Key features and details
- Mouse monoclonal [14A3AD2BH4] to COX6A1
- Suitable for: Flow Cyt, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG1
Product nameAnti-COX6A1 antibody [14A3AD2BH4]
See all COX6A1 primary antibodies
DescriptionMouse monoclonal [14A3AD2BH4] to COX6A1
Specificityab110265 recognises the Liver isoform in human, rat, mouse.
Tested applicationsSuitable for: Flow Cyt, WBmore details
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Rabbit, Cow, Dog
Synthetic peptide. This information is considered to be commercially sensitive.
- HepG2, Human, Mouse and Rat heart lysate.
Storage instructionsShipped at 4°C. Store at +4°C. Do Not Freeze.
Storage bufferPreservative: 0.02% Sodium azide
Constituent: HEPES buffered saline
Concentration information loading...
Purification notesab110265 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.
Light chain typekappa
Our Abpromise guarantee covers the use of ab110265 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 1µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|WB||Use a concentration of 5 µg/ml. Predicted molecular weight: 12 kDa.|
FunctionThis protein is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport.
Sequence similaritiesBelongs to the cytochrome c oxidase subunit 6A family.
Cellular localizationMitochondrion inner membrane.
- Information by UniProt
- COX VIa L antibody
- COX VIa-L antibody
- COX6A antibody
Overlay histogram showing HepG2 cells stained with ab110265 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab110265, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HepG2 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
All lanes : Anti-COX6A1 antibody [14A3AD2BH4] (ab110265) at 5 µg/ml
Lane 1 : Human Heart Lysate
Lane 2 : Rat Heart Lysate
Lane 3 : Mouse Heart Lysate
Lane 4 : HepG2 cell lysate
Predicted band size: 12 kDa
Extra bands in the mouse sample (lane 3) are due to the reaction of the IgG-specific goat anti-mouse secondary antibody with residual mouse blood in the heart tissue, as it is very difficult to entirely remove the blood from these small organs.
ab110265 has been referenced in 2 publications.
- Tamiya G et al. A mutation of COX6A1 causes a recessive axonal or mixed form of Charcot-Marie-Tooth disease. Am J Hum Genet 95:294-300 (2014). PubMed: 25152455
- Sohal RS et al. Age-related decrease in expression of mitochondrial DNA encoded subunits of cytochrome c oxidase in Drosophila melanogaster. Mech Ageing Dev 129:558-61 (2008). PubMed: 18538373