Key features and details
- Rabbit polyclonal to COX20 - C-terminal
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-COX20 antibody - C-terminal
DescriptionRabbit polyclonal to COX20 - C-terminal
Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
Species reactivityReacts with: Human
- IHC-P: Human kidney tissue. ICC/IF: U-2 OS cells. WB: RT4 and U-251 MG sp cell lysate; Human liver lysate.
Previously labelled as FAM36A.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab224570 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100.
|WB||Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 13 kDa.|
|IHC-P||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
RelevanceCOX20 is a multi-pass membrane protein. It belongs to the COX20 family. The exact function of COX20 remains unknown.
Cellular localizationMembrane; Multi-pass membrane protein.
- COX20 antibody
- COX20 cytochrome c oxidase assembly factor antibody
- Cytochrome c oxidase protein 20 homolog antibody
PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for COX20 (green) using ab224570 at 4 µg/ml in ICC/IF.
Paraffin-embedded human kidney tissue stained for COX20 with ab224570 at 1/50 dilution in immunohistochemical analysis.
All lanes : Anti-COX20 antibody - C-terminal (ab224570) at 1/100 dilution
Lane 1 : RT4 (human urinary bladder cancer cell line) cell lysate
Lane 2 : U-251 MG sp cell lysate
Lane 3 : Human plasma (IgG/HSA depleted)
Lane 4 : Human liver lysate
Developed using the ECL technique.
Predicted band size: 13 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab224570 has not yet been referenced specifically in any publications.