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Dear Technical Support/Custom Service,
We purchased ab110262 on March 26, 2012 (Order No. 1059903), and unfortunately it did not perform in our experiments. Please see attached image. Would it be possible to receive a substitution/replacement (MS408 COXIV lot B0659)?
Asked on May 22 2012
Thank you for your enquiry. I am sorry to hear this vial of ab110262 is giving you trouble.
And thanks for including the western blot images. If I understand you correctly, you first probed the membrane with anti-CORE2 antibody and then probed the same membrane with COXVA antibody. It appears that the essentially the same signal is showing through on the COXVA staining as was present on the CORE2 staining.
I think the first blot was not completely stripped of signal. So the second blot is difficult to interpret.
I would recommend running a new gel and blotting for COXVA.
I hope this is helpful and I look forward to hearing if these suggestions prove useful.
Answered on May 22 2012