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    cox2--cyclooxygenase-2-antibody-epr12012-ab179800.pdf

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Immunology Innate Immunity Macrophage / Inflamm.
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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-COX2 / Cyclooxygenase 2 antibody [EPR12012] (ab179800)

  • Datasheet
  • SDS
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Western blot - Anti-COX2 / Cyclooxygenase 2 antibody [EPR12012] (ab179800)
  • Western blot - Anti-COX2 / Cyclooxygenase 2 antibody [EPR12012] (ab179800)
  • Western blot - Anti-COX2 / Cyclooxygenase 2 antibody [EPR12012] (ab179800)
  • Western blot - Anti-COX2 / Cyclooxygenase 2 antibody [EPR12012] (ab179800)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX2 / Cyclooxygenase 2 antibody [EPR12012] (ab179800)
  • Immunocytochemistry/ Immunofluorescence - Anti-COX2 / Cyclooxygenase 2 antibody [EPR12012] (ab179800)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX2 / Cyclooxygenase 2 antibody [EPR12012] (ab179800)
  • Western blot - Anti-COX2 / Cyclooxygenase 2 antibody [EPR12012] (ab179800)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX2 / Cyclooxygenase 2 antibody [EPR12012] (ab179800)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX2 / Cyclooxygenase 2 antibody [EPR12012] (ab179800)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX2 / Cyclooxygenase 2 antibody [EPR12012] (ab179800)
  • Western blot - Anti-COX2 / Cyclooxygenase 2 antibody [EPR12012] (ab179800)
  • Western blot - Anti-COX2 / Cyclooxygenase 2 antibody [EPR12012] (ab179800)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX2 / Cyclooxygenase 2 antibody [EPR12012] (ab179800)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX2 / Cyclooxygenase 2 antibody [EPR12012] (ab179800)
  • Immunoprecipitation - Anti-COX2 / Cyclooxygenase 2 antibody [EPR12012] (ab179800)
  • Western blot - Anti-COX2 / Cyclooxygenase 2 antibody [EPR12012] (ab179800)
  • Immunoprecipitation - Anti-COX2 / Cyclooxygenase 2 antibody [EPR12012] (ab179800)
  • Anti-COX2 / Cyclooxygenase 2 antibody [EPR12012] (ab179800)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12012] to COX2 / Cyclooxygenase 2
  • Suitable for: WB, IP, ICC/IF, IHC-P
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 647 Carrier Free

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Anti-HPRT antibody [EPR5298] (ab133242)
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Alexa Fluor® 647 Anti-COX2 / Cyclooxygenase 2 antibody [EPR12012] (ab225273)
Knockout
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Human PTGS2 (COX2 / Cyclooxygenase 2) knockout HeLa cell line (ab255420)

View more associated products

Overview

  • Product name

    Anti-COX2 / Cyclooxygenase 2 antibody [EPR12012]
    See all COX2 / Cyclooxygenase 2 primary antibodies
  • Description

    Rabbit monoclonal [EPR12012] to COX2 / Cyclooxygenase 2
  • Host species

    Rabbit
  • Specificity

    Stimulation is required to allow detection of the COX2 protein in some cell lines and tissues. It is better to use a positive control side by side when testing.

    Rat species is recommended based on IHC result, we do not guarantee WB, IP and ICC/IF for Rat.
  • Tested applications

