Anti-CPEB1 antibody (ab73287)
Key features and details
- Rabbit polyclonal to CPEB1
- Suitable for: WB, ICC/IF
- Reacts with: Rat
- Isotype: IgG
Overview
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Product name
Anti-CPEB1 antibody
See all CPEB1 primary antibodies -
Description
Rabbit polyclonal to CPEB1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Rat -
Immunogen
A 14 amino acid peptide from near the amino terminus of human CPEB1.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab73287 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 63 kDa (predicted molecular weight: 63 kDa).
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ICC/IF |
Use a concentration of 5 µg/ml.
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Notes |
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WB
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 63 kDa (predicted molecular weight: 63 kDa). |
ICC/IF
Use a concentration of 5 µg/ml. |
Target
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Function
Sequence-specific RNA-binding protein that regulates mRNA cytoplasmic polyadenylation and translation initiation during oocyte maturation, early development and at postsynapse sites of neurons. Binds to the cytoplasmic polyadenylation element (CPE), an uridine-rich sequence element (consensus sequence 5'-UUUUUAU-3') within the mRNA 3'-UTR. In absence of phosphorylation and in association with TACC3 is also involved as a repressor of translation of CPE-containing mRNA; a repression that is relieved by phosphorylation or degradation (By similarity). Involved in the transport of CPE-containing mRNA to dendrites; those mRNAs may be transported to dendrites in a translationally dormant form and translationally activated at synapses (By similarity). Its interaction with APLP1 promotes local CPE-containing mRNA polyadenylation and translation activation (By similarity). Induces the assembly of stress granules in the absence of stress. -
Tissue specificity
Isoform 1 is expressed in immature oocytes, ovary, brain and heart. Isoform 2 is expressed in brain and heart. Isoform 3 and isoform 4 are expressed in brain. Expressed in breast tumors and several tumor cell lines. -
Sequence similarities
Belongs to the RRM CPEB family.
Contains 2 RRM (RNA recognition motif) domains. -
Domain
The 2 RRM domains and the C-terminal region mediate interaction with CPE-containing RNA. -
Post-translational
modificationsPhosphorylated on serine/threonine residues by AURKA/STK6 within positions 166 and 197. Phosphorylation and dephosphorylation on Thr-172 regulates cytoplasmic polyadenylation and translation of CPE-containing mRNAs. Phosphorylation on Thr-172 by AURKA/STK6 and CAMK2A activates CPEB1. Phosphorylation on Thr-172 may be promoted by APLP1. Phosphorylation increases binding to RNA. -
Cellular localization
Cytoplasm > P-body. Cytoplasmic granule. Cell junction > synapse. Membrane. Cell junction > synapse > postsynaptic cell membrane > postsynaptic density. Cell projection > dendrite. Also found in stress granules. Recruited to stress granules (SGs) upon arsenite treatment. In dendrites (By similarity). Localizes in synaptosomes at dendritic synapses of neurons (By similarity). Strongly enriched in postsynaptic density (PSD) fractions (By similarity). Transported into dendrites in a microtubule-dependent fashion and colocalizes in mRNA-containing particles with TACC3, dynein and kinesin (By similarity). Membrane-associated (By similarity). Colocalizes at excitatory synapses with members of the polyadenylation and translation complex factors (CPSF, APLP1, TACC3, AURKA/STK6, SYP, etc.) including CPE-containing RNAs. - Information by UniProt
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Database links
- Entrez Gene: 293056 Rat
- SwissProt: P0C279 Rat
- Unigene: 224485 Rat
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Alternative names
- CEBP antibody
- CPE binding protein 1 antibody
- CPE BP1 antibody
see all
Images
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Lane 1 : Anti-CPEB1 antibody (ab73287) at 1 µg/ml
Lane 2 : Anti-CPEB1 antibody (ab73287) at 2 µg/ml
All lanes : rat brain tissue lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 63 kDa
Observed band size: 63 kDa -
ICC/IF image of ab73287 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab73287, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (13)
ab73287 has been referenced in 13 publications.
- Oe S et al. Cpeb1 expression is post-transcriptionally regulated by AUF1, CPEB1, and microRNAs. FEBS Open Bio 12:82-94 (2022). PubMed: 34480525
- Hu Q et al. Obesity-Induced miR-455 Upregulation Promotes Adaptive Pancreatic β-Cell Proliferation Through the CPEB1/CDKN1B Pathway. Diabetes 71:394-411 (2022). PubMed: 35029277
- Zeng W et al. CPEB1 directs muscle stem cell activation by reprogramming the translational landscape. Nat Commun 13:947 (2022). PubMed: 35177647
- Oe S et al. Cytoplasmic Polyadenylation Element-Binding Protein 1 Post-transcriptionally Regulates Fragile X Mental Retardation 1 Expression Through 3' Untranslated Region in Central Nervous System Neurons. Front Cell Neurosci 16:869398 (2022). PubMed: 35496917
- Aboelenain M & Schindler K Aurora kinase B inhibits aurora kinase A to control maternal mRNA translation in mouse oocytes. Development 148:N/A (2021). PubMed: 34636397