Anti-COX2 / Cyclooxygenase 2 antibody [5E10/D10] (ab701)
Key features and details
- Mouse monoclonal [5E10/D10] to COX2 / Cyclooxygenase 2
- Suitable for: WB
- Reacts with: Sheep
- Isotype: IgG1
Overview
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Product name
Anti-COX2 / Cyclooxygenase 2 antibody [5E10/D10]
See all COX2 / Cyclooxygenase 2 primary antibodies -
Description
Mouse monoclonal [5E10/D10] to COX2 / Cyclooxygenase 2 -
Host species
Mouse -
Specificity
COX-2. No cross-reactivity with Cox-1. -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Sheep -
Immunogen
Full length native protein (purified) corresponding to Human COX2/ Cyclooxygenase 2. COX-2 purified from HL-60 cells
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General notes
Please note that we have received a report from a customer working with this antibody that they obtain two stong bands in WB (one between 40 - 50kDa and other other between 62 - 83kDa). A single band at around 72kDa is expected.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.02% Sodium azide
FCS free -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Monoclonal -
Clone number
5E10/D10 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab701 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 0.5 - 1 µg/ml.
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Notes |
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WB
Use a concentration of 0.5 - 1 µg/ml. |
Target
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Function
Mediates the formation of prostaglandins from arachidonate. May have a role as a major mediator of inflammation and/or a role for prostanoid signaling in activity-dependent plasticity. -
Pathway
Lipid metabolism; prostaglandin biosynthesis. -
Sequence similarities
Belongs to the prostaglandin G/H synthase family.
Contains 1 EGF-like domain. -
Post-translational
modificationsS-nitrosylation by NOS2 (iNOS) activates enzme activity. S-nitrosylation may take place on different Cys residues in addition to Cys-561. -
Cellular localization
Microsome membrane. Endoplasmic reticulum membrane. - Information by UniProt
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Database links
- Entrez Gene: 443460 Sheep
- SwissProt: P79208 Sheep
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Alternative names
- COX 2 antibody
- COX-2 antibody
- COX2 antibody
see all
Images
Datasheets and documents
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Datasheet download
References (0)
ab701 has not yet been referenced specifically in any publications.