Overview

  • Product name

  • Description

    Rabbit polyclonal to CP110
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Pig, Chimpanzee, Ferret, Rhesus monkey, Gorilla
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids within residues 575-625 of Human CP110 (CAH18111.1).

  • Positive control

    • HeLa and 293T whole cell lysates

Properties

Applications

Our Abpromise guarantee covers the use of ab99337 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 113 kDa.
IP Use at 2-5 µg/mg of lysate.

Target

  • Function

    Necessary for centrosome duplication at different stages of procentriole formation. Collaborates with CEP97, being involved in the suppression of a cilia assembly program. Required for correct spindle formation and has a role in regulating cytokinesis and genome stability via cooperation with CALM1 and CETN2.
  • Tissue specificity

    Highly expressed in testis. Detected at intermediate levels in spleen, thymus, prostate, small intestine, colon and peripheral blood leukocytes.
  • Post-translational
    modifications

    Phosphorylated by CDKs.
    Ubiquitinated by the SCF(Cyclin F) during G2 phase, leading to its degradation by the proteasome and preventing centrosome reduplication.
  • Cellular localization

    Cytoplasm > cytoskeleton > centrosome > centriole. Recruited early and then associates with the growing distal tips.
  • Information by UniProt
  • Database links

  • Alternative names

    • 110 kDa centrosomal protein antibody
    • CCP110 antibody
    • Centriolar coiled coil protein 110kDa antibody
    • Centrosomal protein 110 kDa antibody
    • Centrosomal protein CP110 antibody
    • Centrosomal protein of 110 kDa antibody
    • Cep110 antibody
    • CP110 antibody
    • CP110_HUMAN antibody
    • DKFZp781G1416 antibody
    • FAN antibody
    • KIAA0419 antibody
    see all

Images

  • All lanes : Anti-CP110 antibody (ab99337) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 113 kDa


    Exposure time: 3 minutes
  • ab99337 at 1µg/ml detecting CP110 in HeLa whole cell lysate by WB following IP.

    Lane 1: ab99337 IP at 3µg/mg of lysate
    Lane 2: IP with an antibody which recognizes an downstream epitope of CP110
    Lane 3: control IgG.

    In each case, 1 mg of lysate was used for IP and 20% of the IP was loaded.
    Detection: Chemiluminescence with an exposure time of 30 seconds.

References

This product has been referenced in:

  • Rowlison T  et al. Deciphering the mechanisms involving cenexin, ninein and centriolin in sperm maturation during epididymal transit in the domestic cat. Reprod Domest Anim 52 Suppl 2:193-196 (2017). Read more (PubMed: 27807895) »
  • Hori A  et al. Centriolar satellite- and hMsd1/SSX2IP-dependent microtubule anchoring is critical for centriole assembly. Mol Biol Cell 26:2005-19 (2015). ICC/IF ; Human . Read more (PubMed: 25833712) »
See all 2 Publications for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Answer

Thank you for your response.

I am not sure what IB represents but assume the customer would mean either IP (Immunoprecipitation) or WB (Western blot) application? Is that correct?

I regret to inform you that I am not convinced that this antibody (ab99337) is faulty. However, I appreciate your long-term relationship with this customer and try to help you to close this case soon.

Here are a few comments I would like to make and share:

Multiple bands could be due to several factors (blocking, protein degradation, secondary antibody). Since the non-specific bands are lower range than the expected band size for CP110, it may well be that the bands are degradation product.

Unfortunately, I have limited information about the experiments but in future in similar complaint the following details would be advised to provide in order to get sufficient technical support

What Lane 1 and 2 represent? Identical samples? Or different preparation?
How was the blocking applied (blocking agent, time and temperature)?
How much protein was loaded?
Has "No primary" (only secondary) antibody control was used to rule out any non-specific signal due to the secondary antibody?



Please be advised that there are two isoforms (O43303-1 and O43303-2) which have been described in the literature and it would be advisable to check which one the customer’s samples express. Your customer could find some further information about this target at this site:

http://www.uniprot.org/uniprot/O43303

Furthermore, CCP110 has different subunits and the CCP110 protein can interact with other proteins such CALM1, CETN2, CEP76, CEP97, NEURL4 and CCNF. For this reason, it is essential to know if the experiments were carried out under naturing or denaturing/and reducing conditions. This information is also missing in the last e-mail and would be very important to know.

Currently, we have two alternative antibodies against CP110 ab99338 and ab91415 and can offer one free of charge replacement vial. Could you please discuss the content of this e-mail with your customer and you let me know which product he wishes to test.

Thank you for your continued cooperation.

Read More

Answer

Thank you for your response.

Unfortunately, the two image files can't be opened so I am unable to take a look at the signal.

Tested and Guaranted Applications:

ab11257 - WB, ICC/IF

ab99337 - WB and IP - this has not been tested in immunostaining.

Since ab99337 has not been tested in immunostaining, I am very sorry but our Abpromise does not apply for this antibody.

Regarding ab11257, the protocol looks fine though it would be interesting to know if the cultured HeLa cells are from the exponential phase of growth or from a confluent culture. No information is provided. Furthermore, blocking non-specific bindings with 10% BSA may be a bit too strong with cultured cells.

I can offer a new free of charge vial of 11257 but not for the other product ab99337 which has not been tested for ICC/IF yet.

Could you please discuss our policy with the customer to make sure he understands for which product we can offer the Abpromise.

I look forward to hearing from you soon.

Read More

Answer

Thank you for your enquiry regarding our two products. I am very sorry to hear that you are having problems with this antibody.

As our Abpromise indicates, in the event that a product is not functioning in the applications/species cited on the product data sheet (and the problem has been reported within 6 months of purchase) we will happily offer a credit note/refund to the value of the product purchased.

Tested and Guaranted Applications:

ab11257 - WB, ICC/IF

ab99337 - WB and IP - this has not been tested in immunostaining

Could you please provide some details of the protocol used and complete the following form (attached as a word document). It would be much appreciated if you could attach an image to the response.

- Batch/Order numbers,

- Species and type of the sample,

- Cells or tissue section used,

- Fixation method

Thank you for your understanding and co-operation in this matter. I look forward to hearing from you soon and resolving this issue as soon as possible.

Read More

Answer

Thank you for contacting us and reporting the problems encountered when using anti-CP110 antibody (ab99337).

I am sorry to hear you are experiencing difficulties with one of our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

In order to understand the situation more fully could you please fill out the questionnaire I have attached to this email. This should not take more than 5-10 minutesand theinformation provided willallowme to investigate this case internally and initiate additional testing where necessary. If you would be able to share images ofthe blots youhave obtained with ab99337 and thatof theother antibodyused that would be very helpful.

If the product was purchased in the last six monthsand is being used according to our Abpromise, we would be happy to replace or refund the antibody.

I look forward to receiving your reply.

Read More

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