Product nameAnti-CP110 antibody
See all CP110 primary antibodies
DescriptionRabbit polyclonal to CP110
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Pig, Chimpanzee, Ferret, Rhesus monkey, Gorilla
Synthetic peptide, corresponding to a region within amino acids within residues 575-625 of Human CP110 (CAH18111.1).
- HeLa and 293T whole cell lysates
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab99337 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 113 kDa.|
|IP||Use at 2-5 µg/mg of lysate.|
FunctionNecessary for centrosome duplication at different stages of procentriole formation. Collaborates with CEP97, being involved in the suppression of a cilia assembly program. Required for correct spindle formation and has a role in regulating cytokinesis and genome stability via cooperation with CALM1 and CETN2.
Tissue specificityHighly expressed in testis. Detected at intermediate levels in spleen, thymus, prostate, small intestine, colon and peripheral blood leukocytes.
modificationsPhosphorylated by CDKs.
Ubiquitinated by the SCF(Cyclin F) during G2 phase, leading to its degradation by the proteasome and preventing centrosome reduplication.
Cellular localizationCytoplasm > cytoskeleton > centrosome > centriole. Recruited early and then associates with the growing distal tips.
- Information by UniProt
- 110 kDa centrosomal protein antibody
- CCP110 antibody
- Centriolar coiled coil protein 110kDa antibody
All lanes : Anti-CP110 antibody (ab99337) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 113 kDa
Exposure time: 3 minutes
ab99337 at 1µg/ml detecting CP110 in HeLa whole cell lysate by WB following IP.
Lane 1: ab99337 IP at 3µg/mg of lysate
Lane 2: IP with an antibody which recognizes an downstream epitope of CP110
Lane 3: control IgG.
In each case, 1 mg of lysate was used for IP and 20% of the IP was loaded.
Detection: Chemiluminescence with an exposure time of 30 seconds.
This product has been referenced in:
- Rowlison T et al. Deciphering the mechanisms involving cenexin, ninein and centriolin in sperm maturation during epididymal transit in the domestic cat. Reprod Domest Anim 52 Suppl 2:193-196 (2017). Read more (PubMed: 27807895) »
- Hori A et al. Centriolar satellite- and hMsd1/SSX2IP-dependent microtubule anchoring is critical for centriole assembly. Mol Biol Cell 26:2005-19 (2015). ICC/IF ; Human . Read more (PubMed: 25833712) »