Overview

  • Product name
  • Description
    Rabbit polyclonal to CPNE1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rabbit, Cow, Dog, Pig
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 250-537 of Human CPNE1 (UniProt ID: Q99829).

  • Positive control
    • 293T, A431, H1299, HeLaS3, HepG2, MOLT4, Raji and Mouse brain whole cell lysates; Human Cal27 xenograft tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 0.75% Glycine, 1.21% Tris, 10% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab155675 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 59 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

Images

  • All lanes : Anti-CPNE1 antibody (ab155675) at 1/1000 dilution

    Lane 1 : 293T whole cell lysate
    Lane 2 : A431 whole cell lysate
    Lane 3 : H1299 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 59 kDa



    7.5% SDS PAGE
  • Anti-CPNE1 antibody (ab155675) at 1/1000 dilution + Mouse brain whole cell lysate at 50 µg

    Predicted band size: 59 kDa



    7.5% SDS PAGE
  • Immunohistochemical analysis of paraffin-embedded Human Cal27 xenograft tissue labeling CPNE1 with ab155675 at 1/100 dilution.

References

This product has been referenced in:
  • Tang H  et al. CPNE1 is a target of miR-335-5p and plays an important role in the pathogenesis of non-small cell lung cancer. J Exp Clin Cancer Res 37:131 (2018). Read more (PubMed: 29970127) »
See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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