Product nameAnti-COX5B antibody [EPR14439(B)]
See all COX5B primary antibodies
DescriptionRabbit monoclonal [EPR14439(B)] to COX5B
Tested applicationsSuitable for: Flow Cyt, IHC-P, WB, ICC/IF, IPmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human COX5B aa 1-100. The exact sequence is proprietary.
Database link: P10606
- Human fetal heart lysate; HeLa and HepG2 cells; Human kidney tissue; U87-MG, HepG2 and HeLa cell lysates.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
PurityTissue culture supernatant
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Integration of energy
Our Abpromise guarantee covers the use of ab180136 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|IHC-P||1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/10000 - 1/50000. Predicted molecular weight: 14 kDa.|
FunctionThis protein is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport.
Sequence similaritiesBelongs to the cytochrome c oxidase subunit 5B family.
Cellular localizationMitochondrion inner membrane.
- Information by UniProt
- COX 5B antibody
- COX5B antibody
- COX5B_HUMAN antibody
All lanes : Anti-COX5B antibody [EPR14439(B)] (ab180136) at 1/50000 dilution
Lane 1 : Human fetal heart lysate
Lane 2 : HepG2 cell lysate
Lane 3 : HeLa cell lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 14 kDa
Immunofluorescence analysis of HepG2 cells labeling COX5B using ab180136 at 1/1000 dilution. A Goat anti rabbit IgG (Dylight 555) at 1/250 dilution was used as secondary antibody. Cells were fixed with 4% paraformaldehyde. Counterstain: DAPI.
Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling COX5B using ab180136 at 1/500 dilution. A Goat anti rabbit IgG (HRP) prediluted was used as secondary. Counterstain: Hematoxylin.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunoprecipitation analysis of Human fetal heart lysate labeling COX5B using ab180136 at 1/70 dilution. A Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 was used as secondary antibody.
Anti-COX5B antibody [EPR14439(B)] (ab180136) at 1/10000 dilution + U87-MG cell lysate at 20 µg
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 14 kDa
Flow cytometry analysis of HeLa cells labeling COX5B using ab180136 at 1/10 dilution. A Goat anti rabbit IgG (FITC) at 1/75 dilution was used as secondary antibody. Cells were fixed with 2% paraformaldehyde. Isotype control: Rabbit IgG.
This product has been referenced in:
- Stein J et al. Systematic expression analysis of the mitochondrial respiratory chain protein subunits identifies COX5B as a prognostic marker in clear cell renal cell carcinoma. Int J Urol 26:910-916 (2019). Read more (PubMed: 31280487) »
- Mot AI et al. Circumventing the Crabtree Effect: A method to induce lactate consumption and increase oxidative phosphorylation in cell culture. Int J Biochem Cell Biol 79:128-138 (2016). Read more (PubMed: 27590850) »