Recombinant Anti-EPF antibody [EPR4476] (ab108600)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4476] to EPF
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
Related conjugates and formulations
Overview
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Product name
Anti-EPF antibody [EPR4476]
See all EPF primary antibodies -
Description
Rabbit monoclonal [EPR4476] to EPF -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details
Unsuitable for: Flow Cyt -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: A431, Neuro-2a, A549, and MCF7 cell lysates IHC-P: Human colon and kidney tissues ICC/IF: HeLa cells
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR4476 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab108600 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | (4) |
1/10000 - 1/50000. Detects a band of approximately 11 kDa (predicted molecular weight: 11 kDa).
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IHC-P |
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
1/100 - 1/250.
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Notes |
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WB
1/10000 - 1/50000. Detects a band of approximately 11 kDa (predicted molecular weight: 11 kDa). |
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
1/100 - 1/250. |
Target
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Function
Eukaryotic CPN10 homolog which is essential for mitochondrial protein biogenesis, together with CPN60. Binds to CPN60 in the presence of Mg-ATP and suppresses the ATPase activity of the latter. -
Sequence similarities
Belongs to the GroES chaperonin family. -
Cellular localization
Mitochondrion matrix. - Information by UniProt
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Database links
- Entrez Gene: 3336 Human
- Entrez Gene: 15528 Mouse
- Entrez Gene: 25462 Rat
- Omim: 600141 Human
- SwissProt: P61604 Human
- SwissProt: Q64433 Mouse
- SwissProt: P26772 Rat
- Unigene: 1197 Human
see all -
Alternative names
- 10 kDa chaperonin antibody
- 10 kDa heat shock protein mitochondrial antibody
- 10 kDa heat shock protein, mitochondrial antibody
see all
Images
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All lanes : Anti-EPF antibody [EPR4476] (ab108600) at 1/10000 dilution
Lane 1 : A431 cell lysate
Lane 2 : Neuro-2a cell lysate
Lane 3 : A549 cell lysate
Lane 4 : MCF7 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 11 kDa
Observed band size: 11 kDa -
Immunohistochemical staining of EPF in paraffin embedded Human colon tissue, using ab108600 at a dilution of 1/250.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemical staining of EPF in paraffin embedded Human kidney tissue, using ab108600 at a dilution of 1/250.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunofluorescent staining of EPF in HeLa cells, using ab108600 at a dilution of 1/100.
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ab108600 showing positive staining in Normal uterus tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ab108600 showing positive staining in Ovarian carcinoma tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ab108600 showing positive staining in Normal breast tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ab108600 showing positive staining in Colonic adenocarcinoma tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ab108600 showing positive staining in Normal stomach tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (7)
ab108600 has been referenced in 7 publications.
- Mati Q et al. Tissue Nuclear Matrix Protein Expression 22 in Various Grades and Stages of Bladder Cancer. J Coll Physicians Surg Pak 30:1321-1325 (2020). PubMed: 33397061
- Wyzewski Z et al. Mitochondrial Heat Shock Response Induced by Ectromelia Virus is Accompanied by Reduced Apoptotic Potential in Murine L929 Fibroblasts. Arch Immunol Ther Exp (Warsz) N/A:N/A (2019). PubMed: 31324924
- Song H et al. Global profiling of O-GlcNAcylated and/or phosphorylated proteins in hepatoblastoma. Signal Transduct Target Ther 4:40 (2019). PubMed: 31637018
- Druck T et al. Fhit-Fdxr interaction in the mitochondria: modulation of reactive oxygen species generation and apoptosis in cancer cells. Cell Death Dis 10:147 (2019). PubMed: 30770797
- Tsai CH et al. Systematic verification of bladder cancer-associated tissue protein biomarker candidates in clinical urine specimens. Oncotarget 9:30731-30747 (2018). PubMed: 30112103
- Sakellariou GK et al. Neuron-specific expression of CuZnSOD prevents the loss of muscle mass and function that occurs in homozygous CuZnSOD-knockout mice. FASEB J 28:1666-81 (2014). WB ; Mouse . PubMed: 24378874
- Gispert S et al. Loss of mitochondrial peptidase Clpp leads to infertility, hearing loss plus growth retardation via accumulation of CLPX, mtDNA and inflammatory factors. Hum Mol Genet N/A:N/A (2013). PubMed: 23851121