Overview

  • Product name
    Anti-COX2 / Cyclooxygenase 2 antibody
    See all COX2 / Cyclooxygenase 2 primary antibodies
  • Description
    Goat polyclonal to COX2 / Cyclooxygenase 2
  • Host species
    Goat
  • Specificity
    ab23672 recognises COX2. It does not cross react with COX1.
  • Tested applications
    Suitable for: WB, IHC-P, ICC, Flow Cyt, ICC/IF, IHC-FoFrmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Sheep, Human
    Predicted to work with: Horse, Cow, Dog, Pig, Mink
  • Immunogen

    Synthetic peptide:

    TINASSSRSGLDDINPTVL

    , corresponding to amino acids 578/596 of Human COX2/ Cyclooxygenase 2(Peptide available as ab49841.)

Properties

Applications

Our Abpromise guarantee covers the use of ab23672 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2 µg/ml. Predicted molecular weight: 69 kDa.Can be blocked with COX2 / Cyclooxygenase 2 peptide (ab49841).
IHC-P Use a concentration of 1 - 2 µg/ml. Do not perform antigen retrieval.
ICC Use a concentration of 1 - 2 µg/ml.
Flow Cyt 1/600.

needs permeabilization

 

 

 

ab37373 - Goat polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

ICC/IF Use at an assay dependent concentration.
IHC-FoFr 1/500. PubMed: 19632905

Target

  • Function
    Mediates the formation of prostaglandins from arachidonate. May have a role as a major mediator of inflammation and/or a role for prostanoid signaling in activity-dependent plasticity.
  • Pathway
    Lipid metabolism; prostaglandin biosynthesis.
  • Sequence similarities
    Belongs to the prostaglandin G/H synthase family.
    Contains 1 EGF-like domain.
  • Post-translational
    modifications
    S-nitrosylation by NOS2 (iNOS) activates enzme activity. S-nitrosylation may take place on different Cys residues in addition to Cys-561.
  • Cellular localization
    Microsome membrane. Endoplasmic reticulum membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • COX 2 antibody
    • COX-2 antibody
    • COX2 antibody
    • Cyclooxygenase 2 antibody
    • Cyclooxygenase 2b antibody
    • Cyclooxygenase antibody
    • Cyclooxygenase-2 antibody
    • Cyclooxygenase2 antibody
    • EC 1.14.99.1 antibody
    • fj02a10 antibody
    • Glucocorticoid-regulated inflammatory cyclooxygenase antibody
    • Glucocorticoid-regulated inflammatory Prostaglandin G/H synthase antibody
    • GRIPGHS antibody
    • hCox 2 antibody
    • Macrophage activation-associated marker protein P71/73 antibody
    • OTTHUMP00000033524 antibody
    • PES-2 antibody
    • PGG/HS antibody
    • PGH synthase 2 antibody
    • PGH2_HUMAN antibody
    • PGHS 2 antibody
    • PGHS-2 antibody
    • PGHS2 antibody
    • PHS 2 antibody
    • PHS II antibody
    • PHS2 antibody
    • Prostaglandin endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase) antibody
    • Prostaglandin endoperoxide synthase 2 antibody
    • Prostaglandin G/H synthase 2 antibody
    • Prostaglandin G/H synthase 2 precursor antibody
    • Prostaglandin G/H synthase and cyclooxygenase antibody
    • Prostaglandin G/H synthase antibody
    • Prostaglandin H2 synthase 2 antibody
    • prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase) antibody
    • Prostaglandin-endoperoxide synthase 2 antibody
    • PTGS2 antibody
    • ptgs2a antibody
    • TIS10 antibody
    • TIS10 protein antibody
    • unp1239 antibody
    • wu:fj02a10 antibody
    see all

Images

  • All lanes : Anti-COX2 / Cyclooxygenase 2 antibody (ab23672) at 1/500 dilution

    Lane 1 : Human COX-1
    Lane 2 : Recombinant ovine COX-2 protein
    Lane 3 : Recombinant human COX-2 protein

    Predicted band size: 69 kDa

  • ab23672 staining COX2/Cyclooygenase 2 in Human synovial fibroblasts by Flow Cytometry. Cells were detached with accutase, suspended in PBS + 1% BSA, fixed with formaldehyde and permeabilized with methanol for 15 nminutes at -20°C. The sample was incubated with the primary antibody (1/600 in PBS + 1% BSA) for 30 minutes at 22°C. A Biotin-conjugated Rabbit anti-goat polyclonal (1/144) was used as the secondary antibody.
    Gating Strategy: Fibroblasts.
    Legend : Red: isotype; Green: unstimulated; Black: TNF (10 ng/ml for 24 hours) stimulated.

