Key features and details
- Rabbit polyclonal to cPLA2 beta
- Suitable for: WB, ELISA
- Reacts with: Human
- Isotype: IgG
Product nameAnti-cPLA2 beta antibody
See all cPLA2 beta primary antibodies
DescriptionRabbit polyclonal to cPLA2 beta
Tested applicationsSuitable for: WB, ELISAmore details
Species reactivityReacts with: Human
Recombinant full length protein without the signal sequence and activation peptide, (Human) with an additional 16 residue histidine tag
Storage instructionsShipped at 4°C. Upon delivery aliquot. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Constituents: 0.58% Sodium chloride, 0.134% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab53421 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 114 kDa.|
|ELISA||Use at an assay dependent dilution.|
RelevanceThis gene encodes a member of the cytosolic phospholipase A2 protein family. Phospholipase A2 enzymes hydrolyze the sn-2 bond of phospholipids, releasing lysophospholipids and fatty acids. This enzyme may be associated with mitochondria and early endosomes. Most tissues also express read-through transcripts from the upstream gene into this gene, some of which may encode fusion proteins combining the N-terminus of the upstream gene including its JmjC domain with the almost complete coding region of this gene, including the C2 and cytoplasmic phospholipase A2 domains.
Cellular localizationCytoplasmic and Mitochondrial
- CPLA2 beta antibody
- Cytosolic phospholipase A2 beta antibody
- DKFZp686N12131 antibody
Anti-cPLA2 beta antibody (ab53421) at 1/1000 dilution (in 1% Marvel + TBST for 1 hour at 22°C ) + Whole cell lysate of Human A549 cells at 10 µg
An HRP-conjugated donkey anti rabbit polyclonal at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 114 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
Exposure time: 8 minutes
Blocking Step: 5% Milk for 1 hour at 22°C
ab53421 has not yet been referenced specifically in any publications.