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    cpox-antibody-ab236613.pdf

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Signal Transduction Metabolism Mitochondrial
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Anti-CPOX antibody (ab236613)

  • Datasheet
  • SDS
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPOX antibody (ab236613)
  • Western blot - Anti-CPOX antibody (ab236613)
  • Immunocytochemistry/ Immunofluorescence - Anti-CPOX antibody (ab236613)

Key features and details

  • Rabbit polyclonal to CPOX
  • Suitable for: ICC/IF, IHC-P, WB
  • Reacts with: Human
  • Isotype: IgG

You may also be interested in

Protein
Product image
Recombinant Human CPOX protein (ab156972)
Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-CPOX antibody
    See all CPOX primary antibodies
  • Description

    Rabbit polyclonal to CPOX
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human CPOX aa 19-149.
    Sequence:

    GGCGGPRAWSQCGGGGLRAWSQRSAAGRVCRPPGPAGTEQSRGLGHGSTS RGGPWVGTGLAAALAGLVGLATAAFGHVQRAEMLPKTSGTRATSLGRPEE EEDELAHRCSSFMAPPVTDLGELRRRPGDMK


    Database link: P36551
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • WB: HepG2 and A549 whole cell lysates. IHC-P: Human liver tissue. ICC/IF: HepG2 cells.
  • General notes

    Previously labelled as Coproporphyrinogen Oxidase (CPOX).

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.03% Proclin 300
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    Purity >95%.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Metabolism
    • Mitochondrial
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Mitochondrial markers

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • HepG2 whole cell lysate (ab166833)
    • A549 whole cell lysate (ab7910)
  • Recombinant Protein

    • Recombinant Human CPOX protein (ab156972)

Applications

Our Abpromise guarantee covers the use of ab236613 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/50 - 1/200.
IHC-P 1/200 - 1/500.
WB 1/500 - 1/5000. Predicted molecular weight: 50 kDa.

Target

  • Function

    Key enzyme in heme biosynthesis. Catalyzes the oxidative decarboxylation of propionic acid side chains of rings A and B of coproporphyrinogen III.
  • Pathway

    Porphyrin metabolism; protoporphyrin-IX biosynthesis; protoporphyrinogen-IX from coproporphyrinogen-III (O2 route): step 1/1.
  • Involvement in disease

    Defects in CPOX are the cause of hereditary coproporphyria (HCP) [MIM:121300]. HCP is an acute hepatic porphyria and an autosomal dominant disease characterized by neuropsychiatric disturbances and skin photosensitivity. Biochemically, there is an overexcretion of coproporphyrin III in the urine and in the feces. HCP is clinically characterized by attacks of abdominal pain, neurological disturbances, and psychiatric symptoms. The symptoms are generally manifested with rapid onset, and can be precipitated by drugs, alcohol, caloric deprivation, infection, endocrine factors or stress. A severe variant form is harderoporphyria, which is characterized by earlier onset attacks, massive excretion of harderoporphyrin in the feces, and a marked decrease of coproporphyrinogen IX oxidase activity.
  • Sequence similarities

    Belongs to the aerobic coproporphyrinogen-III oxidase family.
  • Cellular localization

    Mitochondrion intermembrane space.
  • Target information above from: UniProt accession P36551 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 1371 Human
    • Omim: 612732 Human
    • SwissProt: P36551 Human
    • Unigene: 476982 Human
    • Alternative names

      • Coprogen oxidase antibody
      • COPROPORPHYRIA antibody
      • Coproporphyrinogen III oxidase antibody
      • Coproporphyrinogen Oxidase (CPOX) antibody
      • Coproporphyrinogen-III oxidase antibody
      • Coproporphyrinogenase antibody
      • COX antibody
      • CPO antibody
      • Cpox antibody
      • CPX antibody
      • HEM 6 antibody
      • Hem-6 antibody
      • Hem6 antibody
      • HEM6_HUMAN antibody
      • mitochondrial antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPOX antibody (ab236613)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPOX antibody (ab236613)

      Paraffin-embedded human liver tissue stained for CPOX using ab236613 at 1/300 dilution in immunohistochemical analysis.

      After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

    • Western blot - Anti-CPOX antibody (ab236613)
      Western blot - Anti-CPOX antibody (ab236613)
      All lanes : Anti-CPOX antibody (ab236613) at 1/500 dilution

      Lane 1 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
      Lane 2 : A549 (Human lung carcinoma cell line) whole cell lysate

      Secondary
      All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution

      Predicted band size: 50 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-CPOX antibody (ab236613)
      Immunocytochemistry/ Immunofluorescence - Anti-CPOX antibody (ab236613)

      HepG2 (Human liver hepatocellular carcinoma cell line) cells stained for CPOX (green) using ab236613 at 1/100 dilution in ICC/IF. Secondary antibody is an Alexa-Fluor® 488-conjugated Goat Anti-Rabbit IgG (H+L).

      The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal goat serum. The cells were then incubated with the antibody overnight at 4°C.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (0)

    Publishing research using ab236613? Please let us know so that we can cite the reference in this datasheet.

    ab236613 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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