Key features and details
- Rabbit polyclonal to Coxsackie Adenovirus Receptor/hCAR
- Suitable for: WB, Flow Cyt
- Reacts with: Mouse, Rat
- Isotype: IgG
Product nameAnti-Coxsackie Adenovirus Receptor/hCAR antibody
See all Coxsackie Adenovirus Receptor/hCAR primary antibodies
DescriptionRabbit polyclonal to Coxsackie Adenovirus Receptor/hCAR
Tested applicationsSuitable for: WB, Flow Cytmore details
Species reactivityReacts with: Mouse, Rat
Predicted to work with: Human
- Rat heart lysate; Mouse brain cells.
This product was previously labelled as Coxsackie Adenovirus Receptor
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 1% BSA, 50% Glycerol
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab214354 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/100 - 1/1000. Detects a band of approximately 38 kDa (predicted molecular weight: 40 kDa).|
|Flow Cyt||1/20 - 1/100.|
FunctionComponent of the epithelial apical junction complex that is essential for the tight junction integrity. Proposed to function as a homophilic cell adhesion molecule. Recruits MPDZ to intercellular contact sites. Probably involved in transepithelial migration of polymorphonuclear leukocytes (PMN) through adhesive interactions with AMICA1/JAML located in the plasma membrane of PMN.
Tissue specificityExpressed in pancreas, brain, heart, small intestine, testis, prostate and at a lower level in liver and lung. Isoform 5 is ubiquitously expressed. Isoform 3 is expressed in heart, lung and pancreas. In skeletal muscle, isoform 1 is found at the neuromuscular junction and isoform 2 is found in blood vessels. In cardiac muscle, isoform 1 and isoform 2 are found at intercalated disks. In heart expressed in subendothelial layers of the vessel wall but not in the luminal endothelial surface. Expression is elevated in hearts with dilated cardiomyopathy.
Sequence similaritiesContains 2 Ig-like C2-type (immunoglobulin-like) domains.
DomainThe Ig-like C2-type 1 domain probably mediates homodimerization and interaction with JAML.
The PDZ-binding motif mediates interaction with MPDZ and BAIAP1.
Palmitoylated on Cys-259 and/or Cys-260; required for proper localization to the plasma membrane.
Cellular localizationSecreted and Cell membrane. Cell junction > tight junction. Cell junction > adherens junction. Basolateral cell membrane. In epithelial cells localizes to the apical junction complex composced of tight and adherens junctions. In airway epithelial cells localized to basolateral membrane but not to apical surface.
- Information by UniProt
- 46 kD coxsackievirus and adenovirus receptor CAR protein antibody
- 46 kD coxsackievirus and adenovirus receptor protein antibody
- CAR antibody
All lanes : Anti-Coxsackie Adenovirus Receptor/hCAR antibody (ab214354) at 1/200 dilution
Lane 1 : Rat heart lysate
Lane 2 : Rat embryo lysate
All lanes : Goat Anti-Rabbit IgG Antibody (H+L), HRP at 1/3000 dilution
Predicted band size: 40 kDa
Flow cytometric analysis of mouse brain cells labeling Coxsackie Adenovirus Receptor/hCAR with ab214354 at 1/50 dilution for 40 minutes followed by incubation with Goat Anti-Rabbit IgG FITC conjugated secondary at 1/100 (green) for 40 minutes compared to control cells (blue).
ab214354 has not yet been referenced specifically in any publications.