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  1. Link

    cox2--cyclooxygenase-2-antibody-ep8588-ab169782.pdf

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Immunology Innate Immunity Macrophage / Inflamm.
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Validated using a knockout cell lineRabMAb

Anti-COX2 / Cyclooxygenase 2 antibody [EP8588] (ab169782)

  • Datasheet
Reviews (1) Submit a question References (4)

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Western blot - Anti-COX2 / Cyclooxygenase 2 antibody [EP8588] (ab169782)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX2 / Cyclooxygenase 2 antibody [EP8588] (ab169782)
  • Western blot - Anti-COX2 / Cyclooxygenase 2 antibody [EP8588] (ab169782)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX2 / Cyclooxygenase 2 antibody [EP8588] (ab169782)

Key features and details

  • Rabbit monoclonal [EP8588] to COX2 / Cyclooxygenase 2
  • Suitable for: WB, IHC-P
  • Knockout validated
  • Reacts with: Human
  • Isotype: IgG

Conjugates logo Related conjugates and formulations

Carrier Free

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Primary
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Anti-Resistin antibody (ab119501)
Protein
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Recombinant human COX2 / Cyclooxygenase 2 protein (ab198646)
ELISA
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Mouse Resistin ELISA Kit (ab205574)

View more associated products

Overview

  • Product name

    Anti-COX2 / Cyclooxygenase 2 antibody [EP8588]
    See all COX2 / Cyclooxygenase 2 primary antibodies
  • Description

    Rabbit monoclonal [EP8588] to COX2 / Cyclooxygenase 2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC/IF or IP
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Sheep, Rabbit, Horse, Cow, Mink
  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Recombinant Human COX2 / Cyclooxygenase 2 protein (ab159279); A549, U-87 MG and HeLa cell lysates; Lysate from THP1 cells treated with LPS IHC-P: Human breast carcinoma and tonsil tissues.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EP8588
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Innate Immunity
    • Macrophage / Inflamm.
    • Cardiovascular
    • Blood
    • Platelets
    • Cardiovascular
    • Lipids / Lipoproteins
    • Lipid Metabolism
    • Cholesterol Metabolism
    • Signal Transduction
    • Signaling Pathway
    • Lipid Signaling
    • Prostaglandins
    • Cancer
    • Tumor biomarkers
    • Other
    • Cardiovascular
    • Atherosclerosis
    • Lipoprotein metabolism
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Hormone biosynthesis
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Cholesterol Metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Lipoprotein metabolism
    • Metabolism
    • Pathways and Processes
    • Endocrine metabolism
    • Hormone biosynthesis
    • Metabolism
    • Types of disease
    • Heart disease
    • Neuroscience
    • Processes

Associated products

  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • KO cell lines

    • Human PTGS2 (COX2 / Cyclooxygenase 2) knockout HeLa cell line (ab255420)
  • KO cell lysates

    • Human PTGS2 (COX2 / Cyclooxygenase 2) knockout HeLa cell lysate (ab263795)
  • KO cell pellets

    • Human PTGS2 (COX2 / Cyclooxygenase 2) knockout HeLa cell pellet (ab278922)
  • Positive Controls

    • Recombinant Human COX2 / Cyclooxygenase 2 protein (ab159279)
    • A549 whole cell lysate (ab7910)
  • Recombinant Protein

    • Recombinant human COX2 / Cyclooxygenase 2 protein (ab198646)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab169782 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/5000. Predicted molecular weight: 69 kDa.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Notes
WB
1/1000 - 1/5000. Predicted molecular weight: 69 kDa.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Application notes
Is unsuitable for Flow Cyt,ICC/IF or IP.

Target

  • Function

    Mediates the formation of prostaglandins from arachidonate. May have a role as a major mediator of inflammation and/or a role for prostanoid signaling in activity-dependent plasticity.
  • Pathway

    Lipid metabolism; prostaglandin biosynthesis.
  • Sequence similarities

    Belongs to the prostaglandin G/H synthase family.
    Contains 1 EGF-like domain.
  • Post-translational
    modifications

    S-nitrosylation by NOS2 (iNOS) activates enzme activity. S-nitrosylation may take place on different Cys residues in addition to Cys-561.
  • Cellular localization

    Microsome membrane. Endoplasmic reticulum membrane.
  • Target information above from: UniProt accession P35354 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 282023 Cow
    • Entrez Gene: 5743 Human
    • Entrez Gene: 443460 Sheep
    • Omim: 600262 Human
    • SwissProt: O62698 Cow
    • SwissProt: O19183 Horse
    • SwissProt: P35354 Human
    • SwissProt: O02768 Rabbit
    • SwissProt: P79208 Sheep
    • Unigene: 196384 Human
    see all
  • Alternative names

