Product nameAnti-CPEB3 antibody
DescriptionRabbit polyclonal to CPEB3
Tested applicationsSuitable for: IHC-Fr, IHC-FoFr, WB, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat
Predicted to work with: Human
Synthetic peptide derived from residues 300 - 400 of Mouse CPEB3.
- This antibody gave a positive signal when tested against Mouse Brain Tissue. This antibody gave a positive signal in the following cell types: SKNSH.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
PurityImmunogen affinity purified
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab10883 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||Use at an assay dependent concentration.|
|WB||1/500 - 1/1000. Detects a band of approximately 75 kDa (predicted molecular weight: 75 kDa).|
|ICC/IF||Use a concentration of 5 µg/ml.|
Sequence similaritiesBelongs to the RRM CPEB family.
Contains 2 RRM (RNA recognition motif) domains.
- Information by UniProt
- CPE-binding protein 3 antibody
- CPE-BP3 antibody
- CPEB 3 antibody
ab10883 staining CPEB3 in mouse brain tissue section by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue from 4% PFA perfused animals underwent overnight fixation in 4% paraformaldehyde, cryoprotected in 30% sucrose and cut using cryostat.The primary antibody was diluted, 1/100 (PBS + 0.3% Triton X100) and incubated with sample for 18 hours at 20°C. An Alexa Fluor®488 conjugated goat polyclonal to rabbit IgG at 1/1000 dilution, was used as secondary.
ICC/IF image of ab10883 stained SKNSH cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab10883 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Lane 1 : Anti-CPEB3 antibody (ab10883) at 1/500 dilution
Lane 2 : Anti-CPEB3 antibody (ab10883) at 1/1000 dilution
All lanes : Mouse Brain lysate
Lysates/proteins at 5 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab7090) at 1/5000 dilution
Predicted band size: 75 kDa
Exposure time: 3 minutes
Lanes 1 to 2
Lane 1: 5
g per lane Mouse Brain 1/500 µ
Lane 2: 5
g per lane Mouse Brain 1/1000 µ
Exposure time: 3 minutes
Expected molecular weight: 78kDa
Secondary ab: Goat polyclonal to Rabbit IgG H&L (HRP) Pre-Adsorbed
We think that the top band here is the correct band, as it is the same size as the most intense band on the previous blot. The banding pattern is the same on both blots and the lower bands may be clevage products as there is no evidence of CPEB3 isoforms in the literature.We think that the top band here is the correct band, as it is the same size as the most intense band on the previous blot. The banding pattern is the same on both blots and the lower bands may be clevage products as there is no evidence of CPEB3 isoforms in the literature.
This product has been referenced in:
- Kaczmarczyk L et al. New Phosphospecific Antibody Reveals Isoform-Specific Phosphorylation of CPEB3 Protein. PLoS One 11:e0150000 (2016). WB . Read more (PubMed: 26915047) »
- Burguete AS et al. GGGGCC microsatellite RNA is neuritically localized, induces branching defects, and perturbs transport granule function. Elife 4:e08881 (2015). Read more (PubMed: 26650351) »