For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Customized Products & Partnerships
    Customized Products & Partnerships

    Customized products and commercial partnerships to accelerate your diagnostic and therapeutic programs.

    Customized products

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

  1. Link

    cox2--cyclooxygenase-2-antibody-sp21-ab16701.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Cardiovascular Blood Platelets
Share by email
RabMAb

Anti-COX2 / Cyclooxygenase 2 antibody [SP21] (ab16701)

  • Datasheet
  • SDS
Submit a review Q&A (4)References (6)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Shipping info

Promotion Information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX2 / Cyclooxygenase 2 antibody [SP21] (ab16701)

    Key features and details

    • Rabbit monoclonal [SP21] to COX2 / Cyclooxygenase 2
    • Suitable for: IHC-P
    • Reacts with: Human
    • Isotype: IgG

    You may also be interested in

    Primary
    Product image
    Anti-Prostaglandin E Synthase/MPGES-1 antibody [EPR13765] (ab180589)
    Protein
    Product image
    Recombinant human COX2 / Cyclooxygenase 2 protein (ab198646)
    Assay
    Product image
    Cyclooxygenase (COX) Activity Assay Kit (Fluorometric) (ab204699)

    View more associated products

    Overview

    • Product name

      Anti-COX2 / Cyclooxygenase 2 antibody [SP21]
      See all COX2 / Cyclooxygenase 2 primary antibodies
    • Description

      Rabbit monoclonal [SP21] to COX2 / Cyclooxygenase 2
    • Host species

      Rabbit
    • Tested applications

      Suitable for: IHC-Pmore details
      Unsuitable for: WB
    • Species reactivity

      Reacts with: Human
      Predicted to work with: Mouse, Rat
    • Immunogen

      Synthetic peptide within Rat COX2/ Cyclooxygenase 2 (C terminal). The exact sequence is proprietary.

    • Positive control

      • Lung carcinoma, colon carcinoma and breast carcinoma; HT 29 lysate.
    • General notes

      This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
    • Storage buffer

      pH: 7.50
      Preservative: 0.1% Sodium azide
      Constituents: Tissue culture supernatant, Tris buffered saline, 1% BSA
    • Concentration information loading...
    • Purity

      Tissue culture supernatant
    • Clonality

      Monoclonal
    • Clone number

      SP21
    • Isotype

      IgG
    • Research areas

      • Cardiovascular
      • Blood
      • Platelets
      • Cardiovascular
      • Lipids / Lipoproteins
      • Lipid Metabolism
      • Cholesterol Metabolism
      • Signal Transduction
      • Signaling Pathway
      • Lipid Signaling
      • Prostaglandins
      • Cancer
      • Tumor biomarkers
      • Other
      • Cardiovascular
      • Atherosclerosis
      • Lipoprotein metabolism
      • Cancer
      • Cancer Metabolism
      • Metabolic signaling pathway
      • Hormone biosynthesis
      • Metabolism
      • Pathways and Processes
      • Metabolic signaling pathways
      • Lipid and lipoprotein metabolism
      • Cholesterol Metabolism
      • Metabolism
      • Pathways and Processes
      • Metabolic signaling pathways
      • Lipid and lipoprotein metabolism
      • Lipoprotein metabolism
      • Metabolism
      • Pathways and Processes
      • Endocrine metabolism
      • Hormone biosynthesis
      • Metabolism
      • Types of disease
      • Cancer
      • Metabolism
      • Types of disease
      • Heart disease
      • Neuroscience
      • Processes

    Associated products

    • Isotype control

      • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
    • Positive Controls

      • HT-29 whole cell lysate (ab3952)
    • Recombinant Protein

      • Recombinant human COX2 / Cyclooxygenase 2 protein (ab198646)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab16701 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    IHC-P
    1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
    Notes
    IHC-P
    1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
    Application notes
    Is unsuitable for WB.

