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Epigenetics and Nuclear Signaling DNA / RNA DNA Damage & Repair Nucl. Excision Repair
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CPD ELISA Kit (cyclobutane pyrimidine dimers, DNA samples) (ab242297)

  • Datasheet
  • SDS
  • Protocol Booklet
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CPD-DNA Standard Curve.
  • DNA Damage Induced by UV Light.

Key features and details

  • Assay type: Cell-based (quantitative)
  • Platform: Microplate

Overview

  • Product name

    CPD ELISA Kit (cyclobutane pyrimidine dimers, DNA samples)
    See all DNA damage kits
  • Assay type

    Cell-based (quantitative)
  • Product overview

    CPD ELISA Kit (ab242297) is developed for rapid detection and quantitation of CPD in any DNA samples.


    The quantity of CPD in unknown sample is determined by comparing its absorbance with that of a known CPD-DNA standard curve. Each kit provides sufficient reagents to perform up to 96 assays, including standard curve and unknown samples.

  • Tested applications

    Suitable for: ELISAmore details
  • Platform

    Microplate

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Wash Buffer 1 x 100ml
    Anti-CPD Antibody 1 x 20µl
    Assay Diluent 1 x 50ml
    CPD-DNA Standard 1 x 100µl
    DNA Binding Solution 1 x 6ml
    DNA High-Binding Plate 1 unit
    Reduced DNA Standard 1 x 100µl
    Secondary Antibody, HRP Conjugate 1 x 50µl
    Stop Solution 1 x 12ml
    Substrate Solution 1 x 12ml
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA Damage & Repair
    • Nucl. Excision Repair
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA Damage & Repair
    • Base Excision Repair
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA Damage & Repair
    • DNA Damage Response
    • Other
    • Kits/ Lysates/ Other
    • Kits
    • Cell Damage Kits
    • Cell Damage

Applications

Our Abpromise guarantee covers the use of ab242297 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.

Images

  • CPD-DNA Standard Curve.
    CPD-DNA Standard Curve.
  • DNA Damage Induced by UV Light.
    DNA Damage Induced by UV Light.

    0.2 mg/mL Calf thymus DNA was exposed to UV light inside a cell culture hood for the time indicated. The CPD levels in denatured DNA samples were determined as described in the Assay Protocol.

Protocols

  • Protocol Booklet

Click here to view the general protocols

Datasheets and documents

    • Datasheet
    • SDS
  • References (0)

    Publishing research using ab242297? Please let us know so that we can cite the reference in this datasheet.

    ab242297 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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