Key features and details
- Mouse monoclonal [3F9D3D11AF6] to COX6B1
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Rat, Cow, Human
- Isotype: IgG1
Product nameAnti-COX6B1 antibody [3F9D3D11AF6]
See all COX6B1 primary antibodies
DescriptionMouse monoclonal [3F9D3D11AF6] to COX6B1
Tested applicationsSuitable for: ICC/IF, WBmore details
Species reactivityReacts with: Mouse, Rat, Cow, Human
Full length native protein (purified). This information is considered to be commercially sensitive.
- Human, cow, rat and mouse heart. IF/ICC: HeLa cell line.
This antibody clone is manufactured by Abcam.
Product was previously marketed under the MitoSciences sub-brand.
This product was previously labelled as Cytochrome C Oxidase subunit VIb
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C. Do Not Freeze.
Storage bufferPreservative: 0.02% Sodium azide
Constituent: HEPES buffered saline
Concentration information loading...
Purification notesNear homogeneity as judged by SDS-PAGE. ab110266 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.
Light chain typekappa
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Integration of energy
Our Abpromise guarantee covers the use of ab110266 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 5 µg/ml.|
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 10 kDa.|
FunctionConnects the two COX monomers into the physiological dimeric form.
Involvement in diseaseDefects in COX6B1 are a cause of mitochondrial complex IV deficiency (MT-C4D) [MIM:220110]; also known as cytochrome c oxidase deficiency. A disorder of the mitochondrial respiratory chain with heterogeneous clinical manifestations, ranging from isolated myopathy to severe multisystem disease affecting several tissues and organs. Features include hypertrophic cardiomyopathy, hepatomegaly and liver dysfunction, hypotonia, muscle weakness, excercise intolerance, developmental delay, delayed motor development and mental retardation. A subset of patients manifest Leigh syndrome.
Sequence similaritiesBelongs to the cytochrome c oxidase subunit 6B family.
Cellular localizationMitochondrion intermembrane space.
- Information by UniProt
- COX 6B antibody
- COX VIb 1 antibody
- COX VIb-1 antibody
ICC/IF image of ab110266 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab110266, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
All lanes : Anti-COX6B1 antibody [3F9D3D11AF6] (ab110266) at 1 µg/ml
Lane 1 : Isolated mitochondria from Human Heart at 5 µg
Lane 2 : Isolated mitochondria from Bovine Heart at 4 µg
Lane 3 : Isolated mitochondria from Rat Heart at 10 µg
Lane 4 : Isolated mitochondria from Mouse Heart at 10 µg
Predicted band size: 10 kDa
ab110266 has been referenced in 11 publications.
- Protasoni M et al. Respiratory supercomplexes act as a platform for complex III-mediated maturation of human mitochondrial complexes I and IV. EMBO J 39:e102817 (2020). PubMed: 31912925
- Signes A et al. APOPT1/COA8 assists COX assembly and is oppositely regulated by UPS and ROS. EMBO Mol Med 11:N/A (2019). PubMed: 30552096
- Mohanraj K et al. Inhibition of proteasome rescues a pathogenic variant of respiratory chain assembly factor COA7. EMBO Mol Med 11:N/A (2019). PubMed: 30885959
- Kim SE et al. Upregulation of cytochrome c oxidase subunit 6b1 (Cox6b1) and formation of mitochondrial supercomplexes: implication of Cox6b1 in the effect of calorie restriction. Age (Dordr) 37:9787 (2015). PubMed: 25929654
- Bourens M et al. Human COX20 cooperates with SCO1 and SCO2 to mature COX2 and promote the assembly of cytochrome c oxidase. Hum Mol Genet N/A:N/A (2014). Human . PubMed: 24403053