Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.05% Tris buffered saline, 0.5% BSA
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab15917 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use at a concentration of 0.5 - 2.0 µg/ml. Predicted molecular weight: 65 kDa. (65-70kDa band observed in Human Brain lysates. Please note this band was always accompanied by a slightly stonger band at approx. 48kDa and both bands could be blocked by incubation with the immunising peptide).
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionSequence-specific RNA-binding protein that regulates mRNA cytoplasmic polyadenylation and translation initiation during oocyte maturation, early development and at postsynapse sites of neurons. Binds to the cytoplasmic polyadenylation element (CPE), an uridine-rich sequence element (consensus sequence 5'-UUUUUAU-3') within the mRNA 3'-UTR. In absence of phosphorylation and in association with TACC3 is also involved as a repressor of translation of CPE-containing mRNA; a repression that is relieved by phosphorylation or degradation (By similarity). Involved in the transport of CPE-containing mRNA to dendrites; those mRNAs may be transported to dendrites in a translationally dormant form and translationally activated at synapses (By similarity). Its interaction with APLP1 promotes local CPE-containing mRNA polyadenylation and translation activation (By similarity). Induces the assembly of stress granules in the absence of stress.
Tissue specificityIsoform 1 is expressed in immature oocytes, ovary, brain and heart. Isoform 2 is expressed in brain and heart. Isoform 3 and isoform 4 are expressed in brain. Expressed in breast tumors and several tumor cell lines.
Sequence similaritiesBelongs to the RRM CPEB family.
Contains 2 RRM (RNA recognition motif) domains.
DomainThe 2 RRM domains and the C-terminal region mediate interaction with CPE-containing RNA.
modificationsPhosphorylated on serine/threonine residues by AURKA/STK6 within positions 166 and 197. Phosphorylation and dephosphorylation on Thr-172 regulates cytoplasmic polyadenylation and translation of CPE-containing mRNAs. Phosphorylation on Thr-172 by AURKA/STK6 and CAMK2A activates CPEB1. Phosphorylation on Thr-172 may be promoted by APLP1. Phosphorylation increases binding to RNA.
Cellular localizationCytoplasm > P-body. Cytoplasmic granule. Cell junction > synapse. Membrane. Cell junction > synapse > postsynaptic cell membrane > postsynaptic density. Cell projection > dendrite. Also found in stress granules. Recruited to stress granules (SGs) upon arsenite treatment. In dendrites (By similarity). Localizes in synaptosomes at dendritic synapses of neurons (By similarity). Strongly enriched in postsynaptic density (PSD) fractions (By similarity). Transported into dendrites in a microtubule-dependent fashion and colocalizes in mRNA-containing particles with TACC3, dynein and kinesin (By similarity). Membrane-associated (By similarity). Colocalizes at excitatory synapses with members of the polyadenylation and translation complex factors (CPSF, APLP1, TACC3, AURKA/STK6, SYP, etc.) including CPE-containing RNAs.
- Information by UniProt
- CEBP antibody
- CPE binding protein 1 antibody
- CPE BP1 antibody
Anti-CPEB1 antibody (ab15917) at 1/0.5 dilution + Human Brain lysate at 35 µg
Predicted band size: 65 kDa
Observed band size: 65 kDa
Additional bands at: 49 kDa (possible cross reactivity, but this band is not blocked)
Exposure time: 1 hour
The additional band shown at approx 49KDa can be blocked using the immunising peptide.
ab15917 has not yet been referenced specifically in any publications.