Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6229] to CPN1
- Suitable for: WB, ICC/IF
- Reacts with: Human
Product nameAnti-CPN1 antibody [EPR6229]
See all CPN1 primary antibodies
DescriptionRabbit monoclonal [EPR6229] to CPN1
Tested applicationsSuitable for: WB, ICC/IFmore details
Unsuitable for: Flow Cyt,IHC-P or IP
Species reactivityReacts with: Human
Synthetic peptide within Human CPN1 aa 100-200. The exact sequence is proprietary.
- Human plasma, HepG2 and Human fetal liver lysates
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab124874 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 55, 48, 27 kDa (predicted molecular weight: 52 kDa). Note: The 48-kDa form is generated by proteolytic cleavage at the C-terminus. This subunit is cleaved into a peptide|
|ICC/IF||1/100 - 1/250.|
FunctionProtects the body from potent vasoactive and inflammatory peptides containing C-terminal Arg or Lys (such as kinins or anaphylatoxins) which are released into the circulation.
Tissue specificitySynthesized in the liver and secreted in plasma.
Involvement in diseaseDefects in CPN1 are the cause of carboxypeptidase N deficiency (CPND) [MIM:212070]. Patients affected present some combination of angioedema or chronic urticaria, as well as hay fever or astma, and have also slightly depressed serum carboxy peptidase N, suggestive of autosomal recessive inheritance of this disorder.
Sequence similaritiesBelongs to the peptidase M14 family.
Cellular localizationSecreted > extracellular space.
- Information by UniProt
- ACBP antibody
- Anaphylatoxin inactivator antibody
- Arginine carboxypeptidase antibody
All lanes : Anti-CPN1 antibody [EPR6229] (ab124874) at 1/1000 dilution
Lane 1 : Human plasma lysate
Lane 2 : HepG2 lysate
Lane 3 : Human fetal liver lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit
at 1/2000 dilution
Predicted band size: 52 kDa
Additional bands at: 27 kDa (possible cleavage fragment), 48 kDa (possible cleavage fragment)
ab124874 has not yet been referenced specifically in any publications.