Key features and details
- Rabbit polyclonal to COX4NB
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-COX4NB antibody
DescriptionRabbit polyclonal to COX4NB
Specificityab197658 detects endogenous level of total COX4NB protein.
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Fusion protein corresponding to Human COX4NB aa 1-210. NCBI Accession No. BC001472. The protein fusion partner is GST.
Database link: O43402
- A549, HeLa and HT29 cell lysates. Human liver cancer tissue.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.4
Preservative: 0.05% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab197658 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/30 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/500 - 1/2000. Predicted molecular weight: 24 kDa.|
Tissue specificityExpressed in liver, pancreas, heart, lung, kidney, brain, skeletal muscle, and placenta. Expression levels are highest in pancreas and moderate in heart, skeletal muscle, and placenta.
Sequence similaritiesBelongs to the UPF0172 (NOC4) family.
- Information by UniProt
- C16orf2 antibody
- C16orf4 antibody
- COX4 neighbor antibody
All lanes : Anti-COX4NB antibody (ab197658) at 1/600 dilution
Lane 1 : A549 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : HT29 cell lysate
Lysates/proteins at 40 µg per lane.
Predicted band size: 24 kDa
Exposure time: 1 minute
Immunohistochemical analysis of paraffin-embedded liver cancer tissue labeling COX4NB using ab197658 at a 1/30 dilution.
ab197658 has not yet been referenced specifically in any publications.