Key features and details
- Rabbit polyclonal to CP110
- Suitable for: IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-CP110 antibody
See all CP110 primary antibodies
DescriptionRabbit polyclonal to CP110
Tested applicationsSuitable for: IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
- IHC-P: Human testis, placenta and kidney tissue. ICC/IF: U-251 MG cells.
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We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab243696 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100.
FunctionNecessary for centrosome duplication at different stages of procentriole formation. Collaborates with CEP97, being involved in the suppression of a cilia assembly program. Required for correct spindle formation and has a role in regulating cytokinesis and genome stability via cooperation with CALM1 and CETN2.
Tissue specificityHighly expressed in testis. Detected at intermediate levels in spleen, thymus, prostate, small intestine, colon and peripheral blood leukocytes.
modificationsPhosphorylated by CDKs.
Ubiquitinated by the SCF(Cyclin F) during G2 phase, leading to its degradation by the proteasome and preventing centrosome reduplication.
Cellular localizationCytoplasm > cytoskeleton > centrosome > centriole. Recruited early and then associates with the growing distal tips.
- Information by UniProt
- 110 kDa centrosomal protein antibody
- CCP110 antibody
- Centriolar coiled coil protein 110kDa antibody
Paraformaldehyde-fixed, Triton X-100 permeabilized U-251 MG (human brain glioma cell line) cells labeling CP110 (Green) with ab243696 at 4 µg/mL in ICC/IF analysis.
Formalin-fixed, paraffin-embedded human kidney tissue stained for CP110 using ab243696 at 1/200 dilution in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded human placenta tissue stained for CP110 using ab243696 at 1/200 dilution in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded human skeletal muscle tissue stained for CP110 using ab243696 at 1/200 dilution in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded human testis tissue stained for CP110 using ab243696 at 1/200 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab243696 has not yet been referenced specifically in any publications.