Anti-COX4NB antibody (ab197658)
Key features and details
- Rabbit polyclonal to COX4NB
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-COX4NB antibody -
Description
Rabbit polyclonal to COX4NB -
Host species
Rabbit -
Specificity
ab197658 detects endogenous level of total COX4NB protein. -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Fusion protein corresponding to Human COX4NB aa 1-210. NCBI Accession No. BC001472. The protein fusion partner is GST.
Database link: O43402 -
Positive control
- A549, HeLa and HT29 cell lysates. Human liver cancer tissue.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.4
Preservative: 0.05% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab197658 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/30 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| WB | 1/500 - 1/2000. Predicted molecular weight: 24 kDa. |
Target
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Tissue specificity
Expressed in liver, pancreas, heart, lung, kidney, brain, skeletal muscle, and placenta. Expression levels are highest in pancreas and moderate in heart, skeletal muscle, and placenta. -
Sequence similarities
Belongs to the UPF0172 (NOC4) family. - Information by UniProt
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Database links
- Entrez Gene: 10328 Human
- Entrez Gene: 18117 Mouse
- Entrez Gene: 361425 Rat
- Omim: 604886 Human
- SwissProt: O43402 Human
- SwissProt: O70378 Mouse
- SwissProt: Q5FVL2 Rat
- Unigene: 173162 Human
see all -
Alternative names
- C16orf2 antibody
- C16orf4 antibody
- COX4 neighbor antibody
see all
Images
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Western blot - Anti-COX4NB antibody (ab197658)All lanes : Anti-COX4NB antibody (ab197658) at 1/600 dilution
Lane 1 : A549 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : HT29 cell lysate
Lysates/proteins at 40 µg per lane.
Predicted band size: 24 kDa
Exposure time: 1 minute -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX4NB antibody (ab197658)Immunohistochemical analysis of paraffin-embedded liver cancer tissue labeling COX4NB using ab197658 at a 1/30 dilution.
Protocols
Datasheets and documents
References (0)
ab197658 has not yet been referenced specifically in any publications.