    Suitable for: WB, IP, ICC/IF, IHC-Pmore details
    Unsuitable for: Flow Cyt
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: A549, U-87 MG and HeLa cell lysates; mouse spleen tissue lysate, PTGS2 (COX2 / Cyclooxygenase 2) KO A549 (Human lung carcinoma epithelial cell) cell lysate, Wild-type A549 cell lysate, U-87 MG whole cell lysate, MCF7 whole cell lysate. Mouse B16-F10 and Raw 264.7 whole cell lysate. Mouse retina, hippocampus, heart and kidney tissue lysate. IHC-P: Human colonic carcinoma, lung carcinoma, liver and colon tissues: rat kidney tissue; mouse kidney and liver tissue. IP: A549 cell lysate ICC: U-87 MG cells
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR12012
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Innate Immunity
    • Macrophage / Inflamm.
    • Cardiovascular
    • Blood
    • Platelets
    • Cardiovascular
    • Lipids / Lipoproteins
    • Lipid Metabolism
    • Cholesterol Metabolism
    • Signal Transduction
    • Signaling Pathway
    • Lipid Signaling
    • Prostaglandins
    • Cancer
    • Tumor biomarkers
    • Other
    • Cardiovascular
    • Atherosclerosis
    • Lipoprotein metabolism
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Hormone biosynthesis
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Cholesterol Metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Lipoprotein metabolism
    • Metabolism
    • Pathways and Processes
    • Endocrine metabolism
    • Hormone biosynthesis
    • Metabolism
    • Types of disease
    • Heart disease
    • Neuroscience
    • Processes

Associated products

  • Alternative Versions

    • Alexa Fluor® 647 Anti-COX2 / Cyclooxygenase 2 antibody [EPR12012] (ab225273)
    • Anti-COX2 / Cyclooxygenase 2 antibody [EPR12012] - BSA and Azide free (ab227528)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773)
    • Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • KO cell lines

    • Human PTGS2 (COX2 / Cyclooxygenase 2) knockout HeLa cell line (ab255420)
  • KO cell lysates

    • Human PTGS2 (COX2 / Cyclooxygenase 2) knockout HeLa cell lysate (ab263795)
  • KO cell pellets

    • Human PTGS2 (COX2 / Cyclooxygenase 2) knockout HeLa cell pellet (ab278922)
  • Positive Controls

    • Mouse spleen tissue lysate - total protein (ab29293)
    • Mouse spleen tissue lysate - total protein (14 days) (ab7274)
    • A549 whole cell lysate (ab7910)
  • Recombinant Protein

    • Recombinant human COX2 / Cyclooxygenase 2 protein (ab198646)
  • Related Products

    • Recombinant Human COX2 / Cyclooxygenase 2 protein (ab159279)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab179800 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (1)
1/1000 - 1/5000. Predicted molecular weight: 69 kDa.
IP
1/10 - 1/100.
ICC/IF
Use at an assay dependent concentration.
IHC-P (2)
1/100 - 1/4000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

Notes
WB
1/1000 - 1/5000. Predicted molecular weight: 69 kDa.
IP
1/10 - 1/100.
ICC/IF
Use at an assay dependent concentration.
IHC-P
1/100 - 1/4000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

Application notes
Is unsuitable for Flow Cyt.

Target

  • Function

    Mediates the formation of prostaglandins from arachidonate. May have a role as a major mediator of inflammation and/or a role for prostanoid signaling in activity-dependent plasticity.
  • Pathway

    Lipid metabolism; prostaglandin biosynthesis.
  • Sequence similarities

    Belongs to the prostaglandin G/H synthase family.
    Contains 1 EGF-like domain.
  • Post-translational
    modifications

    S-nitrosylation by NOS2 (iNOS) activates enzme activity. S-nitrosylation may take place on different Cys residues in addition to Cys-561.
  • Cellular localization

    Microsome membrane. Endoplasmic reticulum membrane.
  • Target information above from: UniProt accession P35354 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 5743 Human
    • Entrez Gene: 19225 Mouse
    • Entrez Gene: 29527 Rat
    • Omim: 600262 Human
    • SwissProt: P35354 Human
    • SwissProt: Q05769 Mouse
    • SwissProt: P35355 Rat
    • Unigene: 196384 Human
    • Unigene: 292547 Mouse
    • Unigene: 44369 Rat
    see all
  • Alternative names