    See Abreview

  • ab23672 at a 1/500 dilution staining COX2 / Cyclooxygenase 2 in human synovial fibroblasts by Immunocytochemistry/ Immunofluorescence.
    Cells were fixed in formaldehyde and permeabilized using 0.1% Triton X-100. 1% BSA was used to block samples. The secondary used was a Cy5® conjugated rabbit anti-goat at a 1/200 dilution.

References

This product has been referenced in:
  • Lv H  et al. The shear wave elastic modulus and the increased nuclear factor kappa B (NF-kB/p65) and cyclooxygenase-2 (COX-2) expression in the area of myofascial trigger points activated in a rat model by blunt trauma to the vastus medialis. J Biomech 66:44-50 (2018). WB ; Rat . Read more (PubMed: 29137729) »
  • Salker MS  et al. LEFTYA Activates the Epithelial Na+ Channel (ENaC) in Endometrial Cells via Serum and Glucocorticoid Inducible Kinase SGK1. Cell Physiol Biochem 39:1295-306 (2016). Read more (PubMed: 27606670) »
See all 7 Publications for this product

Customer reviews and Q&As

1-9 of 9 Abreviews or Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (peritoneal tumor)
Permeabilization
Yes - Triton-X
Specification
peritoneal tumor
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted May 24 2018

Application
Western blot
Sample
Human Tissue lysate - whole (Colon)
Gel Running Conditions
Reduced Denaturing (10)
Loading amount
20 µg
Specification
Colon
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Mar 15 2018

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Colon)
Permeabilization
Yes - Triton-x
Specification
Colon
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Mar 15 2018

Application
Western blot
Sample
Mouse Tissue lysate - whole (Small intestine)
Gel Running Conditions
Reduced Denaturing
Loading amount
30 µg
Specification
Small intestine
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Aug 21 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (Small intestine)
Loading amount
50 µg
Specification
Small intestine
Gel Running Conditions
Reduced Denaturing (12%)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Sep 10 2012

Answer

Thank you for your inquiry. You are correct we do not have antibodies against these targets that are gauranteed for rabbit and IHC-P. But I am happy to confirm that for COX2 / Cyclooxygenase 2 antibody and Anti-Prostaglandin E Receptor EP3 I found antibodies that are tested in IHC-P. The immunogens of these antibodies show a high homology with the tested species and rabbit. Therefore, I can offer a discount off a future purchase if your customer buys ab10940 (Anti-COX2 / Cyclooxygenase 2 antibody) and/or ab115667 (Anti-Prostaglandin E Receptor EP3 antibody)  now, test it in rabbit and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free primary antibody each. If your customer is interested in this offer, please follow these steps: 1. Reply to this e-mail to let me know that you would like to proceed and test ab10940 and/or ab115667   in (UNTESTED APP/SPECIES). I will then send a discount code. This code must be issued before purchasing ab10940 and/or ab115667 so please wait for my reply before ordering. 2. Purchase ab10940 and/or ab115667 either by phone, fax, or online (www.abcam.com). 3. Test it in rabbit. 4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews. 5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any primary antibody ordered and the discount code is valid for 4 months after issue. We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab10940 or ab115667  turns out to be unsuitable for rabbit, your customer will still receive the discount on your next purchase after your Abreview has been submitted. Please let me know if you have any questions about this offer and I would be happy to help you further. The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.  

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (synovial fibroblasts)
Specification
synovial fibroblasts
Fixative
Formaldehyde
Permeabilization
Yes - 0,1% TritonX-100 in PBS
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 21°C

Dr. Torsten Lowin

Verified customer

Submitted Dec 14 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Flow Cytometry
Sample
Human Cell (synovial fibroblasts)
Specification
synovial fibroblasts
Preparation
Cell harvesting/tissue preparation method: cells detached with accutase (PAA)
Sample buffer: PBS with 1%BSA
Fixation
Formaldehyde
Permeabilization
Yes - methanol, 15min -20 degrees
Gating Strategy
only one gate: fibroblasts

Dr. Torsten Lowin

Verified customer

Submitted Nov 19 2010

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (adult brain hippocampus)
Specification
adult brain hippocampus
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid pH6
Permeabilization
No
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: RT°C

Mr. Carl Hobbs

Verified customer

Submitted Feb 29 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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