Overview

  • Product name

    Anti-Coxsackie Adenovirus Receptor/hCAR antibody
    See all Coxsackie Adenovirus Receptor/hCAR primary antibodies
  • Description

    Rabbit polyclonal to Coxsackie Adenovirus Receptor/hCAR
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Platypus
  • Immunogen

    Synthetic peptide corresponding to Human Coxsackie Adenovirus Receptor/hCAR aa 335-365.
    Database link: NP_001329.1

  • Positive control

  • General notes

     This product was previously labelled as Coxsackie Adenovirus Receptor

     

Properties

Applications

Our Abpromise guarantee covers the use of ab100811 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 40 kDa.
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Component of the epithelial apical junction complex that is essential for the tight junction integrity. Proposed to function as a homophilic cell adhesion molecule. Recruits MPDZ to intercellular contact sites. Probably involved in transepithelial migration of polymorphonuclear leukocytes (PMN) through adhesive interactions with AMICA1/JAML located in the plasma membrane of PMN.
  • Tissue specificity

    Expressed in pancreas, brain, heart, small intestine, testis, prostate and at a lower level in liver and lung. Isoform 5 is ubiquitously expressed. Isoform 3 is expressed in heart, lung and pancreas. In skeletal muscle, isoform 1 is found at the neuromuscular junction and isoform 2 is found in blood vessels. In cardiac muscle, isoform 1 and isoform 2 are found at intercalated disks. In heart expressed in subendothelial layers of the vessel wall but not in the luminal endothelial surface. Expression is elevated in hearts with dilated cardiomyopathy.
  • Sequence similarities

    Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
  • Domain

    The Ig-like C2-type 1 domain probably mediates homodimerization and interaction with JAML.
    The PDZ-binding motif mediates interaction with MPDZ and BAIAP1.
  • Post-translational
    modifications

    N-glycosylated.
    Palmitoylated on Cys-259 and/or Cys-260; required for proper localization to the plasma membrane.
  • Cellular localization

    Secreted and Cell membrane. Cell junction > tight junction. Cell junction > adherens junction. Basolateral cell membrane. In epithelial cells localizes to the apical junction complex composced of tight and adherens junctions. In airway epithelial cells localized to basolateral membrane but not to apical surface.
  • Information by UniProt
  • Database links

  • Alternative names

    • 46 kD coxsackievirus and adenovirus receptor CAR protein antibody
    • 46 kD coxsackievirus and adenovirus receptor protein antibody
    • CAR antibody
    • CAR4/6 antibody
    • Coxsackie virus and adenovirus receptor antibody
    • Coxsackie virus B receptor antibody
    • Coxsackievirus and adenovirus receptor antibody
    • Coxsackievirus B adenovirus receptor antibody
    • Coxsackievirus B-adenovirus receptor antibody
    • CVB3 binding protein antibody
    • CVB3-binding protein antibody
    • Cxadr antibody
    • CXADR Ig like cell adhesion molecule antibody
    • CXAR_HUMAN antibody
    • hCAR antibody
    • HCVADR antibody
    see all

Images

  • All lanes : Anti-Coxsackie Adenovirus Receptor/hCAR antibody (ab100811) at 0.1 µg/ml

    Lane 1 : HeLa Whole Cell Lysate at 50 µg
    Lane 2 : HeLa Whole Cell Lysate at 15 µg
    Lane 3 : HeLa Whole Cell Lysate at 5 µg

    Predicted band size: 40 kDa


    Exposure time: 3 minutes
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling Coxsackie Adenovirus Receptor/hCAR with ab100811 at 1/1000 (1µg/ml). Detection: Peroxidase Substrate.

  • ab100811 at 0.4 µg/ml staining Coxsackie Adenovirus Receptor/hCAR in HeLa cell lysate immunoprecipitated using ab100811 at 3 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Exposure time: 30 seconds

References

This product has been referenced in:

  • Ulasov IV  et al. Tamoxifen improves cytopathic effect of oncolytic adenovirus in primary glioblastoma cells mediated through autophagy. Oncotarget 6:3977-87 (2015). WB . Read more (PubMed: 25738357) »
See 1 Publication for this product

Customer reviews and Q&As

Application
Western blot
Sample
Mouse Tissue lysate - whole (Testis)
Gel Running Conditions
Reduced Denaturing (4-12% gradient)
Loading amount
30 µg
Specification
Testis
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Abcam user community

Verified customer

Submitted Aug 18 2014

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