    Target

    • Function

      Mediates the formation of prostaglandins from arachidonate. May have a role as a major mediator of inflammation and/or a role for prostanoid signaling in activity-dependent plasticity.
    • Pathway

      Lipid metabolism; prostaglandin biosynthesis.
    • Sequence similarities

      Belongs to the prostaglandin G/H synthase family.
      Contains 1 EGF-like domain.
    • Post-translational
      modifications

      S-nitrosylation by NOS2 (iNOS) activates enzme activity. S-nitrosylation may take place on different Cys residues in addition to Cys-561.
    • Cellular localization

      Microsome membrane. Endoplasmic reticulum membrane.
    • Target information above from: UniProt accession P35354 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 5743 Human
      • Entrez Gene: 19225 Mouse
      • Entrez Gene: 29527 Rat
      • Omim: 600262 Human
      • SwissProt: P35354 Human
      • SwissProt: Q05769 Mouse
      • SwissProt: P35355 Rat
      • Unigene: 196384 Human
      • Unigene: 292547 Mouse
      • Unigene: 44369 Rat
      see all
    • Alternative names

      • COX 2 antibody
      • COX-2 antibody
      • COX2 antibody
      • Cyclooxygenase 2 antibody
      • Cyclooxygenase 2b antibody
      • Cyclooxygenase antibody
      • Cyclooxygenase-2 antibody
      • Cyclooxygenase2 antibody
      • EC 1.14.99.1 antibody
      • fj02a10 antibody
      • Glucocorticoid-regulated inflammatory cyclooxygenase antibody
      • Glucocorticoid-regulated inflammatory Prostaglandin G/H synthase antibody
      • GRIPGHS antibody
      • hCox 2 antibody
      • Macrophage activation-associated marker protein P71/73 antibody
      • OTTHUMP00000033524 antibody
      • PES-2 antibody
      • PGG/HS antibody
      • PGH synthase 2 antibody
      • PGH2_HUMAN antibody
      • PGHS 2 antibody
      • PGHS-2 antibody
      • PGHS2 antibody
      • PHS 2 antibody
      • PHS II antibody
      • PHS2 antibody
      • Prostaglandin endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase) antibody
      • Prostaglandin endoperoxide synthase 2 antibody
      • Prostaglandin G/H synthase 2 antibody
      • Prostaglandin G/H synthase 2 precursor antibody
      • Prostaglandin G/H synthase and cyclooxygenase antibody
      • Prostaglandin G/H synthase antibody
      • Prostaglandin H2 synthase 2 antibody
      • prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase) antibody
      • Prostaglandin-endoperoxide synthase 2 antibody
      • PTGS2 antibody
      • ptgs2a antibody
      • TIS10 antibody
      • TIS10 protein antibody
      • unp1239 antibody
      • wu:fj02a10 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX2 / Cyclooxygenase 2 antibody [SP21] (ab16701)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX2 / Cyclooxygenase 2 antibody [SP21] (ab16701)
      ab16701 at 1/100 dilution staining COX2 / Cyclooxygenase 2 in human breast carcinoma by Immunohistochemistry (FFPE-sections).

    Protocols

    • Immunohistochemistry protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (6)

    Publishing research using ab16701? Please let us know so that we can cite the reference in this datasheet.

    ab16701 has been referenced in 6 publications.

    • Szlendak M  et al. Expression of cyclooxygenase-2 and mucin 1 in colorectal cancer. Mol Clin Oncol 13:52 (2020). PubMed: 32874582
    • Keren L  et al. MIBI-TOF: A multiplexed imaging platform relates cellular phenotypes and tissue structure. Sci Adv 5:eaax5851 (2019). Mass Cytometry . PubMed: 31633026
    • Peng YJ  et al. Adiponectin and adiponectin receptor 1 overexpression enhance inflammatory bowel disease. J Biomed Sci 25:24 (2018). PubMed: 29540173
    • Szylberg L  et al. Expression of COX-2, IL-1ß, TNF-a and IL-4 in epithelium of serrated adenoma, adenoma and hyperplastic polyp. Arch Med Sci 12:172-8 (2016). PubMed: 26925134
    • Fujita M  et al. COX-2 blockade suppresses gliomagenesis by inhibiting myeloid-derived suppressor cells. Cancer Res 71:2664-74 (2011). PubMed: 21324923
    • Lu TC  et al. Analgesic and Anti-Inflammatory Activities of the Methanol Extract from Pogostemon cablin. Evid Based Complement Alternat Med : (2009). PubMed: 19933324

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-4 of 4 Abreviews or Q&A

    Question

    I was wondering if you can provide us an information about concentration of that antibody in ug/ml (or in similar units) OR give us the information how much antibodies are in those 500 microliters in micrograms, milligrams or grams.

