Overview

  • Product name

    Anti-COX20 antibody - C-terminal
    See all COX20 primary antibodies
  • Description

    Rabbit polyclonal to COX20 - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human COX20 aa 79-118 (C terminal).
    Sequence:

    YNYAKQRIQERIAREEIKKKILYEGTHLDPERKHNGSSSN


    Database link: Q5RI15

  • Positive control

    • IHC-P: Human kidney tissue. ICC/IF: U-2 OS cells. WB: RT4 and U-251 MG sp cell lysate; Human liver lysate.
  • General notes

    Previously labelled as FAM36A. 

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 40% Glycerol, PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab224570 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Fixation/Permeabilization: PFA/Triton X-100.

WB 1/100 - 1/250. Predicted molecular weight: 13 kDa.
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Relevance

    COX20 is a multi-pass membrane protein. It belongs to the COX20 family. The exact function of COX20 remains unknown.
  • Cellular localization

    Membrane; Multi-pass membrane protein.
  • Database links

  • Alternative names

    • COX20 antibody
    • COX20 cytochrome c oxidase assembly factor antibody
    • Cytochrome c oxidase protein 20 homolog antibody
    • FAM 36A antibody
    • FAM36A antibody
    • Family with sequence similarity 36 member A antibody
    • FLJ43269 antibody
    • Hypothetical protein LOC116228 antibody
    • OTTHUMP00000038200 antibody
    • Protein FAM36A antibody
    see all

Images

  • PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for COX20 (green) using ab224570 at 4 µg/ml in ICC/IF.

  • Paraffin-embedded human kidney tissue stained for COX20 with ab224570 at 1/50 dilution in immunohistochemical analysis.

  • All lanes : Anti-COX20 antibody - C-terminal (ab224570) at 1/100 dilution

    Lane 1 : RT4 (human urinary bladder cancer cell line) cell lysate
    Lane 2 : U-251 MG sp cell lysate
    Lane 3 : Human plasma (IgG/HSA depleted)
    Lane 4 : Human liver lysate

    Developed using the ECL technique.

    Predicted band size: 13 kDa

References

ab224570 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab224570.
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