Recombinant
RabMAb

Recombinant Anti-COX2 / Cyclooxygenase 2 antibody [EPR12012] - BSA and Azide free (ab227528)

Overview

  • Product name

    Anti-COX2 / Cyclooxygenase 2 antibody [EPR12012] - BSA and Azide free
    See all COX2 / Cyclooxygenase 2 primary antibodies
  • Description

    Rabbit monoclonal [EPR12012] to COX2 / Cyclooxygenase 2 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, IP, ICC/IFmore details
    Unsuitable for: Flow Cyt
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human COX2 aa 550 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
    Database link: P35354

  • Positive control

    • WB: A549 cell lysate and mouse spleen tissue lysate. IHC-P: Human colonic carcinoma, lung carcinoma, liver and colon tissues and rat kidney tissue lysate. IP: A549 cell lysate.
  • General notes

    Ab227528 is the carrier-free version of ab179800. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab227528 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.??

Applications

Our Abpromise guarantee covers the use of ab227528 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 69 kDa.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

IP Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for Flow Cyt.
  • Target

    • Function

      Mediates the formation of prostaglandins from arachidonate. May have a role as a major mediator of inflammation and/or a role for prostanoid signaling in activity-dependent plasticity.
    • Pathway

      Lipid metabolism; prostaglandin biosynthesis.
    • Sequence similarities

      Belongs to the prostaglandin G/H synthase family.
      Contains 1 EGF-like domain.
    • Post-translational
      modifications

      S-nitrosylation by NOS2 (iNOS) activates enzme activity. S-nitrosylation may take place on different Cys residues in addition to Cys-561.
    • Cellular localization

      Microsome membrane. Endoplasmic reticulum membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • COX 2 antibody
      • COX-2 antibody
      • COX2 antibody
      • Cyclooxygenase 2 antibody
      • Cyclooxygenase 2b antibody
      • Cyclooxygenase antibody
      • Cyclooxygenase-2 antibody
      • Cyclooxygenase2 antibody
      • EC 1.14.99.1 antibody
      • fj02a10 antibody
      • Glucocorticoid-regulated inflammatory cyclooxygenase antibody
      • Glucocorticoid-regulated inflammatory Prostaglandin G/H synthase antibody
      • GRIPGHS antibody
      • hCox 2 antibody
      • Macrophage activation-associated marker protein P71/73 antibody
      • OTTHUMP00000033524 antibody
      • PES-2 antibody
      • PGG/HS antibody
      • PGH synthase 2 antibody
      • PGH2_HUMAN antibody
      • PGHS 2 antibody
      • PGHS-2 antibody
      • PGHS2 antibody
      • PHS 2 antibody
      • PHS II antibody
      • PHS2 antibody
      • Prostaglandin endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase) antibody
      • Prostaglandin endoperoxide synthase 2 antibody
      • Prostaglandin G/H synthase 2 antibody
      • Prostaglandin G/H synthase 2 precursor antibody
      • Prostaglandin G/H synthase and cyclooxygenase antibody
      • Prostaglandin G/H synthase antibody
      • Prostaglandin H2 synthase 2 antibody
      • prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase) antibody
      • Prostaglandin-endoperoxide synthase 2 antibody
      • PTGS2 antibody
      • ptgs2a antibody
      • TIS10 antibody
      • TIS10 protein antibody
      • unp1239 antibody
      • wu:fj02a10 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat kidney tissue labelling COX2 / Cyclooxygenase 2 with purified ab179800 at 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab179800).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma tissue labelling COX2 / Cyclooxygenase 2 with purified ab179800 at 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab179800).

    • ab179800 (purified) at 1/30 immunoprecipitating COX2 in A549 whole cell lysate.

      Lane 1 (input): A549 whole cell lysate (10µg)

      Lane 2 (+): ab179800 + A549 whole cell lysate.

      Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab179800 in A549 whole cell lysate.

      For western blotting, HRP-conjugated anti-rabbit IgG, specific for the reduced form of IgG, was used as the secondary antibody (1/1500).

      Blocking buffer and concentration: 5% NFDM/TBST.

      Diluting buffer and concentration: 5% NFDM /TBST.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab179800).

    • Western blot analysis on immunoprecipitation pellet from A549 cell lysate using unpurified ab179800.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab179800).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded) analysis of human colon tissue labeling COX2 / Cyclooxygenase 2 with unpurified ab179800 at a dilution of 1/250.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab179800).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded) analysis of human liver tissue labelling COX2 / Cyclooxygenase 2 with unpurified ab179800 at a dilution of 1/250.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab179800).

    • This IHC data was generated using the same anti-COX2 / Cyclooxygenase 2 antibody clone, EPR12012, in a different buffer formulation (cat# ab179800).

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colonic carcinoma tissue labelling COX2 with unpurified ab179800 at a dilution of 1/250.

    References

    This product has been referenced in:

    • Bu LJ  et al. Melatonin, a novel selective ATF-6 inhibitor, induces human hepatoma cell apoptosis through COX-2 downregulation. World J Gastroenterol 23:986-998 (2017). Read more (PubMed: 28246472) »
    • Xu K  et al. Autophagy attenuates the catabolic effect during inflammatory conditions in nucleus pulposus cells, as sustained by NF-?B and JNK inhibition. Int J Mol Med 36:661-8 (2015). Read more (PubMed: 26165348) »
    See all 2 Publications for this product

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