Overview

  • Product name

    Anti-Coxsackie Adenovirus Receptor/hCAR antibody
    See all Coxsackie Adenovirus Receptor/hCAR primary antibodies
  • Description

    Rabbit polyclonal to Coxsackie Adenovirus Receptor/hCAR
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human Coxsackie Adenovirus Receptor/hCAR (internal sequence) conjugated to keyhole limpet haemocyanin.

  • Positive control

    • Human testis, Human liver tissues
  • General notes

     This product was previously labelled as Coxsackie Adenovirus Receptor

     

Properties

Applications

Our Abpromise guarantee covers the use of ab133380 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Component of the epithelial apical junction complex that is essential for the tight junction integrity. Proposed to function as a homophilic cell adhesion molecule. Recruits MPDZ to intercellular contact sites. Probably involved in transepithelial migration of polymorphonuclear leukocytes (PMN) through adhesive interactions with AMICA1/JAML located in the plasma membrane of PMN.
  • Tissue specificity

    Expressed in pancreas, brain, heart, small intestine, testis, prostate and at a lower level in liver and lung. Isoform 5 is ubiquitously expressed. Isoform 3 is expressed in heart, lung and pancreas. In skeletal muscle, isoform 1 is found at the neuromuscular junction and isoform 2 is found in blood vessels. In cardiac muscle, isoform 1 and isoform 2 are found at intercalated disks. In heart expressed in subendothelial layers of the vessel wall but not in the luminal endothelial surface. Expression is elevated in hearts with dilated cardiomyopathy.
  • Sequence similarities

    Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
  • Domain

    The Ig-like C2-type 1 domain probably mediates homodimerization and interaction with JAML.
    The PDZ-binding motif mediates interaction with MPDZ and BAIAP1.
  • Post-translational
    modifications

    N-glycosylated.
    Palmitoylated on Cys-259 and/or Cys-260; required for proper localization to the plasma membrane.
  • Cellular localization

    Secreted and Cell membrane. Cell junction > tight junction. Cell junction > adherens junction. Basolateral cell membrane. In epithelial cells localizes to the apical junction complex composced of tight and adherens junctions. In airway epithelial cells localized to basolateral membrane but not to apical surface.
  • Information by UniProt
  • Database links

  • Alternative names

    • 46 kD coxsackievirus and adenovirus receptor CAR protein antibody
    • 46 kD coxsackievirus and adenovirus receptor protein antibody
    • CAR antibody
    • CAR4/6 antibody
    • Coxsackie virus and adenovirus receptor antibody
    • Coxsackie virus B receptor antibody
    • Coxsackievirus and adenovirus receptor antibody
    • Coxsackievirus B adenovirus receptor antibody
    • Coxsackievirus B-adenovirus receptor antibody
    • CVB3 binding protein antibody
    • CVB3-binding protein antibody
    • Cxadr antibody
    • CXADR Ig like cell adhesion molecule antibody
    • CXAR_HUMAN antibody
    • hCAR antibody
    • HCVADR antibody
    see all

Images

  • Immunohistochemical analysis of Coxsackie Adenovirus Receptor/hCAR expression in Formalin-Fixed, Paraffin-Embedded Human testis tissue using 10 µg/ml ab133380.

  • Immunohistochemical analysis of Coxsackie Adenovirus Receptor/hCAR expression in Formalin-Fixed, Paraffin-Embedded Human liver tissue using 10 µg/ml ab133380.

References

ab133380 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Question
Answer

The immunogenic peptide is located downstream of amino acid 90 but upstream of amino acid 340. So, it would not be able to recognize isoforms 3, 4, and 5 as defined by Uniprot P78310. http://www.uniprot.org/uniprot/P78310

The sequence of the immunogen does not correspond to amino acids 238 – 258.

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Question
Answer

The immunogenic peptide used to generate this antibody is found in isoforms 1 and 2 according to Swiss-Prot accession number P78310.

We do not have cell lines that express isoforms 1 and 2, so I cannot say if the antibody can distinguish the two separate isoforms in tissue.

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