RabMAb

Anti-COX2 / Cyclooxygenase 2 antibody [SP21] (ab16701)

Submit an Abreview Q&A (4)Specific References (4)

Overview

  • Product name
    Anti-COX2 / Cyclooxygenase 2 antibody [SP21]
    See all COX2 / Cyclooxygenase 2 primary antibodies
  • Description
    Rabbit monoclonal [SP21] to COX2 / Cyclooxygenase 2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-Pmore details
    Unsuitable for: WB
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Positive control
    • Lung carcinoma, colon carcinoma and breast carcinoma; HT 29 lysate.

Applications

Our Abpromise guarantee covers the use of ab16701 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for WB.

    • Target

    • Function
      Mediates the formation of prostaglandins from arachidonate. May have a role as a major mediator of inflammation and/or a role for prostanoid signaling in activity-dependent plasticity.
    • Pathway
      Lipid metabolism; prostaglandin biosynthesis.
    • Sequence similarities
      Belongs to the prostaglandin G/H synthase family.
      Contains 1 EGF-like domain.
    • Post-translational
      modifications
      S-nitrosylation by NOS2 (iNOS) activates enzme activity. S-nitrosylation may take place on different Cys residues in addition to Cys-561.
    • Cellular localization
      Microsome membrane. Endoplasmic reticulum membrane.
    • Information by UniProt
    • Database links
      see all
    • Alternative names
      • COX 2 antibody
      • COX-2 antibody
      • COX2 antibody
      • Cyclooxygenase 2 antibody
      • Cyclooxygenase 2b antibody
      • Cyclooxygenase antibody
      • Cyclooxygenase-2 antibody
      • Cyclooxygenase2 antibody
      • EC 1.14.99.1 antibody
      • fj02a10 antibody
      • Glucocorticoid-regulated inflammatory cyclooxygenase antibody
      • Glucocorticoid-regulated inflammatory Prostaglandin G/H synthase antibody
      • GRIPGHS antibody
      • hCox 2 antibody
      • Macrophage activation-associated marker protein P71/73 antibody
      • OTTHUMP00000033524 antibody
      • PES-2 antibody
      • PGG/HS antibody
      • PGH synthase 2 antibody
      • PGH2_HUMAN antibody
      • PGHS 2 antibody
      • PGHS-2 antibody
      • PGHS2 antibody
      • PHS 2 antibody
      • PHS II antibody
      • PHS2 antibody
      • Prostaglandin endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase) antibody
      • Prostaglandin endoperoxide synthase 2 antibody
      • Prostaglandin G/H synthase 2 antibody
      • Prostaglandin G/H synthase 2 precursor antibody
      • Prostaglandin G/H synthase and cyclooxygenase antibody
      • Prostaglandin G/H synthase antibody
      • Prostaglandin H2 synthase 2 antibody
      • prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase) antibody
      • Prostaglandin-endoperoxide synthase 2 antibody
      • PTGS2 antibody
      • ptgs2a antibody
      • TIS10 antibody
      • TIS10 protein antibody
      • unp1239 antibody
      • wu:fj02a10 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX2 / Cyclooxygenase 2 antibody [SP21] (ab16701)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX2 / Cyclooxygenase 2 antibody [SP21] (ab16701)
      ab16701 at 1/100 dilution staining COX2 / Cyclooxygenase 2 in human breast carcinoma by Immunohistochemistry (FFPE-sections).

    References

    This product has been referenced in:
    • Peng YJ  et al. Adiponectin and adiponectin receptor 1 overexpression enhance inflammatory bowel disease. J Biomed Sci 25:24 (2018). Read more (PubMed: 29540173) »
    • Szylberg L  et al. Expression of COX-2, IL-1ß, TNF-a and IL-4 in epithelium of serrated adenoma, adenoma and hyperplastic polyp. Arch Med Sci 12:172-8 (2016). Read more (PubMed: 26925134) »

    See all 4 Publications for this product

    Publishing research using ab16701? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    4 reviews or Q&A

    10
    Votes: Highest First

    I was wondering if you can provide us an information about concentration of that antibody in ug/ml (or in similar units) OR give us the information how much antibodies are in those 500 microliters in micrograms, milligrams or grams.

    Unfortunately it is not possible to determine the concentration since tissue culture supernatant will contain many other host proteins besides the antibody of interest as it is unpurified. As a rough guide tissue culture supernatant contains 1 mg/ml to...

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    BATCH NUMBER 237901 ORDER NUMBER 196732 DESCRIPTION OF THE PROBLEM There are strong bands at the wrong band sizes (see attached file). SAMPLE Rat liver hepatoma, H4IIE-cells treated with TNF-alpha PRIMARY ANTIBODY abcam, ab16...

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    I'm very sorry to hear you are experiencing problems with ab16701. The protocol you have used is very good and should work well; in light of your problems it is clear that the antibody did not work well in western blotting. I would like to offer ...

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    The catalog number I meant to inquire about is ab16701.

    Thank you for your enquiry. I am not sure that I follow your question, please can you elaborate. ab16701, anti-COX2 recognizes both the rat COX2 (69164KDa) and the human COX2 (68996KDa). I hope this information helps. Please do not hesitate...

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    For WB with ab16701, what type of lystae is a good positive control?

    Our source for this antibody has recommended HT 29 lysate as a positive control for Western blotting. This type of lysate can be obtained from Abcam; it is catalog # ab3952. If you have any additional questions, please contact us again.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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