Recombinant
RabMAb

Recombinant Anti-CPOX antibody [EPR11926(B)] (ab170945)

Overview

  • Product name

    Anti-CPOX antibody [EPR11926(B)]
    See all CPOX primary antibodies
  • Description

    Rabbit monoclonal [EPR11926(B)] to CPOX
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, Flow Cytmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity

    Reacts with: Human
    Does not react with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Human CPOX aa 100-200 (Cysteine residue). The exact sequence is proprietary.
    Database link: P36551

  • Positive control

    • HepG2, K562, LnCaP, and Jurkat cell lysates; HepG2 and K562 cells.
  • General notes

    Previously labelled as Coproporphyrinogen Oxidase (CPOX).

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab170945 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Predicted molecular weight: 50 kDa.
ICC/IF 1/100 - 1/200.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for IHC-P or IP.
  • Target

    • Function

      Key enzyme in heme biosynthesis. Catalyzes the oxidative decarboxylation of propionic acid side chains of rings A and B of coproporphyrinogen III.
    • Pathway

      Porphyrin metabolism; protoporphyrin-IX biosynthesis; protoporphyrinogen-IX from coproporphyrinogen-III (O2 route): step 1/1.
    • Involvement in disease

      Defects in CPOX are the cause of hereditary coproporphyria (HCP) [MIM:121300]. HCP is an acute hepatic porphyria and an autosomal dominant disease characterized by neuropsychiatric disturbances and skin photosensitivity. Biochemically, there is an overexcretion of coproporphyrin III in the urine and in the feces. HCP is clinically characterized by attacks of abdominal pain, neurological disturbances, and psychiatric symptoms. The symptoms are generally manifested with rapid onset, and can be precipitated by drugs, alcohol, caloric deprivation, infection, endocrine factors or stress. A severe variant form is harderoporphyria, which is characterized by earlier onset attacks, massive excretion of harderoporphyrin in the feces, and a marked decrease of coproporphyrinogen IX oxidase activity.
    • Sequence similarities

      Belongs to the aerobic coproporphyrinogen-III oxidase family.
    • Cellular localization

      Mitochondrion intermembrane space.
    • Information by UniProt
    • Database links

    • Alternative names

      • Coprogen oxidase antibody
      • COPROPORPHYRIA antibody
      • Coproporphyrinogen III oxidase antibody
      • Coproporphyrinogen Oxidase (CPOX) antibody
      • Coproporphyrinogen-III oxidase antibody
      • Coproporphyrinogenase antibody
      • COX antibody
      • CPO antibody
      • Cpox antibody
      • CPX antibody
      • HEM 6 antibody
      • Hem-6 antibody
      • Hem6 antibody
      • HEM6_HUMAN antibody
      • mitochondrial antibody
      see all

    Images

    • All lanes : Anti-CPOX antibody [EPR11926(B)] (ab170945) at 1/10000 dilution

      Lane 1 : HepG2 cell lysate
      Lane 2 : K562 cell lysate
      Lane 3 : LnCaP cell lysate
      Lane 4 : Jurkat cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 50 kDa

    • Immunofluorescence analysis of HepG2 cells labeling CPOX with ab170945 at 1/100 (red). DAPI nuclear staining (blue).

    • Flow cytometric analysis of K562 cells labeling CPOX with ab170945 at 1/10 dilution (red) and rabbit IgG (negative control) (green).

    References

    ab170945 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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