A

    Application
    Western blot
    Sample
    Dog Cell lysate - whole cell (melanoma cell line)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    10 µg
    Specification
    melanoma cell line
    Blocking step
    (agent) for 50 minute(s) · Concentration: 100% · Temperature: 25°C

    Dr. 令 中野

    Verified customer

    Submitted Jul 02 2019

    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Mouse Tissue sections (Brain)
    Antigen retrieval step
    None
    Permeabilization
    No
    Specification
    Brain
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: RT°C
    Fixative
    Paraformaldehyde

    Dr. Robert Fenster

    Verified customer

    Submitted Aug 24 2018

    Application
    Western blot
    Sample
    Baboon Tissue lysate - whole (Placenta samples and liver samples)
    Gel Running Conditions
    Reduced Denaturing (12% Gel)
    Loading amount
    40 µg
    Specification
    Placenta samples and liver samples
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C

    Dr. Kushal Gandhi

    Verified customer

    Submitted Sep 06 2017

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    Serum as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Tris EDTA pH 9
    Sample
    Dog Tissue sections (MDCK pellets in paraffin)
    Specification
    MDCK pellets in paraffin
    Permeabilization
    Yes - Tween-20
    Fixative
    Paraformaldehyde

    Abcam user community

    Verified customer

    Submitted Jan 05 2015

    This product is known to not work in this application or species.
    Application
    Immunocytochemistry/ Immunofluorescence
    Blocking step
    BSA as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
    Sample
    Mouse Cell (L cells)
    Specification
    L cells
    Permeabilization
    Yes - Triton 1%
    Fixative
    Paraformaldehyde

    Abcam user community

    Verified customer

    Submitted Dec 10 2014

    This product is known to not work in this application or species.
    Application
    Immunocytochemistry/ Immunofluorescence
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 1°C
    Sample
    Human Cell (293T)
    Specification
    293T
    Permeabilization
    Yes - Triton 1%
    Fixative
    Paraformaldehyde

    Abcam user community

    Verified customer

    Submitted Nov 20 2014

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    FBS / BSA as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 1%
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Tris pH 9
    Sample
    Human Tissue sections (Infantile fibromatosis)
    Specification
    Infantile fibromatosis
    Permeabilization
    No
    Fixative
    Formaldehyde

    Abcam user community

    Verified customer

    Submitted Jul 04 2014

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    1%BSA / 1% FBS in TBS as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Tris buffer pH 9
    Sample
    Mouse Tissue sections (Mouse 14.5 dpc)
    Specification
    Mouse 14.5 dpc
    Permeabilization
    Yes - TBS-T
    Fixative
    Formaldehyde

    Abcam user community

    Verified customer

    Submitted Sep 25 2013

    This product is known to not work in this application or species.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa)
    Specification
    HeLa
    Permeabilization
    Yes - 0.5% Triton X100 in PBS
    Fixative
    Paraformaldehyde

    Dr. Kirk Mcmanus

    Verified customer

    Submitted Aug 29 2013

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (pancreas)
    Specification
    pancreas
    Fixative
    Formaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Sodium Citrate
    Permeabilization
    No
    Blocking step
    GOAT BLOCK = 10mM Tris pH 7.4, 100mM MgCl2, 0.5% Tween, 1% BSA, 5% normal goat serum (IN WATER) as blocking agent for 20 minute(s) · Concentration: 100% · Temperature: 25°C

    Abcam user community

    Verified customer

    Submitted Apr 11 2013

    1-10 of 11 Abreviews or Q&A

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