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Microbiology Interspecies Interaction Host Virus Interaction
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Anti-Coxsackie Adenovirus Receptor/hCAR antibody (ab214354)

  • Datasheet
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Western blot - Anti-Coxsackie Adenovirus Receptor/hCAR antibody (ab214354)
  • Flow Cytometry - Anti-Coxsackie Adenovirus Receptor/hCAR antibody (ab214354)

Key features and details

  • Rabbit polyclonal to Coxsackie Adenovirus Receptor/hCAR
  • Suitable for: WB, Flow Cyt
  • Reacts with: Mouse, Rat
  • Isotype: IgG

You may also be interested in

Protein
Product image
Recombinant human Coxsackie Adenovirus Receptor/hCAR protein (His tag) (ab168893)
Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-Coxsackie Adenovirus Receptor/hCAR antibody
    See all Coxsackie Adenovirus Receptor/hCAR primary antibodies
  • Description

    Rabbit polyclonal to Coxsackie Adenovirus Receptor/hCAR
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse, Rat
    Predicted to work with: Human
  • Immunogen

    Synthetic peptide within Human Coxsackie Adenovirus Receptor/hCAR aa 20-70 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
    Sequence:

    LSITTPEEMIE KAKGETAYLP CKFTLSPEDQ GPLDIEWLIS PADNQKVDQV


    Database link: P78310
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • Rat heart lysate; Mouse brain cells.
  • General notes

     This product was previously labelled as Coxsackie Adenovirus Receptor

     

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 1% BSA, 50% Glycerol
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Microbiology
    • Interspecies Interaction
    • Host Virus Interaction
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cell Adhesion
    • Tight Junctions

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant human Coxsackie Adenovirus Receptor/hCAR protein (His tag) (ab168893)
  • Related Products

    • Recombinant Human Coxsackie Adenovirus Receptor/hCAR protein (ab168070)

Applications

Our Abpromise guarantee covers the use of ab214354 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/1000. Detects a band of approximately 38 kDa (predicted molecular weight: 40 kDa).
Flow Cyt 1/20 - 1/100.

Target

  • Function

    Component of the epithelial apical junction complex that is essential for the tight junction integrity. Proposed to function as a homophilic cell adhesion molecule. Recruits MPDZ to intercellular contact sites. Probably involved in transepithelial migration of polymorphonuclear leukocytes (PMN) through adhesive interactions with AMICA1/JAML located in the plasma membrane of PMN.
  • Tissue specificity

    Expressed in pancreas, brain, heart, small intestine, testis, prostate and at a lower level in liver and lung. Isoform 5 is ubiquitously expressed. Isoform 3 is expressed in heart, lung and pancreas. In skeletal muscle, isoform 1 is found at the neuromuscular junction and isoform 2 is found in blood vessels. In cardiac muscle, isoform 1 and isoform 2 are found at intercalated disks. In heart expressed in subendothelial layers of the vessel wall but not in the luminal endothelial surface. Expression is elevated in hearts with dilated cardiomyopathy.
  • Sequence similarities

    Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
  • Domain

    The Ig-like C2-type 1 domain probably mediates homodimerization and interaction with JAML.
    The PDZ-binding motif mediates interaction with MPDZ and BAIAP1.
  • Post-translational
    modifications

    N-glycosylated.
    Palmitoylated on Cys-259 and/or Cys-260; required for proper localization to the plasma membrane.
  • Cellular localization

    Secreted and Cell membrane. Cell junction > tight junction. Cell junction > adherens junction. Basolateral cell membrane. In epithelial cells localizes to the apical junction complex composced of tight and adherens junctions. In airway epithelial cells localized to basolateral membrane but not to apical surface.
  • Target information above from: UniProt accession P78310 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 1525 Human
    • Entrez Gene: 13052 Mouse
    • Entrez Gene: 89843 Rat
    • Omim: 602621 Human
    • SwissProt: P78310 Human
    • SwissProt: P97792 Mouse
    • SwissProt: Q9R066 Rat
    • Unigene: 634837 Human
    • Unigene: 66222 Mouse
    • Unigene: 113837 Rat
    see all
  • Alternative names

    • 46 kD coxsackievirus and adenovirus receptor CAR protein antibody
    • 46 kD coxsackievirus and adenovirus receptor protein antibody
    • CAR antibody
    • CAR4/6 antibody
    • Coxsackie virus and adenovirus receptor antibody
    • Coxsackie virus B receptor antibody
    • Coxsackievirus and adenovirus receptor antibody
    • Coxsackievirus B adenovirus receptor antibody
    • Coxsackievirus B-adenovirus receptor antibody
    • CVB3 binding protein antibody
    • CVB3-binding protein antibody
    • Cxadr antibody
    • CXADR Ig like cell adhesion molecule antibody
    • CXAR_HUMAN antibody
    • hCAR antibody
    • HCVADR antibody
    see all

Images

  • Western blot - Anti-Coxsackie Adenovirus Receptor/hCAR antibody (ab214354)
    Western blot - Anti-Coxsackie Adenovirus Receptor/hCAR antibody (ab214354)
    All lanes : Anti-Coxsackie Adenovirus Receptor/hCAR antibody (ab214354) at 1/200 dilution

    Lane 1 : Rat heart lysate
    Lane 2 : Rat embryo lysate

    Secondary
    All lanes : Goat Anti-Rabbit IgG Antibody (H+L), HRP at 1/3000 dilution

    Predicted band size: 40 kDa



    12% Gel.

  • Flow Cytometry - Anti-Coxsackie Adenovirus Receptor/hCAR antibody (ab214354)
    Flow Cytometry - Anti-Coxsackie Adenovirus Receptor/hCAR antibody (ab214354)

    Flow cytometric analysis of mouse brain cells labeling Coxsackie Adenovirus Receptor/hCAR with ab214354 at 1/50 dilution for 40 minutes followed by incubation with Goat Anti-Rabbit IgG FITC conjugated secondary at 1/100 (green) for 40 minutes compared to control cells (blue).

Protocols

  • Western blot protocols
  • Flow cytometry protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab214354? Please let us know so that we can cite the reference in this datasheet.

    ab214354 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Flow Cytometry abreview for Anti-Coxsackie Adenovirus Receptor/hCAR antibody

    Poor
    Abreviews
    Abreviews
    abreview image
    Application
    Flow Cytometry
    Sample
    Human Cell (A549)
    Permeabilization
    No
    Gating Strategy
    No gate
    Specification
    A549
    Preparation
    Cell harvesting/tissue preparation method: PBS/EDTA
    Sample buffer: PBS
    Fixation
    None
    Read More

    Ms. Leonie Herrmann

    Verified customer

    Submitted Feb 19 2019

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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