    • COX 2 antibody
    • COX-2 antibody
    • COX2 antibody
    • Cyclooxygenase 2 antibody
    • Cyclooxygenase 2b antibody
    • Cyclooxygenase antibody
    • Cyclooxygenase-2 antibody
    • Cyclooxygenase2 antibody
    • EC 1.14.99.1 antibody
    • fj02a10 antibody
    • Glucocorticoid-regulated inflammatory cyclooxygenase antibody
    • Glucocorticoid-regulated inflammatory Prostaglandin G/H synthase antibody
    • GRIPGHS antibody
    • hCox 2 antibody
    • Macrophage activation-associated marker protein P71/73 antibody
    • OTTHUMP00000033524 antibody
    • PES-2 antibody
    • PGG/HS antibody
    • PGH synthase 2 antibody
    • PGH2_HUMAN antibody
    • PGHS 2 antibody
    • PGHS-2 antibody
    • PGHS2 antibody
    • PHS 2 antibody
    • PHS II antibody
    • PHS2 antibody
    • Prostaglandin endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase) antibody
    • Prostaglandin endoperoxide synthase 2 antibody
    • Prostaglandin G/H synthase 2 antibody
    • Prostaglandin G/H synthase 2 precursor antibody
    • Prostaglandin G/H synthase and cyclooxygenase antibody
    • Prostaglandin G/H synthase antibody
    • Prostaglandin H2 synthase 2 antibody
    • prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase) antibody
    • Prostaglandin-endoperoxide synthase 2 antibody
    • PTGS2 antibody
    • ptgs2a antibody
    • TIS10 antibody
    • TIS10 protein antibody
    • unp1239 antibody
    • wu:fj02a10 antibody
    see all

Images

  • Western blot - Anti-COX2 / Cyclooxygenase 2 antibody [EPR12012] (ab179800)
    Western blot - Anti-COX2 / Cyclooxygenase 2 antibody [EPR12012] (ab179800)
    All lanes : Anti-COX2 / Cyclooxygenase 2 antibody [EPR12012] (ab179800) at 1/1000 dilution

    Lane 1 : B16-F10 (Mouse skin melanoma) whole cell lysate
    Lane 2 : Raw 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate
    Lane 3 : Mouse retina tissue lysate
    Lane 4 : Mouse hippocampus tissue lysate
    Lane 5 : Mouse heart tissue lysate
    Lane 6 : Mouse kidney tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 69 kDa
    Observed band size: 72 kDa why is the actual band size different from the predicted?


    Exposure time: 60 seconds


    Blocking buffer and concentration: 5% NFDM/TBST 

    Diluting buffer and concentration: 5% NFDM/TBST

    COX2 is expressed at a low level in Raw264.7, mouse retina, hippocampus, heart, kidney etc. (PMID: 22015457, PMID: 26001832, PMID: 23045674, PMID: 33737575).

  • Western blot - Anti-COX2 / Cyclooxygenase 2 antibody [EPR12012] (ab179800)
    Western blot - Anti-COX2 / Cyclooxygenase 2 antibody [EPR12012] (ab179800)
    All lanes : Anti-COX2 / Cyclooxygenase 2 antibody [EPR12012] (ab179800) at 1/1000 dilution

    Lane 1 : PTGS2 (COX2 / Cyclooxygenase 2) KO A549 (Human lung carcinoma epithelial cell) cell lysate with Intercept® (TBS) Blocking Buffer diluted with an equal volume of 0.1% TBS
    Lane 2 : Wild-type A549 cell lysate with Intercept® (TBS) Blocking Buffer diluted with an equal volume of 0.1% TBS
    Lane 3 : U-87 MG (Human glioblastoma-astrocytoma epithelial cell) whole cell lysate with Intercept® (TBS) Blocking Buffer diluted with an equal volume of 0.1% TBS
    Lane 4 : MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysate with Intercept® (TBS) Blocking Buffer diluted with an equal volume of 0.1% TBS

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/100000 dilution

    Performed under reducing conditions.

    Predicted band size: 69 kDa
    Observed band size: 74 kDa why is the actual band size different from the predicted?



    Negative control: MCF7 (PMID: 24325753, PMID: 16997132)

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    False colour image of Western blot: Anti- COX2 / Cyclooxygenase 2 antibody [EPR12012] (ab179800) staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red.

