Anti-Coxsackie Adenovirus Receptor/hCAR antibody (ab214354)
Key features and details
- Rabbit polyclonal to Coxsackie Adenovirus Receptor/hCAR
- Suitable for: WB, Flow Cyt
- Reacts with: Mouse, Rat
- Isotype: IgG
Overview
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Product name
Anti-Coxsackie Adenovirus Receptor/hCAR antibody
See all Coxsackie Adenovirus Receptor/hCAR primary antibodies -
Description
Rabbit polyclonal to Coxsackie Adenovirus Receptor/hCAR -
Host species
Rabbit -
Tested applications
Suitable for: WB, Flow Cytmore details -
Species reactivity
Reacts with: Mouse, Rat
Predicted to work with: Human
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Immunogen
Synthetic peptide within Human Coxsackie Adenovirus Receptor/hCAR aa 20-70 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
Sequence:LSITTPEEMIE KAKGETAYLP CKFTLSPEDQ GPLDIEWLIS PADNQKVDQV
Database link: P78310 -
Positive control
- Rat heart lysate; Mouse brain cells.
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General notes
This product was previously labelled as Coxsackie Adenovirus Receptor
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 1% BSA, 50% Glycerol -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab214354 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/100 - 1/1000. Detects a band of approximately 38 kDa (predicted molecular weight: 40 kDa). | |
| Flow Cyt | 1/20 - 1/100. |
Target
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Function
Component of the epithelial apical junction complex that is essential for the tight junction integrity. Proposed to function as a homophilic cell adhesion molecule. Recruits MPDZ to intercellular contact sites. Probably involved in transepithelial migration of polymorphonuclear leukocytes (PMN) through adhesive interactions with AMICA1/JAML located in the plasma membrane of PMN. -
Tissue specificity
Expressed in pancreas, brain, heart, small intestine, testis, prostate and at a lower level in liver and lung. Isoform 5 is ubiquitously expressed. Isoform 3 is expressed in heart, lung and pancreas. In skeletal muscle, isoform 1 is found at the neuromuscular junction and isoform 2 is found in blood vessels. In cardiac muscle, isoform 1 and isoform 2 are found at intercalated disks. In heart expressed in subendothelial layers of the vessel wall but not in the luminal endothelial surface. Expression is elevated in hearts with dilated cardiomyopathy. -
Sequence similarities
Contains 2 Ig-like C2-type (immunoglobulin-like) domains. -
Domain
The Ig-like C2-type 1 domain probably mediates homodimerization and interaction with JAML.
The PDZ-binding motif mediates interaction with MPDZ and BAIAP1. -
Post-translational
modificationsN-glycosylated.
Palmitoylated on Cys-259 and/or Cys-260; required for proper localization to the plasma membrane. -
Cellular localization
Secreted and Cell membrane. Cell junction > tight junction. Cell junction > adherens junction. Basolateral cell membrane. In epithelial cells localizes to the apical junction complex composced of tight and adherens junctions. In airway epithelial cells localized to basolateral membrane but not to apical surface. - Information by UniProt
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Database links
- Entrez Gene: 1525 Human
- Entrez Gene: 13052 Mouse
- Entrez Gene: 89843 Rat
- Omim: 602621 Human
- SwissProt: P78310 Human
- SwissProt: P97792 Mouse
- SwissProt: Q9R066 Rat
- Unigene: 634837 Human
see all -
Alternative names
- 46 kD coxsackievirus and adenovirus receptor CAR protein antibody
- 46 kD coxsackievirus and adenovirus receptor protein antibody
- CAR antibody
see all
Images
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Western blot - Anti-Coxsackie Adenovirus Receptor/hCAR antibody (ab214354)All lanes : Anti-Coxsackie Adenovirus Receptor/hCAR antibody (ab214354) at 1/200 dilution
Lane 1 : Rat heart lysate
Lane 2 : Rat embryo lysate
Secondary
All lanes : Goat Anti-Rabbit IgG Antibody (H+L), HRP at 1/3000 dilution
Predicted band size: 40 kDa12% Gel.
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Flow Cytometry - Anti-Coxsackie Adenovirus Receptor/hCAR antibody (ab214354)Flow cytometric analysis of mouse brain cells labeling Coxsackie Adenovirus Receptor/hCAR with ab214354 at 1/50 dilution for 40 minutes followed by incubation with Goat Anti-Rabbit IgG FITC conjugated secondary at 1/100 (green) for 40 minutes compared to control cells (blue).
Datasheets and documents
References (0)
ab214354 has not yet been referenced specifically in any publications.
