CPD ELISA Kit (cyclobutane pyrimidine dimers, DNA samples) (ab242297)
- Datasheet
- References
- Protocols
Overview
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Product name
CPD ELISA Kit (cyclobutane pyrimidine dimers, DNA samples)
See all DNA damage kits -
Assay type
Cell-based (quantitative) -
Product overview
CPD ELISA Kit (ab242297) is developed for rapid detection and quantitation of CPD in any DNA samples.
The quantity of CPD in unknown sample is determined by comparing its absorbance with that of a known CPD-DNA standard curve. Each kit provides sufficient reagents to perform up to 96 assays, including standard curve and unknown samples.
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Tested applications
Suitable for: ELISAmore details -
Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 32 tests 10X Wash Buffer 1 x 100ml 1 x 30ml Anti-CPD Antibody 1 x 20µl 1 x 10µl Assay Diluent 1 x 50ml 1 x 20ml CPD-DNA Standard 1 x 100µl 1 x 40µl DNA Binding Solution 1 x 6ml 2 x 1.25ml DNA High-Binding Plate 1 unit 1 unit Reduced DNA Standard 1 x 100µl 1 x 40µl Secondary Antibody, HRP Conjugate 1 x 50µl 1 x 50µl Stop Solution 1 x 12ml 1 x 4ml Substrate Solution 1 x 12ml 1 x 4ml -
Research areas
Applications
Our Abpromise guarantee covers the use of ab242297 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ELISA | Use at an assay dependent concentration. |
Images
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CPD-DNA Standard Curve. -
DNA Damage Induced by UV Light.0.2 mg/mL Calf thymus DNA was exposed to UV light inside a cell culture hood for the time indicated. The CPD levels in denatured DNA samples were determined as described in the Assay Protocol.
Protocols
References
ab242297 has not yet been referenced specifically in any publications.