    In Western blot, ab179800 was shown to bind specifically to COX2 / Cyclooxygenase 2. Target band was observed at 74 kDa in wild-type A549 cell lysates with no signal observed at this size in COX2 / Cyclooxygenase 2 knockout cell line ab280802. To generate this image, wild-type and COX2 / Cyclooxygenase 2 knockout A549 cell lysates were analyzed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.

  • Western blot - Anti-COX2 / Cyclooxygenase 2 antibody [EPR12012] (ab179800)
    Western blot - Anti-COX2 / Cyclooxygenase 2 antibody [EPR12012] (ab179800)
    All lanes : Anti-COX2 / Cyclooxygenase 2 antibody [EPR12012] (ab179800) at 1/1000 dilution

    Lane 1 : Wild-type A549 cell lysate
    Lane 2 : PTGS2 knockout A549 cell lysate
    Lane 3 : U-87 MG cell lysate
    Lane 4 : MOLT-4 cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 69 kDa



    False colour image of Western blot: Anti-COX2 / Cyclooxygenase 2 antibody [EPR12012] staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab179800 was shown to bind specifically to COX2 / Cyclooxygenase 2. A band was observed at 75 kDa in wild-type A549 cell lysates with no signal observed at this size in PTGS2 knockout cell line ab280802 (knockout cell lysate ab283825). To generate this image, wild-type and PTGS2 knockout A549 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in fluorescent western blot (TBS-based) blocking solution before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.

  • Western blot - Anti-COX2 / Cyclooxygenase 2 antibody [EPR12012] (ab179800)
    Western blot - Anti-COX2 / Cyclooxygenase 2 antibody [EPR12012] (ab179800)
    All lanes : Anti-COX2 / Cyclooxygenase 2 antibody [EPR12012] (ab179800) at 1/1000 dilution

    Lane 1 : A549 cell lysate
    Lane 2 : U-87 MG cell lysate
    Lane 3 : Wild-type HeLa cell lysate
    Lane 4 : PTGS2 knockout HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 69 kDa



    Lanes 1 - 4: Merged signal (red and green). Green - ab179800 observed at 75 kDa. Red - loading control, ab8245 observed at 37 kDa.

     ab179800 was shown to react with COX2 / Cyclooxygenase 2 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab255420 (knockout cell lysate ab263795) was used. Wild-type and COX2 / Cyclooxygenase 2 knockout samples were subjected to SDS-PAGE. ab179800 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX2 / Cyclooxygenase 2 antibody [EPR12012] (ab179800)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX2 / Cyclooxygenase 2 antibody [EPR12012] (ab179800)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse kidney tissue sections labeling COX2 / Cyclooxygenase 2 with purified ab179800 at 1/4000 dilution (0.125 µg/ml).
    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: secondary antibody only control. Hematoxylin was used as a counterstain.
  • Immunocytochemistry/ Immunofluorescence - Anti-COX2 / Cyclooxygenase 2 antibody [EPR12012] (ab179800)
    Immunocytochemistry/ Immunofluorescence - Anti-COX2 / Cyclooxygenase 2 antibody [EPR12012] (ab179800)

    Immunocytochemistry/ Immunofluorescence analysis of U-87 MG (human glioblastoma-astrocytoma epithelial cell) cells labeling COX2 / Cyclooxygenase 2 with ab179800 at 1/50 dilution. ab150077 (AlexaFluor®488 Goat anti-Rabbit) at 1/1000 was used as secondary antibody. Cells were fixed with 4% Paraformaldehyde and permeabilised with 0.1% TritonX-100. ab195889, Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 1/200 was used as counterstain. Nuclie were stained blue with DAPI.

