Recombinant
RabMAb

Recombinant Anti-COX2 / Cyclooxygenase 2 antibody [EPR468(2)] (ab133466)

Overview

  • Product name

    Anti-COX2 / Cyclooxygenase 2 antibody [EPR468(2)]
    See all COX2 / Cyclooxygenase 2 primary antibodies
  • Description

    Rabbit monoclonal [EPR468(2)] to COX2 / Cyclooxygenase 2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
    Unsuitable for: Flow Cyt,ICC or IP
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    A synthetic peptide corresponding to residues in Human COX2/ Cyclooxygenase 2 (UniProt ID: P35354).

  • General notes

    A trial size is available to purchase for this antibody.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Applications

Our Abpromise guarantee covers the use of ab133466 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/1000 - 1/10000. Predicted molecular weight: 69 kDa.
  • Application notes
    Is unsuitable for Flow Cyt,ICC or IP.
  • Target

    • Function

      Mediates the formation of prostaglandins from arachidonate. May have a role as a major mediator of inflammation and/or a role for prostanoid signaling in activity-dependent plasticity.
    • Pathway

      Lipid metabolism; prostaglandin biosynthesis.
    • Sequence similarities

      Belongs to the prostaglandin G/H synthase family.
      Contains 1 EGF-like domain.
    • Post-translational
      modifications

      S-nitrosylation by NOS2 (iNOS) activates enzme activity. S-nitrosylation may take place on different Cys residues in addition to Cys-561.
    • Cellular localization

      Microsome membrane. Endoplasmic reticulum membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • COX 2 antibody
      • COX-2 antibody
      • COX2 antibody
      • Cyclooxygenase 2 antibody
      • Cyclooxygenase 2b antibody
      • Cyclooxygenase antibody
      • Cyclooxygenase-2 antibody
      • Cyclooxygenase2 antibody
      • EC 1.14.99.1 antibody
      • fj02a10 antibody
      • Glucocorticoid-regulated inflammatory cyclooxygenase antibody
      • Glucocorticoid-regulated inflammatory Prostaglandin G/H synthase antibody
      • GRIPGHS antibody
      • hCox 2 antibody
      • Macrophage activation-associated marker protein P71/73 antibody
      • OTTHUMP00000033524 antibody
      • PES-2 antibody
      • PGG/HS antibody
      • PGH synthase 2 antibody
      • PGH2_HUMAN antibody
      • PGHS 2 antibody
      • PGHS-2 antibody
      • PGHS2 antibody
      • PHS 2 antibody
      • PHS II antibody
      • PHS2 antibody
      • Prostaglandin endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase) antibody
      • Prostaglandin endoperoxide synthase 2 antibody
      • Prostaglandin G/H synthase 2 antibody
      • Prostaglandin G/H synthase 2 precursor antibody
      • Prostaglandin G/H synthase and cyclooxygenase antibody
      • Prostaglandin G/H synthase antibody
      • Prostaglandin H2 synthase 2 antibody
      • prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase) antibody
      • Prostaglandin-endoperoxide synthase 2 antibody
      • PTGS2 antibody
      • ptgs2a antibody
      • TIS10 antibody
      • TIS10 protein antibody
      • unp1239 antibody
      • wu:fj02a10 antibody
      see all

    Images

    • All lanes : Anti-COX2 / Cyclooxygenase 2 antibody [EPR468(2)] (ab133466) at 1/1000 dilution

      Lane 1 : A549 treated with TNF-alpha and TPA
      Lane 2 : A549 cell lysate

      Predicted band size: 69 kDa



      Western blot analysis on (A) A549 treated with TNF-alpha and TPA and (B) A549 cell lysates using anti-COX2 RabMAb at dilution 1/1000

    References

    ab133466 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    GOAT BLOCK = 10mM Tris pH 7.4, 100mM MgCl2, 0.5% Tween, 1% BSA, 5% normal goat serum (IN WATER) as blocking agent for 20 minute(s) · Concentration: 100% · Temperature: 25°C
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Sodium Citrate
    Sample
    Mouse Tissue sections (pancreas)
    Specification
    pancreas
    Permeabilization
    No
    Fixative
    Formaldehyde

    Abcam user community

    Verified customer

    Submitted Jun 07 2013

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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