Overview

  • Product name

    Anti-COX6B1 antibody [3F9D3D11AF6]
    See all COX6B1 primary antibodies
  • Description

    Mouse monoclonal [3F9D3D11AF6] to COX6B1
  • Host species

    Mouse
  • Tested applications

    Suitable for: ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Cow, Human
  • Immunogen

    Full length native protein (purified). This information is considered to be commercially sensitive.

  • Positive control

    • Human, cow, rat and mouse heart. IF/ICC: HeLa cell line.
  • General notes

    This antibody clone is manufactured by Abcam.

    Product was previously marketed under the MitoSciences sub-brand.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

     This product was previously labelled as Cytochrome C Oxidase subunit VIb

     

Properties

Applications

Our Abpromise guarantee covers the use of ab110266 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 10 kDa.

Target

  • Function

    Connects the two COX monomers into the physiological dimeric form.
  • Involvement in disease

    Defects in COX6B1 are a cause of mitochondrial complex IV deficiency (MT-C4D) [MIM:220110]; also known as cytochrome c oxidase deficiency. A disorder of the mitochondrial respiratory chain with heterogeneous clinical manifestations, ranging from isolated myopathy to severe multisystem disease affecting several tissues and organs. Features include hypertrophic cardiomyopathy, hepatomegaly and liver dysfunction, hypotonia, muscle weakness, excercise intolerance, developmental delay, delayed motor development and mental retardation. A subset of patients manifest Leigh syndrome.
  • Sequence similarities

    Belongs to the cytochrome c oxidase subunit 6B family.
  • Cellular localization

    Mitochondrion intermembrane space.
  • Information by UniProt
  • Database links

  • Alternative names

    • COX 6B antibody
    • COX VIb 1 antibody
    • COX VIb-1 antibody
    • COX6B antibody
    • COX6B1 antibody
    • COXG antibody
    • COXVIb1 antibody
    • CX6B1_HUMAN antibody
    • Cytochrome c oxidase subunit 6B1 antibody
    • Cytochrome c oxidase subunit VIb antibody
    • Cytochrome c oxidase subunit VIb isoform 1 antibody
    • cytochrome c oxidase subunit VIb polypeptide 1 (ubiquitous) antibody
    • Cytochrome c oxidase subunit Vib polypeptide 1 antibody
    see all

Images

  • ICC/IF image of ab110266 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab110266, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • All lanes : Anti-COX6B1 antibody [3F9D3D11AF6] (ab110266) at 1 µg/ml

    Lane 1 : Isolated mitochondria from Human Heart at 5 µg
    Lane 2 : Isolated mitochondria from Bovine Heart at 4 µg
    Lane 3 : Isolated mitochondria from Rat Heart at 10 µg
    Lane 4 : Isolated mitochondria from Mouse Heart at 10 µg

    Predicted band size: 10 kDa

References

This product has been referenced in:

  • Signes A  et al. APOPT1/COA8 assists COX assembly and is oppositely regulated by UPS and ROS. EMBO Mol Med 11:N/A (2019). Read more (PubMed: 30552096) »
  • Mohanraj K  et al. Inhibition of proteasome rescues a pathogenic variant of respiratory chain assembly factor COA7. EMBO Mol Med 11:N/A (2019). Read more (PubMed: 30885959) »
See all 10 Publications for this product

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