    Confocal image showing cytoplasmic staining in U-87 MG cell line.
    Negative control: MCF7 (PMID: 18199541)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX2 / Cyclooxygenase 2 antibody [EPR12012] (ab179800)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX2 / Cyclooxygenase 2 antibody [EPR12012] (ab179800)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse liver tissue sections labeling COX2 / Cyclooxygenase 2 with purified ab179800 at 1/4000 dilution (0.125 µg/ml).
    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: secondary antibody only control. Hematoxylin was used as a counterstain.
  • Western blot - Anti-COX2 / Cyclooxygenase 2 antibody [EPR12012] (ab179800)
    Western blot - Anti-COX2 / Cyclooxygenase 2 antibody [EPR12012] (ab179800)
    All lanes : Anti-COX2 / Cyclooxygenase 2 antibody [EPR12012] (ab179800) at 1/1000 dilution (Purified)

    Lane 1 : U-87 MG (human glioblastoma-astrocytoma epithelial cell line) whole cell lysate with 5% NFDM/TBST
    Lane 2 : HCT 116 (human colorectal carcinoma cell line) whole cell lysate with 5% NFDM/TBST
    Lane 3 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate with 5% NFDM/TBST

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 69 kDa
    Observed band size: 72 kDa why is the actual band size different from the predicted?



    Exposure time

    Lane 1: 3.25 seconds
    Lane 2 and 3: 180 seconds

     

    The expression profile observed in HCT 116 and MCF7 are consistent with the literatures (PMID: 14739610, PMID: 24325753, PMID: 16997132).
    Negative control: HCT 116 (PMID: 14739610) and MCF7 (PMID: 24325753, PMID: 16997132)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX2 / Cyclooxygenase 2 antibody [EPR12012] (ab179800)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX2 / Cyclooxygenase 2 antibody [EPR12012] (ab179800)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colonic carcinoma tissue labelling COX2 / Cyclooxygenase 2 with unpurified ab179800 at a dilution of 1/250.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX2 / Cyclooxygenase 2 antibody [EPR12012] (ab179800)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX2 / Cyclooxygenase 2 antibody [EPR12012] (ab179800)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded) analysis of human colon tissue labeling COX2 / Cyclooxygenase 2 with unpurified ab179800 at a dilution of 1/250.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX2 / Cyclooxygenase 2 antibody [EPR12012] (ab179800)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX2 / Cyclooxygenase 2 antibody [EPR12012] (ab179800)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded) analysis of human liver tissue labelling COX2 / Cyclooxygenase 2 with unpurified ab179800 at a dilution of 1/250.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Western blot - Anti-COX2 / Cyclooxygenase 2 antibody [EPR12012] (ab179800)
    Western blot - Anti-COX2 / Cyclooxygenase 2 antibody [EPR12012] (ab179800)
    Anti-COX2 / Cyclooxygenase 2 antibody [EPR12012] (ab179800) at 1/5000 dilution (purified) + Mouse spleen tissue lysate at 20 µg

    Secondary
    HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG at 1/50000 dilution

    Predicted band size: 69 kDa
    Observed band size: 72 kDa why is the actual band size different from the predicted?



    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-COX2 / Cyclooxygenase 2 antibody [EPR12012] (ab179800)
    Western blot - Anti-COX2 / Cyclooxygenase 2 antibody [EPR12012] (ab179800)
    Anti-COX2 / Cyclooxygenase 2 antibody [EPR12012] (ab179800) at 1/5000 dilution (purified) + A549 whole cell lysate at 20 µg

    Secondary
    HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG at 1/50000 dilution

    Predicted band size: 69 kDa
    Observed band size: 72 kDa why is the actual band size different from the predicted?



    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX2 / Cyclooxygenase 2 antibody [EPR12012] (ab179800)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX2 / Cyclooxygenase 2 antibody [EPR12012] (ab179800)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat kidney tissue labelling COX2 / Cyclooxygenase 2 with purified ab179800 at 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX2 / Cyclooxygenase 2 antibody [EPR12012] (ab179800)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX2 / Cyclooxygenase 2 antibody [EPR12012] (ab179800)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma tissue labelling COX2 / Cyclooxygenase 2 with purified ab179800 at 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

  • Immunoprecipitation - Anti-COX2 / Cyclooxygenase 2 antibody [EPR12012] (ab179800)
    Immunoprecipitation - Anti-COX2 / Cyclooxygenase 2 antibody [EPR12012] (ab179800)

    ab179800 (purified) at 1/30 immunoprecipitating COX2 in A549 whole cell lysate.

