Key features and details
- Rabbit polyclonal to COX5A
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-COX5A antibody
See all COX5A primary antibodies
DescriptionRabbit polyclonal to COX5A
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow, Chimpanzee, Baboon, Macaque monkey, Gorilla, Orangutan
Synthetic peptide within Human COX5A aa 50 to the C-terminus conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
Database link: P20674
- This antibody gave a positive signal in Human Heart Mitochondrial lysate. It also gave a positive signal in IHC-P in FFPE human heart tissue sections.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab156445 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 13 kDa (predicted molecular weight: 17 kDa).|
FunctionThis is the heme A-containing chain of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport.
Sequence similaritiesBelongs to the cytochrome c oxidase subunit 5A family.
Cellular localizationMitochondrion inner membrane.
- Information by UniProt
- COX antibody
- COX VA antibody
- COX5A antibody
IHC image of COX5A staining in human heart formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab156445, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Anti-COX5A antibody (ab156445) at 1 µg/ml + Human Heart Mitochondrial Lysate at 25 µg
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 17 kDa
Observed band size: 13 kDa why is the actual band size different from the predicted?
Additional bands at: 30 kDa (possible non-specific binding)
Exposure time: 8 minutes
The band observed at 13 kDa could potentially be a cleaved form of COX5A due to the presence of a 41 amino acid transit peptide.
This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab156445 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406
ab156445 has been referenced in 1 publication.
- Huang Y et al. The Dual Role of AQP4 in Cytotoxic and Vasogenic Edema Following Spinal Cord Contusion and Its Possible Association With Energy Metabolism via COX5A. Front Neurosci 13:584 (2019). PubMed: 31258460