Key features and details
- Thiol-reactive fluorescent probe.
- CAS Number: 76877-33-3
- Purity: > 95%
- Soluble in DMSO
- Form / State: Solid
- Source: Synthetic
Product nameCPM, Thiol-reactive fluorescent probe
DescriptionThiol-reactive fluorescent probe.
- N-(4-(7-diethylamino-4-methylcoumarin-3-yl) phenyl) maleimide
Widely used blue fluorescent thiol-reactive dye. Essentially non-fluorescent until it reacts with thiols, making it possible to quantify thiols without a separation step. Good energy acceptor from tryptophan and a good energy donor to fluorescein. Used to monitor release of thiols and to distinguish proliferating cancer cells by nucleolar protein staining.
Storage instructionsStore at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months.
Solubility overviewSoluble in DMSO
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab145275 has not yet been referenced specifically in any publications.