    Read More

    Abcam community

    Verified customer

    Asked on May 07 2013

    Answer

    Unfortunately it is not possible to determine the concentration since tissue culture supernatant will contain many other host proteins besides the antibody of interest as it is unpurified. As a rough guide tissue culture supernatant contains 1 mg/ml total antibodies and 0.05 mg/ml specific antibodies.

    Read More

    Abcam Scientific Support

    Answered on May 07 2013

    Question

    BATCH NUMBER 237901 ORDER NUMBER 196732 DESCRIPTION OF THE PROBLEM There are strong bands at the wrong band sizes (see attached file). SAMPLE Rat liver hepatoma, H4IIE-cells treated with TNF-alpha PRIMARY ANTIBODY abcam, ab16701-500 Rabbit monoclonal [SP21] to COX2/ Cyclooxygenase 2 diluted 1/200 in 1% milk powder in TBS-Tween incubation time: 2.5 hours at room temperature agitating wash step: 3 x 5 min with TBS-Tween DETECTION METHOD ECL, substrate and peroxide buffer from tebu-bio : Chemiluminescent Sensitive HRP Substrate Kit (SuperDetect);2-Component Chemiluminescent Substrate for the Detection of Horseradish Peroxidase. Detection with Lumi-Imager from Roche (Lumi-Analyst Software) or X-ray films. ANTIBODY STORAGE CONDITIONS +4°C in storage buffer (data sheet) SAMPLE PREPARATION H4IIE-cells were treated with different concentrations of TNF-alpha for 6 hours. Incubation scheme: 1 000 000 cells per 60 mm petri dish were sowed in DMEM low glucose containing 10% BSA and 1% P/S and left to adhere for 24 hours at 37 °C. Then the culture medium was evacuated and the cells were washed with 0,9 % sodium chloride. Then The cells were incubated with 10, 30, 50 or 70 ng/ml TNF-alpha (dissolved in culture medium) for 6 hours. After 6 hours the incubation medium was evacuated and the cells were washed three times with 0,9 % sodium chloride. Then the cells were incubated with culture medium for another 24 hours. After 24 hours the medium was evacuated and cells were washed with 0,9 % sodium chloride. Cell lysis has been carried out by harvesting the cells with 70 µl RIPA-Lysis-buffer per 60 mm dish (50 mM Tris/HCl pH 7.4, 150 mM NaCl, 1 mM PMSF, 1 mM EDTA, 1% Triton X-100, 1 % sodium deoxycholate, 0,1 % SDS, 50 µl Protease Inhibitor Cocktail from sigma). Cell suspension was then incubated for 15 min on ice with gentle agitation and centrifugated for 15 min with 14 000 x g at 4 °C. Supernatant was evacuated and stored at -80°C until protein determination by Bradford Assay.Samples were diluted with RIPA-buffer to 4 µg/µl total protein. From this solution 12 µl were diluted with 6 µl Laemmli buffer (3x) and heated up to 95 °C for 5-6 min. The samples were pulsed and loaded onto the gel. AMOUNT OF PROTEIN LOADED 48 µg total protein per lane, protein determined by Bradford Assay ELECTROPHORESIS/GEL CONDITIONS stacking: 4 % SDS-polyacrylamidgel separating/resolving: 10% SDS-polyacrylamidgel buffer: 2 M glycine, 250 mM Tris, 1 % SDS run: 70 V for 15 min, 100 V for 1 h 40 min TRANSFER AND BLOCKING CONDITIONS discontinuous buffer system: anode buffer I : 300 mM Tris, 10 % methanol, pH 10.4 anode buffer II: 25 mM Tris, 10 % methanol, pH 10.4 cathode buffer: 40 mM glycine, 25 mM Tris, 10 % methanol, SDS, pH 9.4 time period: 1 h 25 min at 90 mA per membrane PVDF membrane (Millipore) blocking conditions: 1 h at room temperature with 5 % milk powder in TBS wash step after blocking: 3 x 5 min with TBS-Tween SECONDARY ANTIBODY [another company]./ goat anti rabbit HRP-linked diluted 1/1000 in 1 % milk powder in TBS-Tween incubation time: 1.5 hours at room temperature agitating wash step: 3 x 5 min with TBS-Tween HOW MANY TIMES HAVE YOU TRIED THE APPLICATION? 3 DO YOU OBTAIN THE SAME RESULTS EVERY TIME? Yes WHAT STEPS HAVE YOU ALTERED? washing steps reduced, electrophoresis run extended, blotting time extended, new dilution of frozen samples, ADDITIONAL NOTES The attached file shows the last western blot. Loaded samples: H4IIE-Lysate (as described above) from left to right: 10, 30, 50, 70 ng/ml TNF-alpha incubation (6h); 48 µg total protein per lane. Two different times of exposition (30 sec and 300 sec) . Detected with chemiluminescence substrate from tebu-bio and Lumi-Imager (Roche).

