Overview

  • Product name

  • Description

    Rabbit polyclonal to CPEB1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 239-417 of Human CPEB1 (UniProt Q9BZB8).

  • Positive control

    • NT2D1, IMR32, U-87 MG and MCF7 whole cell lysates; Human colon carcinoma tissue.
  • General notes

    Keep as concentrated solution.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 78% PBS, 20% Glycerol, 1% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab127739 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 54, 63 kDa.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Sequence-specific RNA-binding protein that regulates mRNA cytoplasmic polyadenylation and translation initiation during oocyte maturation, early development and at postsynapse sites of neurons. Binds to the cytoplasmic polyadenylation element (CPE), an uridine-rich sequence element (consensus sequence 5'-UUUUUAU-3') within the mRNA 3'-UTR. In absence of phosphorylation and in association with TACC3 is also involved as a repressor of translation of CPE-containing mRNA; a repression that is relieved by phosphorylation or degradation (By similarity). Involved in the transport of CPE-containing mRNA to dendrites; those mRNAs may be transported to dendrites in a translationally dormant form and translationally activated at synapses (By similarity). Its interaction with APLP1 promotes local CPE-containing mRNA polyadenylation and translation activation (By similarity). Induces the assembly of stress granules in the absence of stress.
  • Tissue specificity

    Isoform 1 is expressed in immature oocytes, ovary, brain and heart. Isoform 2 is expressed in brain and heart. Isoform 3 and isoform 4 are expressed in brain. Expressed in breast tumors and several tumor cell lines.
  • Sequence similarities

    Belongs to the RRM CPEB family.
    Contains 2 RRM (RNA recognition motif) domains.
  • Domain

    The 2 RRM domains and the C-terminal region mediate interaction with CPE-containing RNA.
  • Post-translational
    modifications

    Phosphorylated on serine/threonine residues by AURKA/STK6 within positions 166 and 197. Phosphorylation and dephosphorylation on Thr-172 regulates cytoplasmic polyadenylation and translation of CPE-containing mRNAs. Phosphorylation on Thr-172 by AURKA/STK6 and CAMK2A activates CPEB1. Phosphorylation on Thr-172 may be promoted by APLP1. Phosphorylation increases binding to RNA.
  • Cellular localization

    Cytoplasm > P-body. Cytoplasmic granule. Cell junction > synapse. Membrane. Cell junction > synapse > postsynaptic cell membrane > postsynaptic density. Cell projection > dendrite. Also found in stress granules. Recruited to stress granules (SGs) upon arsenite treatment. In dendrites (By similarity). Localizes in synaptosomes at dendritic synapses of neurons (By similarity). Strongly enriched in postsynaptic density (PSD) fractions (By similarity). Transported into dendrites in a microtubule-dependent fashion and colocalizes in mRNA-containing particles with TACC3, dynein and kinesin (By similarity). Membrane-associated (By similarity). Colocalizes at excitatory synapses with members of the polyadenylation and translation complex factors (CPSF, APLP1, TACC3, AURKA/STK6, SYP, etc.) including CPE-containing RNAs.
  • Information by UniProt
  • Database links

  • Alternative names

    • CEBP antibody
    • CPE binding protein 1 antibody
    • CPE BP1 antibody
    • CPE-binding protein 1 antibody
    • CPE-BP1 antibody
    • CPEB 1 antibody
    • CPEB antibody
    • CPEB-1 antibody
    • CPEB1 antibody
    • CPEB1 protein antibody
    • CPEB1_HUMAN antibody
    • Cytoplasmic polyadenylation binding protein 1 antibody
    • Cytoplasmic polyadenylation element binding protein antibody
    • Cytoplasmic polyadenylation element binding protein 1 antibody
    • Cytoplasmic polyadenylation element-binding protein 1 antibody
    • FLJ13203 antibody
    • h CEBP antibody
    • h-CEBP antibody
    • hCPEB 1 antibody
    • hCPEB-1 antibody
    • MGC34136 antibody
    • MGC60106 antibody
    • mKIAA0940 antibody
    • MKIAA0940 protein antibody
    see all

Images

  • Anti-CPEB1 antibody (ab127739) at 1/1000 dilution + U-87 MG whole cell lysate at 30 µg

    Predicted band size: 54, 63 kDa



    10% SDS PAGE
  • ab127739, at a dilution of 1/250, staining CPEB1 in paraffin-embedded Human colon carcinoma tissue by Immunohistochemistry.

References

ab127739 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Sample
Rat Tissue lysate - whole (LGN)
Loading amount
1000 µg
Specification
LGN
Gel Running Conditions
Reduced Denaturing (MINI-PROTEAN TGX)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Dr. Wanhua Shen

Verified customer

Submitted Mar 20 2012

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