Overview

  • Product name

    Anti-COX5A antibody [EPR14207(B)]
    See all COX5A primary antibodies
  • Description

    Rabbit monoclonal [EPR14207(B)] to COX5A
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, Flow Cyt, ICC/IF, IPmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human COX5A aa 1-100. The exact sequence is proprietary.
    Database link: P20674

  • Positive control

    • HepG2, HeLa and A431 cell lysates; Human brain and kidney tissues; Hela cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR14207(B)
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab181226 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/250.
WB 1/1000 - 1/10000. Detects a band of approximately 13 kDa (predicted molecular weight: 17 kDa).
Flow Cyt 1/10.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

ICC/IF 1/100 - 1/250.
IP 1/40 - 1/60.

Target

  • Function

    This is the heme A-containing chain of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport.
  • Sequence similarities

    Belongs to the cytochrome c oxidase subunit 5A family.
  • Cellular localization

    Mitochondrion inner membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • COX antibody
    • COX VA antibody
    • COX5A antibody
    • COX5A_HUMAN antibody
    • Cytochrome c oxidase polypeptide Va antibody
    • Cytochrome c oxidase polypeptide, mitochondrial antibody
    • Cytochrome c oxidase subunit 5A antibody
    • Cytochrome c oxidase subunit 5A, mitochondrial antibody
    • Cytochrome c oxidase subunit Va antibody
    • mitochondrial antibody
    • Mitochondrial cytochrome c oxidase subunit Va antibody
    • VA antibody
    see all

Images

  • All lanes : Anti-COX5A antibody [EPR14207(B)] (ab181226) at 1/10000 dilution

    Lane 1 : HepG2 cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : A431 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 17 kDa
    Observed band size: 13 kDa
    why is the actual band size different from the predicted?

  • Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling COX5A with ab181226 at 1/250 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling COX5A with ab181226 at 1/100 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

  • Immunofluorescent analysis of acetone-fixed HeLa cells labeling COX5A with ab181226 at 1/250 dilution, followed by Goat anti rabbit IgG (Alexa Fluor® 555) secondary antibody at 1/200 dilution (red). Counter stained with Dapi (blue).

  • Flow cytometric analysis of 2% paraformaldehyde-fixed HeLa cells labeling COX5A with ab181226 at 1/10 dilution (red) compared to a Rabbit monoclonal IgG isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.

  • Western blot analysis of HeLa cell lysate immunoprecipitated with ab181226 at 1/50 dilution.

    Secondary: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution.

References

ab181226 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab181226.
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