Anti-COX20 antibody - C-terminal (ab224570)
Key features and details
- Rabbit polyclonal to COX20 - C-terminal
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-COX20 antibody - C-terminal -
Description
Rabbit polyclonal to COX20 - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human COX20 aa 50 to the C-terminus (C terminal).
Database link: Q5RI15 -
Positive control
- IHC-P: Human kidney tissue. ICC/IF: U-2 OS cells. WB: A-431 and HEK 293 cell lines.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab224570 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100. |
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WB |
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 13 kDa.
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IHC-P |
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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ICC/IF
Use a concentration of 0.25 - 2 µg/ml. Fixation/Permeabilization: PFA/Triton X-100. |
WB
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 13 kDa. |
IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Relevance
COX20 is a multi-pass membrane protein. It belongs to the COX20 family. The exact function of COX20 remains unknown. -
Cellular localization
Membrane; Multi-pass membrane protein. -
Database links
- Entrez Gene: 116228 Human
- SwissProt: Q5RI15 Human
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Alternative names
- COX20 antibody
- COX20 cytochrome c oxidase assembly factor antibody
- Cytochrome c oxidase protein 20 homolog antibody
see all
Images
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All lanes : Anti-COX20 antibody - C-terminal (ab224570) at 0.4 µg/ml
Lane 1 : A-431 cell line
Lane 2 : HEK 293 cell line
Predicted band size: 13 kDa -
PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for COX20 (green) using ab224570 at 4 µg/ml in ICC/IF.
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Paraffin-embedded human kidney tissue stained for COX20 with ab224570 at 1/50 dilution in immunohistochemical analysis.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab224570 has been referenced in 1 publication.
- Li P et al. Compound Heterozygous COX20 Variants Impair the Function of Mitochondrial Complex IV to Cause a Syndrome Involving Ophthalmoplegia and Visual Failure. Front Neurol 13:873943 (2022). PubMed: 35651336