Key features and details
- Rabbit polyclonal to COX7A1
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-COX7A1 antibody
DescriptionRabbit polyclonal to COX7A1
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee
Synthetic peptide corresponding to Human COX7A1 aa 1 to the C-terminus conjugated to keyhole limpet haemocyanin.
Database link: P24310
- This antibody gave a positive signal in the following Human tissue lysates: Adult Heart; Fetal Heart; Mitochondrial Extract from Human Heart. This antibody gave a positive result in IHC in the following FFPE tissue: Human heart muscle.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab134989 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 9 kDa (predicted molecular weight: 9 kDa).|
|IHC-P||Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionThis protein is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport.
Sequence similaritiesBelongs to the cytochrome c oxidase VIIa family.
Cellular localizationMitochondrion inner membrane.
- Information by UniProt
- COX7A antibody
- COX7A1 antibody
- COX7AH antibody
IHC image of COX7A1 staining in Human Heart muscle formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab134989, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
All lanes : Anti-COX7A1 antibody (ab134989) at 1 µg/ml
Lane 1 : Heart (Human) Tissue Lysate - adult normal tissue
Lane 2 : Human Heart Mitochondrial Lysate
Lane 3 : Heart (Human) Whole Cell Lysate - fetal normal tissue
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 9 kDa
Observed band size: 9 kDa
Additional bands at: 38 kDa (possible non-specific binding), 55 kDa (possible non-specific binding)
Exposure time: 8 minutes
This blot was produced using a 10% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab134989 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
ab134989 has been referenced in 1 publication.
- Lobo-Jarne T et al. Human COX7A2L Regulates Complex III Biogenesis and Promotes Supercomplex Organization Remodeling without Affecting Mitochondrial Bioenergetics. Cell Rep 25:1786-1799.e4 (2018). PubMed: 30428348