    • COX 2 antibody
    • COX-2 antibody
    • COX2 antibody
    • Cyclooxygenase 2 antibody
    • Cyclooxygenase 2b antibody
    • Cyclooxygenase antibody
    • Cyclooxygenase-2 antibody
    • Cyclooxygenase2 antibody
    • EC 1.14.99.1 antibody
    • fj02a10 antibody
    • Glucocorticoid-regulated inflammatory cyclooxygenase antibody
    • Glucocorticoid-regulated inflammatory Prostaglandin G/H synthase antibody
    • GRIPGHS antibody
    • hCox 2 antibody
    • Macrophage activation-associated marker protein P71/73 antibody
    • OTTHUMP00000033524 antibody
    • PES-2 antibody
    • PGG/HS antibody
    • PGH synthase 2 antibody
    • PGH2_HUMAN antibody
    • PGHS 2 antibody
    • PGHS-2 antibody
    • PGHS2 antibody
    • PHS 2 antibody
    • PHS II antibody
    • PHS2 antibody
    • Prostaglandin endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase) antibody
    • Prostaglandin endoperoxide synthase 2 antibody
    • Prostaglandin G/H synthase 2 antibody
    • Prostaglandin G/H synthase 2 precursor antibody
    • Prostaglandin G/H synthase and cyclooxygenase antibody
    • Prostaglandin G/H synthase antibody
    • Prostaglandin H2 synthase 2 antibody
    • prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase) antibody
    • Prostaglandin-endoperoxide synthase 2 antibody
    • PTGS2 antibody
    • ptgs2a antibody
    • TIS10 antibody
    • TIS10 protein antibody
    • unp1239 antibody
    • wu:fj02a10 antibody
    see all

Images

  • Western blot - Anti-COX2 / Cyclooxygenase 2 antibody [EP8588] (ab169782)
    Western blot - Anti-COX2 / Cyclooxygenase 2 antibody [EP8588] (ab169782)
    All lanes : Anti-COX2 / Cyclooxygenase 2 antibody [EP8588] (ab169782) at 1/1000 dilution

    Lane 1 : A549 cell lysate
    Lane 2 : U-87 MG cell lysate
    Lane 3 : Wild-type HeLa cell lysate
    Lane 4 : PTGS2 knockout HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 69 kDa



    Lanes 1 - 4: Merged signal (red and green). Green - ab169782 observed at 75 kDa. Red - loading control, ab8245 observed at 37 kDa.

    ab169782 was shown to react with COX2 / Cyclooxygenase 2 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab255420 (knockout cell lysate ab263795) was used. Wild-type and COX2 / Cyclooxygenase 2 knockout samples were subjected to SDS-PAGE. ab169782 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX2 / Cyclooxygenase 2 antibody [EP8588] (ab169782)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX2 / Cyclooxygenase 2 antibody [EP8588] (ab169782)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human breast carcinoma tissue, labeling COX2/ Cyclooxygenase 2 using ab169782 at a 1/50 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Western blot - Anti-COX2 / Cyclooxygenase 2 antibody [EP8588] (ab169782)
    Western blot - Anti-COX2 / Cyclooxygenase 2 antibody [EP8588] (ab169782)
    All lanes : Anti-COX2 / Cyclooxygenase 2 antibody [EP8588] (ab169782) at 1/1000 dilution

    Lane 1 : A549 cell lysate
    Lane 2 : Lysate from THP1 cells treated with LPS

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 69 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX2 / Cyclooxygenase 2 antibody [EP8588] (ab169782)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX2 / Cyclooxygenase 2 antibody [EP8588] (ab169782)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human tonsil tissue, labeling COX2/ Cyclooxygenase 2 using ab169782 at a 1/50 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Protocols

  • Western blot protocols
  • Immunohistochemistry protocols

Click here to view the general protocols

Datasheets and documents

  • Datasheet download

    Download

References (4)

Publishing research using ab169782? Please let us know so that we can cite the reference in this datasheet.

ab169782 has been referenced in 4 publications.

  • Zhu L  et al. Identification the ferroptosis-related gene signature in patients with esophageal adenocarcinoma. Cancer Cell Int 21:124 (2021). PubMed: 33602233
  • Wang J  et al. Involvement of the VEGF signaling pathway in immunosuppression and hypoxia stress: analysis of mRNA expression in lymphocytes mediating panting in Jersey cattle under heat stress. BMC Vet Res 17:209 (2021). PubMed: 34098948
  • Zhang H  et al. Long-term use of fluoxetine accelerates bone loss through the disruption of sphingolipids metabolism in bone marrow adipose tissue. Transl Psychiatry 10:138 (2020). PubMed: 32398744
  • Jin J  et al. Pirfenidone attenuates lung fibrotic fibroblast responses to transforming growth factor-ß1. Respir Res 20:119 (2019). PubMed: 31185973

Customer reviews and Q&As

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-COX2 / Cyclooxygenase 2 antibody [EP8588]

Inconclusive
Abreviews
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Sheep Tissue sections (Bone & Kidney)
Antigen retrieval step
Enzymatic - Buffer/Enzyme Used: Tested Trypsin and Pepsinn
Permeabilization
Yes - 0.025% Triton X100
Specification
Bone & Kidney
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 20% · Temperature: 23°C
Fixative
Formaldehyde
Read More

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Submitted Mar 19 2020

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