Anti-COX2 / Cyclooxygenase 2 antibody [EP8588] (ab169782)
![Western blot - Anti-COX2 / Cyclooxygenase 2 antibody [EP8588] (ab169782)](https://www.abcam.com/ps/products/169/ab169782/Images/ab169782-4-anti-cox2-cyclooxygenase-2-antibody-ep8588-western-blot-human-lysate.jpg "Western blot - Anti-COX2 / Cyclooxygenase 2 antibody [EP8588] (ab169782)")
Key features and details
- Rabbit monoclonal [EP8588] to COX2 / Cyclooxygenase 2
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-COX2 / Cyclooxygenase 2 antibody [EP8588]
See all COX2 / Cyclooxygenase 2 primary antibodies -
Description
Rabbit monoclonal [EP8588] to COX2 / Cyclooxygenase 2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt,ICC/IF or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Sheep, Rabbit, Horse, Cow, Mink
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Immunogen
Recombinant fragment within Human COX2/ Cyclooxygenase 2 aa 500-600. The exact sequence is proprietary.
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Positive control
- WB: Recombinant Human COX2 / Cyclooxygenase 2 protein (ab159279); A549, U-87 MG and HeLa cell lysates; Lysate from THP1 cells treated with LPS; Human breast carcinoma and tonsil tissues.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EP8588 -
Isotype
IgG -
Research areas
Associated products
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Isotype control
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KO cell lines
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KO cell lysates
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KO cell pellets
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab169782 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/5000. Predicted molecular weight: 69 kDa.
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| IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| Notes |
|---|
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WB
1/1000 - 1/5000. Predicted molecular weight: 69 kDa. |
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IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Mediates the formation of prostaglandins from arachidonate. May have a role as a major mediator of inflammation and/or a role for prostanoid signaling in activity-dependent plasticity. -
Pathway
Lipid metabolism; prostaglandin biosynthesis. -
Sequence similarities
Belongs to the prostaglandin G/H synthase family.
Contains 1 EGF-like domain. -
Post-translational
modificationsS-nitrosylation by NOS2 (iNOS) activates enzme activity. S-nitrosylation may take place on different Cys residues in addition to Cys-561. -
Cellular localization
Microsome membrane. Endoplasmic reticulum membrane. - Information by UniProt
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Database links
- Entrez Gene: 282023 Cow
- Entrez Gene: 5743 Human
- Entrez Gene: 443460 Sheep
- Omim: 600262 Human
- SwissProt: O62698 Cow
- SwissProt: O19183 Horse
- SwissProt: P35354 Human
- SwissProt: O02768 Rabbit
see all -
Alternative names
- COX 2 antibody
- COX-2 antibody
- COX2 antibody
see all
Images
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Western blot - Anti-COX2 / Cyclooxygenase 2 antibody [EP8588] (ab169782)All lanes : Anti-COX2 / Cyclooxygenase 2 antibody [EP8588] (ab169782) at 1/1000 dilution
Lane 1 : A549 cell lysate
Lane 2 : U-87 MG cell lysate
Lane 3 : Wild-type HeLa cell lysate
Lane 4 : PTGS2 knockout HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 69 kDaLanes 1 - 4: Merged signal (red and green). Green - ab169782 observed at 75 kDa. Red - loading control, ab8245 observed at 37 kDa.
ab169782 was shown to react with COX2 / Cyclooxygenase 2 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab255420 (knockout cell lysate ab263795) was used. Wild-type and COX2 / Cyclooxygenase 2 knockout samples were subjected to SDS-PAGE. ab169782 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX2 / Cyclooxygenase 2 antibody [EP8588] (ab169782)](https://www.abcam.com/ps/products/169/ab169782/Images/ab169782-168450-anti-cox2-cyclooxygenase-2-antibody-ep8588-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX2 / Cyclooxygenase 2 antibody [EP8588] (ab169782)Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human breast carcinoma tissue, labeling COX2/ Cyclooxygenase 2 using ab169782 at a 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Western blot - Anti-COX2 / Cyclooxygenase 2 antibody [EP8588] (ab169782)](https://www.abcam.com/ps/products/169/ab169782/Images/ab169782-168451-anti-cox2-cyclooxygenase-2-antibody-ep8588-western-blot.jpg)
Western blot - Anti-COX2 / Cyclooxygenase 2 antibody [EP8588] (ab169782)All lanes : Anti-COX2 / Cyclooxygenase 2 antibody [EP8588] (ab169782) at 1/1000 dilution
Lane 1 : A549 cell lysate
Lane 2 : Lysate from THP1 cells treated with LPS
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 69 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX2 / Cyclooxygenase 2 antibody [EP8588] (ab169782)](https://www.abcam.com/ps/products/169/ab169782/Images/ab169782-168449-anti-cox2-cyclooxygenase-2-antibody-ep8588-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX2 / Cyclooxygenase 2 antibody [EP8588] (ab169782)Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human tonsil tissue, labeling COX2/ Cyclooxygenase 2 using ab169782 at a 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab169782 has been referenced in 2 publications.
- Zhang H et al. Long-term use of fluoxetine accelerates bone loss through the disruption of sphingolipids metabolism in bone marrow adipose tissue. Transl Psychiatry 10:138 (2020). PubMed: 32398744
- Jin J et al. Pirfenidone attenuates lung fibrotic fibroblast responses to transforming growth factor-ß1. Respir Res 20:119 (2019). PubMed: 31185973