    Lane 1 (input): A549 whole cell lysate (10µg)

    Lane 2 (+): ab179800 + A549 whole cell lysate.

    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab179800 in A549 whole cell lysate.

    For western blotting, HRP-conjugated anti-rabbit IgG, specific for the reduced form of IgG, was used as the secondary antibody (1/1500).

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • Western blot - Anti-COX2 / Cyclooxygenase 2 antibody [EPR12012] (ab179800)
    Western blot - Anti-COX2 / Cyclooxygenase 2 antibody [EPR12012] (ab179800)
    Anti-COX2 / Cyclooxygenase 2 antibody [EPR12012] (ab179800) at 1/1000 dilution (unpurified) + A549 cell lysate at 10 µg

    Predicted band size: 69 kDa

  • Immunoprecipitation - Anti-COX2 / Cyclooxygenase 2 antibody [EPR12012] (ab179800)
    Immunoprecipitation - Anti-COX2 / Cyclooxygenase 2 antibody [EPR12012] (ab179800)

    Western blot analysis on immunoprecipitation pellet from A549 cell lysate using unpurified ab179800.

  • Anti-COX2 / Cyclooxygenase 2 antibody [EPR12012] (ab179800)
    Anti-COX2 / Cyclooxygenase 2 antibody [EPR12012] (ab179800)

Protocols

  • Western blot protocols
  • Immunoprecipitation protocols
  • Immunohistochemistry protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (39)

Publishing research using ab179800? Please let us know so that we can cite the reference in this datasheet.

ab179800 has been referenced in 39 publications.

  • Rui Q  et al. LMTK2 regulates inflammation in lipopolysaccharide-stimulated BV2 cells. Exp Ther Med 21:219 (2021). PubMed: 33603828
  • Niu D  et al. Protective Effects of Iridoid Glycoside from Corni Fructus on Type 2 Diabetes with Nonalcoholic Fatty Liver in Mice. Biomed Res Int 2021:3642463 (2021). PubMed: 33542919
  • Zhou H  et al. Microbial Metabolite Sodium Butyrate Attenuates Cartilage Degradation by Restoring Impaired Autophagy and Autophagic Flux in Osteoarthritis Development. Front Pharmacol 12:659597 (2021). PubMed: 33897442
  • Li M  et al. The Arachidonic Acid Metabolism Mechanism Based on UPLC-MS/MS Metabolomics in Recurrent Spontaneous Abortion Rats. Front Endocrinol (Lausanne) 12:652807 (2021). PubMed: 33868179
  • Sun M  et al. Ginkgolide B alleviates the inflammatory response and attenuates the activation of LPS-induced BV2 cells in vitro and in vivo. Exp Ther Med 21:586 (2021). PubMed: 33850558
View all Publications for this product

Customer reviews and Q&As

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1-3 of 3 Abreviews or Q&A

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-COX2 / Cyclooxygenase 2 antibody [EPR12012]

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rhesus monkey Tissue sections (Brain)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris/EDTA
Permeabilization
No
Specification
Brain
Blocking step
Vector Laboratories Supplier Diversity Partner VECTASTAIN Elite ABC HRP Kit as blocking agent for 30 minute(s) · Concentration: 10µg/mL
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Sudeshna Tripathy

Verified customer

Submitted Sep 26 2022

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-COX2 / Cyclooxygenase 2 antibody [EPR12012]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rhesus monkey Tissue sections (vagina)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: EDTA pH 9.0
Permeabilization
No
Specification
vagina
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2.5% · Temperature: 23°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Mar 18 2021

Western blot abreview for Anti-COX2 / Cyclooxygenase 2 antibody [EPR12012]

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Rat Tissue lysate - whole (Liver)
Gel Running Conditions
Reduced Denaturing (10% gel)
Loading amount
20 µg
Specification
Liver
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jun 12 2015

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