Overview

  • Product name

    Anti-CPEB1 antibody [EPR11775(2)]
    See all CPEB1 primary antibodies
  • Description

    Rabbit monoclonal [EPR11775(2)] to CPEB1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human CPEB1 aa 250-350. The exact sequence is proprietary.
    Database link: Q9BZB8
    (Peptide available as ab197860)

  • Positive control

    • Human fetal brain, fetal heart, HeLa and A549 cell lysates.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, 0.05% BSA, 59% PBS
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR11775(2)
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab181051 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/2000. Detects a band of approximately 70 kDa (predicted molecular weight: 63 kDa).Can be blocked with CPEB1 peptide (ab197860).
IP 1/60.

Target

  • Function

    Sequence-specific RNA-binding protein that regulates mRNA cytoplasmic polyadenylation and translation initiation during oocyte maturation, early development and at postsynapse sites of neurons. Binds to the cytoplasmic polyadenylation element (CPE), an uridine-rich sequence element (consensus sequence 5'-UUUUUAU-3') within the mRNA 3'-UTR. In absence of phosphorylation and in association with TACC3 is also involved as a repressor of translation of CPE-containing mRNA; a repression that is relieved by phosphorylation or degradation (By similarity). Involved in the transport of CPE-containing mRNA to dendrites; those mRNAs may be transported to dendrites in a translationally dormant form and translationally activated at synapses (By similarity). Its interaction with APLP1 promotes local CPE-containing mRNA polyadenylation and translation activation (By similarity). Induces the assembly of stress granules in the absence of stress.
  • Tissue specificity

    Isoform 1 is expressed in immature oocytes, ovary, brain and heart. Isoform 2 is expressed in brain and heart. Isoform 3 and isoform 4 are expressed in brain. Expressed in breast tumors and several tumor cell lines.
  • Sequence similarities

    Belongs to the RRM CPEB family.
    Contains 2 RRM (RNA recognition motif) domains.
  • Domain

    The 2 RRM domains and the C-terminal region mediate interaction with CPE-containing RNA.
  • Post-translational
    modifications

    Phosphorylated on serine/threonine residues by AURKA/STK6 within positions 166 and 197. Phosphorylation and dephosphorylation on Thr-172 regulates cytoplasmic polyadenylation and translation of CPE-containing mRNAs. Phosphorylation on Thr-172 by AURKA/STK6 and CAMK2A activates CPEB1. Phosphorylation on Thr-172 may be promoted by APLP1. Phosphorylation increases binding to RNA.
  • Cellular localization

    Cytoplasm > P-body. Cytoplasmic granule. Cell junction > synapse. Membrane. Cell junction > synapse > postsynaptic cell membrane > postsynaptic density. Cell projection > dendrite. Also found in stress granules. Recruited to stress granules (SGs) upon arsenite treatment. In dendrites (By similarity). Localizes in synaptosomes at dendritic synapses of neurons (By similarity). Strongly enriched in postsynaptic density (PSD) fractions (By similarity). Transported into dendrites in a microtubule-dependent fashion and colocalizes in mRNA-containing particles with TACC3, dynein and kinesin (By similarity). Membrane-associated (By similarity). Colocalizes at excitatory synapses with members of the polyadenylation and translation complex factors (CPSF, APLP1, TACC3, AURKA/STK6, SYP, etc.) including CPE-containing RNAs.
  • Information by UniProt
  • Database links

  • Alternative names

    • CEBP antibody
    • CPE binding protein 1 antibody
    • CPE BP1 antibody
    • CPE-binding protein 1 antibody
    • CPE-BP1 antibody
    • CPEB 1 antibody
    • CPEB antibody
    • CPEB-1 antibody
    • CPEB1 antibody
    • CPEB1 protein antibody
    • CPEB1_HUMAN antibody
    • Cytoplasmic polyadenylation binding protein 1 antibody
    • Cytoplasmic polyadenylation element binding protein antibody
    • Cytoplasmic polyadenylation element binding protein 1 antibody
    • Cytoplasmic polyadenylation element-binding protein 1 antibody
    • FLJ13203 antibody
    • h CEBP antibody
    • h-CEBP antibody
    • hCPEB 1 antibody
    • hCPEB-1 antibody
    • MGC34136 antibody
    • MGC60106 antibody
    • mKIAA0940 antibody
    • MKIAA0940 protein antibody
    see all

Images

  • All lanes : Anti-CPEB1 antibody [EPR11775(2)] (ab181051) at 1/1000 dilution

    Lane 1 : Human fetal brain lysate
    Lane 2 : Human fetal heart lysate
    Lane 3 : HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/500 dilution

    Predicted band size: 63 kDa

  • Anti-CPEB1 antibody [EPR11775(2)] (ab181051) at 1/1000 dilution + A549 cell lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 63 kDa

  • Western blot analysis of immunoprecipitation pellet from Human fetal brain lysate immunoprecipitated using ab181051 at 1/60 dilution.
    Secondary: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution.

References

ab181051 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing (8% SDS -PAGE)
Sample
Mouse Tissue lysate - whole (testes)
Specification
testes
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

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Verified customer

Submitted Feb 12 2015

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