Key features and details
- Rabbit polyclonal to Coxsackie Adenovirus Receptor/hCAR
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Coxsackie Adenovirus Receptor/hCAR antibody
See all Coxsackie Adenovirus Receptor/hCAR primary antibodies
DescriptionRabbit polyclonal to Coxsackie Adenovirus Receptor/hCAR
Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow, Orangutan
- IHC-P: Human stomach tissue. WB: RT4 cell lysate. ICC/IF: U-2 OS cells.
This product was previously labelled as Coxsackie Adenovirus Receptor
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab223689 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100.
|WB||Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 40 kDa.|
|IHC-P||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionComponent of the epithelial apical junction complex that is essential for the tight junction integrity. Proposed to function as a homophilic cell adhesion molecule. Recruits MPDZ to intercellular contact sites. Probably involved in transepithelial migration of polymorphonuclear leukocytes (PMN) through adhesive interactions with AMICA1/JAML located in the plasma membrane of PMN.
Tissue specificityExpressed in pancreas, brain, heart, small intestine, testis, prostate and at a lower level in liver and lung. Isoform 5 is ubiquitously expressed. Isoform 3 is expressed in heart, lung and pancreas. In skeletal muscle, isoform 1 is found at the neuromuscular junction and isoform 2 is found in blood vessels. In cardiac muscle, isoform 1 and isoform 2 are found at intercalated disks. In heart expressed in subendothelial layers of the vessel wall but not in the luminal endothelial surface. Expression is elevated in hearts with dilated cardiomyopathy.
Sequence similaritiesContains 2 Ig-like C2-type (immunoglobulin-like) domains.
DomainThe Ig-like C2-type 1 domain probably mediates homodimerization and interaction with JAML.
The PDZ-binding motif mediates interaction with MPDZ and BAIAP1.
Palmitoylated on Cys-259 and/or Cys-260; required for proper localization to the plasma membrane.
Cellular localizationSecreted and Cell membrane. Cell junction > tight junction. Cell junction > adherens junction. Basolateral cell membrane. In epithelial cells localizes to the apical junction complex composced of tight and adherens junctions. In airway epithelial cells localized to basolateral membrane but not to apical surface.
- Information by UniProt
- 46 kD coxsackievirus and adenovirus receptor CAR protein antibody
- 46 kD coxsackievirus and adenovirus receptor protein antibody
- CAR antibody
Anti-Coxsackie Adenovirus Receptor/hCAR antibody (ab223689) at 1/250 dilution + RT4 (human urinary bladder cancer cell line) cell lysate
Predicted band size: 40 kDa
Paraffin-embedded human stomach tissue stained for Coxsackie Adenovirus Receptor/hCAR using ab223689 at 1/50 dilution in immunohistochemical analysis.
PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for Coxsackie Adenovirus Receptor/hCAR (green) using ab223689 at 4 µg/ml in ICC/IF.
ab223689 has not yet been referenced specifically in any publications.