Anti-Coxsackie Adenovirus Receptor/hCAR antibody (ab100811)
Key features and details
- Rabbit polyclonal to Coxsackie Adenovirus Receptor/hCAR
- Suitable for: WB, IP, IHC-P
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-Coxsackie Adenovirus Receptor/hCAR antibody
See all Coxsackie Adenovirus Receptor/hCAR primary antibodies -
Description
Rabbit polyclonal to Coxsackie Adenovirus Receptor/hCAR -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Platypus -
Immunogen
Synthetic peptide corresponding to Human Coxsackie Adenovirus Receptor/hCAR aa 300-400. NP_001329.1
Database link: P78310 -
Positive control
- HeLa whole cell lysate (ab150035)
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab100811 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (2) |
1/2000 - 1/10000. Predicted molecular weight: 40 kDa.
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IP | (1) |
Use at 2-5 µg/mg of lysate.
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IHC-P |
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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WB
1/2000 - 1/10000. Predicted molecular weight: 40 kDa. |
IP
Use at 2-5 µg/mg of lysate. |
IHC-P
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Component of the epithelial apical junction complex that is essential for the tight junction integrity. Proposed to function as a homophilic cell adhesion molecule. Recruits MPDZ to intercellular contact sites. Probably involved in transepithelial migration of polymorphonuclear leukocytes (PMN) through adhesive interactions with AMICA1/JAML located in the plasma membrane of PMN. -
Tissue specificity
Expressed in pancreas, brain, heart, small intestine, testis, prostate and at a lower level in liver and lung. Isoform 5 is ubiquitously expressed. Isoform 3 is expressed in heart, lung and pancreas. In skeletal muscle, isoform 1 is found at the neuromuscular junction and isoform 2 is found in blood vessels. In cardiac muscle, isoform 1 and isoform 2 are found at intercalated disks. In heart expressed in subendothelial layers of the vessel wall but not in the luminal endothelial surface. Expression is elevated in hearts with dilated cardiomyopathy. -
Sequence similarities
Contains 2 Ig-like C2-type (immunoglobulin-like) domains. -
Domain
The Ig-like C2-type 1 domain probably mediates homodimerization and interaction with JAML.
The PDZ-binding motif mediates interaction with MPDZ and BAIAP1. -
Post-translational
modificationsN-glycosylated.
Palmitoylated on Cys-259 and/or Cys-260; required for proper localization to the plasma membrane. -
Cellular localization
Secreted and Cell membrane. Cell junction > tight junction. Cell junction > adherens junction. Basolateral cell membrane. In epithelial cells localizes to the apical junction complex composced of tight and adherens junctions. In airway epithelial cells localized to basolateral membrane but not to apical surface. - Information by UniProt
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Database links
- Entrez Gene: 281733 Cow
- Entrez Gene: 403772 Dog
- Entrez Gene: 1525 Human
- Entrez Gene: 13052 Mouse
- Entrez Gene: 100174624 Orangutan
- Entrez Gene: 397333 Pig
- Entrez Gene: 100302411 Rabbit
- Entrez Gene: 89843 Rat
see all -
Alternative names
- 46 kD coxsackievirus and adenovirus receptor CAR protein antibody
- 46 kD coxsackievirus and adenovirus receptor protein antibody
- CAR antibody
see all
Images
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All lanes : Anti-Coxsackie Adenovirus Receptor/hCAR antibody (ab100811) at 0.1 µg/ml
Lane 1 : HeLa Whole Cell Lysate at 50 µg
Lane 2 : HeLa Whole Cell Lysate at 15 µg
Lane 3 : HeLa Whole Cell Lysate at 5 µg
Predicted band size: 40 kDa
Exposure time: 3 minutes -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling Coxsackie Adenovirus Receptor/hCAR with ab100811 at 1/1000 (1µg/ml). Detection: Peroxidase Substrate.
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ab100811 at 0.4 µg/ml staining Coxsackie Adenovirus Receptor/hCAR in HeLa cell lysate immunoprecipitated using ab100811 at 3 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Exposure time: 30 seconds
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab100811 has been referenced in 4 publications.
- Huang Y et al. A SIRPa-Fc fusion protein enhances the antitumor effect of oncolytic adenovirus against ovarian cancer. Mol Oncol 14:657-668 (2020). PubMed: 31899582
- Weider T et al. Upregulation of HLA Class I and Antiviral Tissue Responses in Hashimoto's Thyroiditis. Thyroid 30:432-442 (2020). PubMed: 31910110
- Lalwani A et al. ß Cell Hypoxia-Inducible Factor-1a Is Required for the Prevention of Type 1 Diabetes. Cell Rep 27:2370-2384.e6 (2019). PubMed: 31116982
- Ulasov IV et al. Tamoxifen improves cytopathic effect of oncolytic adenovirus in primary glioblastoma cells mediated through autophagy. Oncotarget 6:3977-87 (2015). WB . PubMed: 25738357