Overview

  • Product name

    Anti-CPN1 antibody [EPR6229]
    See all CPN1 primary antibodies
  • Description

    Rabbit monoclonal [EPR6229] to CPN1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
    Unsuitable for: Flow Cyt,IHC-P or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human CPN1 aa 100-200. The exact sequence is proprietary.

  • Positive control

    • Human plasma, HepG2 and Human fetal liver lysates
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR6229
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab124874 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 55, 48, 27 kDa (predicted molecular weight: 52 kDa). Note: The 48-kDa form is generated by proteolytic cleavage at the C-terminus. This subunit is cleaved into a peptide
ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt,IHC-P or IP.
  • Target

    • Function

      Protects the body from potent vasoactive and inflammatory peptides containing C-terminal Arg or Lys (such as kinins or anaphylatoxins) which are released into the circulation.
    • Tissue specificity

      Synthesized in the liver and secreted in plasma.
    • Involvement in disease

      Defects in CPN1 are the cause of carboxypeptidase N deficiency (CPND) [MIM:212070]. Patients affected present some combination of angioedema or chronic urticaria, as well as hay fever or astma, and have also slightly depressed serum carboxy peptidase N, suggestive of autosomal recessive inheritance of this disorder.
    • Sequence similarities

      Belongs to the peptidase M14 family.
    • Cellular localization

      Secreted > extracellular space.
    • Information by UniProt
    • Database links

    • Alternative names

      • ACBP antibody
      • Anaphylatoxin inactivator antibody
      • Arginine carboxypeptidase antibody
      • Carboxypeptidase N catalytic chain antibody
      • Carboxypeptidase N polypeptide 1 50 kD antibody
      • Carboxypeptidase N polypeptide 1 antibody
      • Carboxypeptidase N small subunit antibody
      • Carboxypeptidase N subunit 1 antibody
      • CBPN_HUMAN antibody
      • CPN antibody
      • CPN1 antibody
      • Kininase 1 antibody
      • Kininase-1 antibody
      • Kininase1 antibody
      • Lysine carboxypeptidase antibody
      • Plasma carboxypeptidase B antibody
      • SCPN antibody
      • Serum carboxypeptidase N antibody
      see all

    Images

    • All lanes : Anti-CPN1 antibody [EPR6229] (ab124874) at 1/1000 dilution

      Lane 1 : Human plasma lysate
      Lane 2 : HepG2 lysate
      Lane 3 : Human fetal liver lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit
      at 1/2000 dilution

      Predicted band size: 52 kDa
      Additional bands at: 27 kDa (possible cleavage fragment), 48 kDa (possible cleavage fragment)

    References

    ab124874 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab124874.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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