Overview

  • Product name

    Anti-COX6B1 antibody [EPR7647]
    See all COX6B1 primary antibodies
  • Description

    Rabbit monoclonal [EPR7647] to COX6B1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt
  • Species reactivity

    Reacts with: Human
  • Immunogen

    corresponding to Human COX6B1 aa 50-150 (C terminal).
    Database link: P14854

  • Positive control

    • HL60, HeLa, HepG2 and Caco 2 cell lysates; HepG2 cells;. Human colon tissue.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

     This product was previously labelled as Cytochrome C Oxidase subunit VIb

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab131277 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 10 kDa (predicted molecular weight: 10 kDa).
IP 1/10 - 1/100.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/50 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt.
  • Target

    • Function

      Connects the two COX monomers into the physiological dimeric form.
    • Involvement in disease

      Defects in COX6B1 are a cause of mitochondrial complex IV deficiency (MT-C4D) [MIM:220110]; also known as cytochrome c oxidase deficiency. A disorder of the mitochondrial respiratory chain with heterogeneous clinical manifestations, ranging from isolated myopathy to severe multisystem disease affecting several tissues and organs. Features include hypertrophic cardiomyopathy, hepatomegaly and liver dysfunction, hypotonia, muscle weakness, excercise intolerance, developmental delay, delayed motor development and mental retardation. A subset of patients manifest Leigh syndrome.
    • Sequence similarities

      Belongs to the cytochrome c oxidase subunit 6B family.
    • Cellular localization

      Mitochondrion intermembrane space.
    • Information by UniProt
    • Database links

    • Alternative names

      • COX 6B antibody
      • COX VIb 1 antibody
      • COX VIb-1 antibody
      • COX6B antibody
      • COX6B1 antibody
      • COXG antibody
      • COXVIb1 antibody
      • CX6B1_HUMAN antibody
      • Cytochrome c oxidase subunit 6B1 antibody
      • Cytochrome c oxidase subunit VIb antibody
      • Cytochrome c oxidase subunit VIb isoform 1 antibody
      • cytochrome c oxidase subunit VIb polypeptide 1 (ubiquitous) antibody
      • Cytochrome c oxidase subunit Vib polypeptide 1 antibody
      see all

    Images

    • Immunohistochemical analysis of paraffin-embedded Human colon tissue labelling COX6B1 with ab131277 at 1/50 dilution.

    • All lanes : Anti-COX6B1 antibody [EPR7647] (ab131277) at 1/1000 dilution

      Lane 1 : HL60 cell lysate
      Lane 2 : HepG2 cell lysate
      Lane 3 : HeLa cell lysate
      Lane 4 : Caco 2 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 10 kDa

    • Immunofluorescent analysis of HepG2 cells labelling COX6B1 with ab131277 at 1/50 dilution.

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    ab131277 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Stomach)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: TRIS-EDTA Buffer pH 9,0
    Permeabilization
    Yes - Wash Buffer from Dako with Tween
    Specification
    Stomach
    Fixative
    Paraformaldehyde

    Mr. Rudolf Jung

    Verified customer

    Submitted Oct 01 2015

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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