Product nameAnti-Coxsackie Adenovirus Receptor/hCAR antibody
See all Coxsackie Adenovirus Receptor/hCAR primary antibodies
DescriptionRabbit polyclonal to Coxsackie Adenovirus Receptor/hCAR
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Cow, Orangutan
Recombinant fragment corresponding to Human Coxsackie Adenovirus Receptor/hCAR aa 20-237 (extracellular).
LSITTPEEMIE KAKGETAYLP CKFTLSPEDQ GPLDIEWLIS PADNQKVDQV IILYSGDKIY DDYYPDLKGR VHFTSNDLKS GDASINVTNL QLSDIGTYQC KVKKAPGVAN KKIHLVVLVK PSGARCYVDG SEEIGSDFKI KCEPKEGSLP LQYEWQKLSD SQKMPTSWLA EMTSSVISVK NASSEYSGTY SCTVRNRVGS DQCLLRLNVV PPSNKAG
Database link: P78310
- HeLa, OVCAR3, SW480, HepG2, PC3 cell line lysates; Mouse liver tissue and Mouse heart tissue extracts.
This product was previously labelled as Coxsackie Adenovirus Receptor
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
- HeLa whole cell lysate (ab150035)
- HepG2 whole cell lysate (ab166833)
- Mouse liver tissue lysate - total protein (ab29301)
- HeLa whole cell lysate (ab29545)
- Mouse heart normal tissue lysate - total protein (ab30291)
- PC3 whole cell lysate (ab3954)
- SW480 whole cell lysate (ab3957)
- HepG2 whole cell lysate (ab7900)
Our Abpromise guarantee covers the use of ab180761 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/2000. Predicted molecular weight: 40 kDa.|
|IHC-P||1/50 - 1/200.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionComponent of the epithelial apical junction complex that is essential for the tight junction integrity. Proposed to function as a homophilic cell adhesion molecule. Recruits MPDZ to intercellular contact sites. Probably involved in transepithelial migration of polymorphonuclear leukocytes (PMN) through adhesive interactions with AMICA1/JAML located in the plasma membrane of PMN.
Tissue specificityExpressed in pancreas, brain, heart, small intestine, testis, prostate and at a lower level in liver and lung. Isoform 5 is ubiquitously expressed. Isoform 3 is expressed in heart, lung and pancreas. In skeletal muscle, isoform 1 is found at the neuromuscular junction and isoform 2 is found in blood vessels. In cardiac muscle, isoform 1 and isoform 2 are found at intercalated disks. In heart expressed in subendothelial layers of the vessel wall but not in the luminal endothelial surface. Expression is elevated in hearts with dilated cardiomyopathy.
Sequence similaritiesContains 2 Ig-like C2-type (immunoglobulin-like) domains.
DomainThe Ig-like C2-type 1 domain probably mediates homodimerization and interaction with JAML.
The PDZ-binding motif mediates interaction with MPDZ and BAIAP1.
Palmitoylated on Cys-259 and/or Cys-260; required for proper localization to the plasma membrane.
Cellular localizationSecreted and Cell membrane. Cell junction > tight junction. Cell junction > adherens junction. Basolateral cell membrane. In epithelial cells localizes to the apical junction complex composced of tight and adherens junctions. In airway epithelial cells localized to basolateral membrane but not to apical surface.
- Information by UniProt
- 46 kD coxsackievirus and adenovirus receptor CAR protein antibody
- 46 kD coxsackievirus and adenovirus receptor protein antibody
- CAR antibody
All lanes : Anti-Coxsackie Adenovirus Receptor/hCAR antibody (ab180761) at 1/500 dilution
Lane 1 : HeLa cell line lysate
Lane 2 : OVCAR3 cell line lysate
Lane 3 : SW480 cell line lysate
Lane 4 : HepG2 cell line lysate
Lane 5 : PC3 cell line lysate
Lane 6 : Mouse liver tissue lysate
Lane 7 : Mouse heart tissue lysate
Predicted band size: 40 kDa