Overview

  • Product name

  • Description

    Rabbit polyclonal to COX7A1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Chimpanzee
  • Immunogen

    Synthetic peptide corresponding to Human COX7A1 aa 1 to the C-terminus conjugated to keyhole limpet haemocyanin.
    Database link: P24310

  • Positive control

    • This antibody gave a positive signal in the following Human tissue lysates: Adult Heart; Fetal Heart; Mitochondrial Extract from Human Heart. This antibody gave a positive result in IHC in the following FFPE tissue: Human heart muscle.

Properties

Applications

Our Abpromise guarantee covers the use of ab134989 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 9 kDa (predicted molecular weight: 9 kDa).
IHC-P Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    This protein is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport.
  • Sequence similarities

    Belongs to the cytochrome c oxidase VIIa family.
  • Cellular localization

    Mitochondrion inner membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • COX7A antibody
    • COX7A1 antibody
    • COX7AH antibody
    • COX7AM antibody
    • CX7A1_HUMAN antibody
    • Cytochrome c oxidase subunit 7A1 antibody
    • Cytochrome c oxidase subunit 7A1 mitochondrial antibody
    • Cytochrome c oxidase subunit VIIa H antibody
    • Cytochrome c oxidase subunit VIIa heart antibody
    • Cytochrome c oxidase subunit VIIa heart/muscle isoform antibody
    • Cytochrome c oxidase subunit VIIa M antibody
    • Cytochrome c oxidase subunit VIIa muscle antibody
    • Cytochrome c oxidase subunit VIIa polypeptide 1 (muscle) antibody
    • Cytochrome c oxidase subunit VIIa polypeptide 1 antibody
    • Cytochrome c oxidase subunit VIIa-H antibody
    • Cytochrome c oxidase subunit VIIa-heart antibody
    • Cytochrome c oxidase subunit VIIa-M antibody
    • Cytochrome c oxidase subunit VIIa-muscle antibody
    • mitochondrial antibody
    see all

Images

  • IHC image of COX7A1 staining in Human Heart muscle formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab134989, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

     

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • All lanes : Anti-COX7A1 antibody (ab134989) at 1 µg/ml

    Lane 1 : Heart (Human) Tissue Lysate - adult normal tissue
    Lane 2 : Human Heart Mitochondrial Lysate
    Lane 3 : Heart (Human) Whole Cell Lysate - fetal normal tissue

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 9 kDa
    Observed band size: 9 kDa
    Additional bands at: 38 kDa (possible non-specific binding), 55 kDa (possible non-specific binding)


    Exposure time: 8 minutes


    This blot was produced using a 10% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab134989 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

References

This product has been referenced in:

  • Lobo-Jarne T  et al. Human COX7A2L Regulates Complex III Biogenesis and Promotes Supercomplex Organization Remodeling without Affecting Mitochondrial Bioenergetics. Cell Rep 25:1786-1799.e4 (2018). Read more (PubMed: 30428348) »
See 1 Publication for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab134989.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up