Key features and details
- Rabbit polyclonal to CPEB1
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-CPEB1 antibody
See all CPEB1 primary antibodies
DescriptionRabbit polyclonal to CPEB1
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Human
A 14 amino acid peptide from near the amino terminus of human CPEB1.
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferpH: 7.2
Preservative: 0.02% Sodium azide
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab73287 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 63 kDa (predicted molecular weight: 63 kDa).|
|ICC/IF||Use a concentration of 5 µg/ml.|
FunctionSequence-specific RNA-binding protein that regulates mRNA cytoplasmic polyadenylation and translation initiation during oocyte maturation, early development and at postsynapse sites of neurons. Binds to the cytoplasmic polyadenylation element (CPE), an uridine-rich sequence element (consensus sequence 5'-UUUUUAU-3') within the mRNA 3'-UTR. In absence of phosphorylation and in association with TACC3 is also involved as a repressor of translation of CPE-containing mRNA; a repression that is relieved by phosphorylation or degradation (By similarity). Involved in the transport of CPE-containing mRNA to dendrites; those mRNAs may be transported to dendrites in a translationally dormant form and translationally activated at synapses (By similarity). Its interaction with APLP1 promotes local CPE-containing mRNA polyadenylation and translation activation (By similarity). Induces the assembly of stress granules in the absence of stress.
Tissue specificityIsoform 1 is expressed in immature oocytes, ovary, brain and heart. Isoform 2 is expressed in brain and heart. Isoform 3 and isoform 4 are expressed in brain. Expressed in breast tumors and several tumor cell lines.
Sequence similaritiesBelongs to the RRM CPEB family.
Contains 2 RRM (RNA recognition motif) domains.
DomainThe 2 RRM domains and the C-terminal region mediate interaction with CPE-containing RNA.
modificationsPhosphorylated on serine/threonine residues by AURKA/STK6 within positions 166 and 197. Phosphorylation and dephosphorylation on Thr-172 regulates cytoplasmic polyadenylation and translation of CPE-containing mRNAs. Phosphorylation on Thr-172 by AURKA/STK6 and CAMK2A activates CPEB1. Phosphorylation on Thr-172 may be promoted by APLP1. Phosphorylation increases binding to RNA.
Cellular localizationCytoplasm > P-body. Cytoplasmic granule. Cell junction > synapse. Membrane. Cell junction > synapse > postsynaptic cell membrane > postsynaptic density. Cell projection > dendrite. Also found in stress granules. Recruited to stress granules (SGs) upon arsenite treatment. In dendrites (By similarity). Localizes in synaptosomes at dendritic synapses of neurons (By similarity). Strongly enriched in postsynaptic density (PSD) fractions (By similarity). Transported into dendrites in a microtubule-dependent fashion and colocalizes in mRNA-containing particles with TACC3, dynein and kinesin (By similarity). Membrane-associated (By similarity). Colocalizes at excitatory synapses with members of the polyadenylation and translation complex factors (CPSF, APLP1, TACC3, AURKA/STK6, SYP, etc.) including CPE-containing RNAs.
- Information by UniProt
- CEBP antibody
- CPE binding protein 1 antibody
- CPE BP1 antibody
Lane 1 : Anti-CPEB1 antibody (ab73287) at 1 µg/ml
Lane 2 : Anti-CPEB1 antibody (ab73287) at 2 µg/ml
All lanes : rat brain tissue lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 63 kDa
Observed band size: 63 kDa
ICC/IF image of ab73287 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab73287, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
ab73287 has been referenced in 5 publications.
- Daldello EM et al. Cyclin B2 is required for progression through meiosis in mouse oocytes. Development 146:N/A (2019). PubMed: 30952665
- Han SJ et al. The Translation of Cyclin B1 and B2 is Differentially Regulated during Mouse Oocyte Reentry into the Meiotic Cell Cycle. Sci Rep 7:14077 (2017). PubMed: 29074977
- Baj G et al. Signaling pathways controlling activity-dependent local translation of BDNF and their localization in dendritic arbors. J Cell Sci 129:2852-64 (2016). PubMed: 27270670
- Sousa Martins JP et al. DAZL and CPEB1 regulate mRNA translation synergistically during oocyte maturation. J Cell Sci 129:1271-82 (2016). PubMed: 26826184
- Alves-Sampaio A et al. NMDA-mediated regulation of DSCAM dendritic local translation is lost in a mouse model of Down's syndrome. J Neurosci 30:13537-48 (2010). RIP ; Mouse . PubMed: 20926679