CPM, Thiol-reactive fluorescent probe (ab145275)

Overview

  • Product name

    CPM, Thiol-reactive fluorescent probe
  • Description

    Thiol-reactive fluorescent probe.
  • Alternative names

    • 7-Diethylamino-3-(4-maleimidylphenyl)-4-methylcoumarin
    • N-(4-(7-diethylamino-4-methylcoumarin-3-yl) phenyl) maleimide
  • Biological description

    Widely used blue fluorescent thiol-reactive dye. Essentially non-fluorescent until it reacts with thiols, making it possible to quantify thiols without a separation step. Good energy acceptor from tryptophan and a good energy donor to fluorescein. Used to monitor release of thiols and to distinguish proliferating cancer cells by nucleolar protein staining.

  • Purity

    > 95%
  • CAS Number

    76877-33-3
  • Chemical structure

    Chemical Structure

Properties

  • Excitation

    391nm
  • Emission

    472nm
  • Chemical name

    7-Diethylamino-3-(4-maleimidylphenyl)-4-methylcoumarin
  • Molecular weight

    402.44
  • Molecular formula

    C24H22N2O4
  • Storage instructions

    Store at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months.
  • Solubility overview

    Soluble in DMSO
  • Handling

    Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

    Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.

  • Source

    Synthetic

References

This product has been referenced in:

  • Chung CC  et al. A fluorescence-based thiol quantification assay for ultra-high-throughput screening for inhibitors of coenzyme A production. Assay Drug Dev Technol 6:361-74 (2008). Read more (PubMed: 18452391) »
  • Feng W  et al. Site-selective modification of hyperreactive cysteines of ryanodine receptor complex by quinones. Mol Pharmacol 55:821-31 (1999). Read more (PubMed: 10220560) »
  • Liu G & Pessah IN Molecular interaction between ryanodine receptor and glycoprotein triadin involves redox cycling of functionally important hyperreactive sulfhydryls. J Biol Chem 269:33028-34 (1994). Read more (PubMed: 7806531) »
  • Liu G  et al. Direct evidence for the existence and functional role of hyperreactive sulfhydryls on the ryanodine receptor-triadin complex selectively labeled by the coumarin maleimide 7-diethylamino-3-(4'-maleimidylphenyl)-4-methylcoumarin. Mol Pharmacol 45:189-200 (1994). Read more (PubMed: 8114670) »
  • Sippel TO Microfluorometric analysis of protein thiol groups with a coumarinylphenylmaleimide. J Histochem Cytochem 29:1377-81 (1981). Read more (PubMed: 7320496) »

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES, NOT FOR USE IN HUMANS"
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