Product nameAnti-CPNE1 antibody
See all CPNE1 primary antibodies
DescriptionRabbit polyclonal to CPNE1
Tested applicationsSuitable for: WB, ELISA, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Sheep, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig
- HepG2 cell lysate. Human intestine.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 2% Sucrose, PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab66898 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 5 µg/ml. Detects a band of approximately 57 kDa (predicted molecular weight: 59 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
|ELISA||Use at an assay dependent concentration.
Titre using peptide based assay: 1/312500.
|IHC-P||Use a concentration of 4 - 8 µg/ml.|
FunctionMay function in membrane trafficking. Exhibits calcium-dependent phospholipid binding properties.
Sequence similaritiesBelongs to the copine family.
Contains 2 C2 domains.
Contains 1 VWFA domain.
- Information by UniProt
- Copine 1 antibody
- Copine I antibody
- Copine-1 antibody
All lanes : Anti-CPNE1 antibody (ab66898) at 5 µg/ml (in 5% skim milk / PBS buffer)
Lane 1 : Molecular weight markers
Lane 2 : HepG2 cell lysate at 10 µg
All lanes : HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size: 59 kDa
Observed band size: 57 kDa why is the actual band size different from the predicted?
Additional bands at: 25 kDa. We are unsure as to the identity of these extra bands.
Gel concentration 12%.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human intestine tissue labelling CPNE1 with ab66898 at 4-8µg/ml. Arrows indicate positively labelled epithelial cells of the intestinal villus. Magnification: 400X.
ab66898 has not yet been referenced specifically in any publications.