Recombinant Anti-CPN1 antibody [EPR6229] (ab124874)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6229] to CPN1
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-CPN1 antibody [EPR6229]
See all CPN1 primary antibodies -
Description
Rabbit monoclonal [EPR6229] to CPN1 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,IHC-P or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human CPN1 aa 100-200. The exact sequence is proprietary.
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Positive control
- Human plasma, HepG2 and Human fetal liver lysates
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR6229 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab124874 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Detects a band of approximately 55, 48, 27 kDa (predicted molecular weight: 52 kDa).
Note: The 48-kDa form is generated by proteolytic cleavage at the C-terminus. This subunitis cleaved into a peptide |
Notes |
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WB
1/1000 - 1/10000. Detects a band of approximately 55, 48, 27 kDa (predicted molecular weight: 52 kDa). Note: The 48-kDa form is generated by proteolytic cleavage at the C-terminus. This subunitis cleaved into a peptide |
Target
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Function
Protects the body from potent vasoactive and inflammatory peptides containing C-terminal Arg or Lys (such as kinins or anaphylatoxins) which are released into the circulation. -
Tissue specificity
Synthesized in the liver and secreted in plasma. -
Involvement in disease
Defects in CPN1 are the cause of carboxypeptidase N deficiency (CPND) [MIM:212070]. Patients affected present some combination of angioedema or chronic urticaria, as well as hay fever or astma, and have also slightly depressed serum carboxy peptidase N, suggestive of autosomal recessive inheritance of this disorder. -
Sequence similarities
Belongs to the peptidase M14 family. -
Cellular localization
Secreted > extracellular space. - Information by UniProt
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Database links
- Entrez Gene: 1369 Human
- Omim: 603103 Human
- SwissProt: P15169 Human
- Unigene: 2246 Human
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Alternative names
- ACBP antibody
- Anaphylatoxin inactivator antibody
- Arginine carboxypeptidase antibody
see all
Images
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All lanes : Anti-CPN1 antibody [EPR6229] (ab124874) at 1/1000 dilution
Lane 1 : Human plasma lysate
Lane 2 : HepG2 lysate
Lane 3 : Human fetal liver lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit
at 1/2000 dilution
Predicted band size: 52 kDa
Additional bands at: 27 kDa (possible cleavage fragment), 48 kDa (possible cleavage fragment)
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab124874 has not yet been referenced specifically in any publications.