Overview

  • Product name

    Anti-COX4I2 antibody
  • Description

    Rabbit polyclonal to COX4I2
  • Host species

    Rabbit
  • Specificity

    ab70112 detects endogenous levels of total COX4I2 protein.

  • Tested applications

    Suitable for: ELISA, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human COX4I2 aa 31-80 (internal sequence).
    Database link: Q96KJ9

  • Positive control

    • COS7 cells and K562 cell extracts treated with insulin (0.01U/ml, 15mins).
  • General notes

     This product was previously labelled as COXIV Isoform 2

     

Properties

Applications

Our Abpromise guarantee covers the use of ab70112 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/10000.
WB 1/500 - 1/1000. Detects a band of approximately 20 kDa (predicted molecular weight: 20 kDa).
ICC/IF 1/500 - 1/1000.

Target

  • Function

    This protein is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport.
  • Tissue specificity

    Highly expressed in lung.
  • Involvement in disease

    Defects in COX4I2 are the cause of exocrine pancreatic insufficiency dyserythropoietic anemia and calvarial hyperostosis (EPIDACH) [MIM:612714]. Patients present with pancreatic insufficiency, intestinal malabsorption, failure to thrive, and anemia soon after birth.
  • Sequence similarities

    Belongs to the cytochrome c oxidase IV family.
  • Cellular localization

    Mitochondrion inner membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • COX IV-2 antibody
    • COX4 2 antibody
    • COX4 antibody
    • COX42_HUMAN antibody
    • COX4B antibody
    • Cox4i2 antibody
    • COX4L2 antibody
    • COXIV 2 antibody
    • Cytochrome c oxidase subunit 4 isoform 2 antibody
    • Cytochrome c oxidase subunit 4 isoform 2 mitochondrial [Precursor] antibody
    • cytochrome c oxidase subunit IV isoform 2 (lung) antibody
    • Cytochrome c oxidase subunit IV isoform 2 antibody
    • dJ857M17.2 antibody
    • mitochondrial antibody
    • RP23 106A3.3 antibody
    see all

Images

  • ab70112 at 1/500 dilution staining COX4I2 in COS7 cells by Immunofluorescence, in the absence or presence of the immunising peptide.

  • All lanes : Anti-COX4I2 antibody (ab70112) at 1/500 dilution

    Lane 1 : K562 cell extracts treated
    with insulin (0.01U/ml, 15mins)
    Lane 2 : K562 cell extracts treated
    with insulin (0.01U/ml, 15mins) with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 20 kDa
    Observed band size: 20 kDa

References

This product has been referenced in:

  • Lobo-Jarne T  et al. Human COX7A2L Regulates Complex III Biogenesis and Promotes Supercomplex Organization Remodeling without Affecting Mitochondrial Bioenergetics. Cell Rep 25:1786-1799.e4 (2018). Read more (PubMed: 30428348) »
  • Zhang X  et al. Oxidative stress regulates cellular bioenergetics in esophageal squamous cell carcinoma cell. Biosci Rep 37:N/A (2017). Read more (PubMed: 29026004) »
See all 5 Publications for this product

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