Product nameAnti-CPEB4 antibody - N-terminal
See all CPEB4 primary antibodies
DescriptionRabbit polyclonal to CPEB4 - N-terminal
Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Recombinant fragment, corresponding to a region within N terminal amino acids 1-172 of Human CPEB4 (UniProt ID: Q17RY0).
- NT2D1, PC3, U87 MG, SK-N-SH whole cell lysate; U87 xenograft This antibody gave a positive result when used in the following methanol fixed cell lines: HeLa
General notesKeep as a concentrated solution.
Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78% PBS, 20% Glycerol, 1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab155204 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 5 µg/ml.|
|WB||1/5000 - 1/20000. Predicted molecular weight: 80 kDa.|
|IHC-P||1/100 - 1/1000.|
Sequence similaritiesBelongs to the RRM CPEB family.
Contains 2 RRM (RNA recognition motif) domains.
- Information by UniProt
- CPE binding protein 4 antibody
- CPE-binding protein 4 antibody
- CPE-BP4 antibody
All lanes : Anti-CPEB4 antibody - N-terminal (ab155204) at 1/10000 dilution
Lane 1 : NT2D1 whole cell lysate
Lane 2 : PC3 whole cell lysate
Lane 3 : U87-MG whole cell lysate
Lane 4 : Sk-N-SH whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 80 kDa
7.5% SDS PAGE
ICC/IF image of ab155204 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab155204 at 5µg/ml overnight at +4°C. The secondary antibody (pseudo-colored green) was Dylight® 488 goat anti- rabbit (ab96899) IgG (H+L) preadsorbed, used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.
Immunohistochemical analysis of paraffin embedded U87 xenograft tissue labeling CPEB4 with ab155204 at 1/500 dilution.
ab155204 has not yet been referenced specifically in any publications.