    Read More

    Abcam community

    Verified customer

    Asked on Jan 08 2007

    Answer

    I'm very sorry to hear you are experiencing problems with ab16701. The protocol you have used is very good and should work well; in light of your problems it is clear that the antibody did not work well in western blotting. I would like to offer you a refund or credit note on your order, or another anti COX2 antibody of the same value as ab16701. Could you please tell me which you would prefer and I will arrange this immediately, I look forward to hearing from you to arrange this,

    Read More

    Abcam Scientific Support

    Answered on Jan 09 2007

    Question

    The catalog number I meant to inquire about is ab16701.

    Read More

    Abcam community

    Verified customer

    Asked on Oct 07 2005

    Answer

    Thank you for your enquiry. I am not sure that I follow your question, please can you elaborate. ab16701, anti-COX2 recognizes both the rat COX2 (69164KDa) and the human COX2 (68996KDa). I hope this information helps. Please do not hesitate to contact me should you require further assistance.

    Read More

    Abcam Scientific Support

    Answered on Oct 11 2005

    Question

    For WB with ab16701, what type of lystae is a good positive control?

    Read More

    Abcam community

    Verified customer

    Asked on Aug 26 2005

    Answer

    Our source for this antibody has recommended HT 29 lysate as a positive control for Western blotting. This type of lysate can be obtained from Abcam; it is catalog # ab3952. If you have any additional questions, please contact us again.

    Read More

    Abcam Scientific Support

    Answered on Aug 30 2005

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Get resources and offers direct to your inbox Sign up
    A-Z by research area
    • Cancer
    • Cardiovascular
    • Cell biology
    • Developmental biology
    • Epigenetics & Nuclear signaling
    • Immunology
    • Metabolism
    • Microbiology
    • Neuroscience
    • Signal transduction
    • Stem cells
    A-Z by product type
    • Primary antibodies
    • Secondary antibodies
    • Biochemicals
    • Isotype controls
    • Flow cytometry multi-color selector
    • Kits
    • Loading controls
    • Lysates
    • Peptides
    • Proteins
    • Slides
    • Tags and cell markers
    • Tools & Reagents
    Help & support
    • Support
    • Make an Inquiry
    • Protocols & troubleshooting
    • Placing an order
    • RabMAb products
    • Biochemical product FAQs
    • Training
    • Browse by Target
    Company
    • Corporate site
    • Investor relations
    • Company news
    • Careers
    • About us
    • Blog
    Events
    • Tradeshows
    • Conferences
    International websites
    • abcam.cn
    • abcam.co.jp

    Join with us

    • LinkedIn
    • facebook
    • Twitter
    • YouTube
    • Terms of sale
    • Website terms of use
    • Cookie policy
    • Privacy policy
    • Legal
    • Modern slavery statement
    © 1998-2022 Abcam plc. All rights reserved.