The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 110 kDa (predicted molecular weight: 113 kDa). Additional bands of unknown identity were also consistently observed at 65kDa and 37kDa. These bands were successfully blocked by incubation with the immunizing peptide.
Necessary for centrosome duplication at different stages of procentriole formation. Collaborates with CEP97, being involved in the suppression of a cilia assembly program. Required for correct spindle formation and has a role in regulating cytokinesis and genome stability via cooperation with CALM1 and CETN2.
Highly expressed in testis. Detected at intermediate levels in spleen, thymus, prostate, small intestine, colon and peripheral blood leukocytes.
Phosphorylated by CDKs. Ubiquitinated by the SCF(Cyclin F) during G2 phase, leading to its degradation by the proteasome and preventing centrosome reduplication.
Cytoplasm > cytoskeleton > centrosome > centriole. Recruited early and then associates with the growing distal tips.