Overview

  • Product name

    Anti-CPOX antibody [EPR11927(B)]
    See all CPOX primary antibodies
  • Description

    Rabbit monoclonal [EPR11927(B)] to CPOX
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, IP, Flow Cytmore details
    Unsuitable for: IHC-P
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human CPOX (Cysteine residue). The exact sequence is proprietary.
    Database link: P36551

  • Positive control

    • K562, HCT-116, Jurkat, LnCaP, fetal liver and HepG2 lysates, Jurkat cells, K562 cells
  • General notes

     

     

    Previously labelled as Coproporphyrinogen Oxidase (CPOX).

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab169766 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 36 kDa (predicted molecular weight: 50 kDa).
ICC/IF 1/50 - 1/100.
IP 1/10 - 1/100.
Flow Cyt 1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for IHC-P.
  • Target

    • Function

      Key enzyme in heme biosynthesis. Catalyzes the oxidative decarboxylation of propionic acid side chains of rings A and B of coproporphyrinogen III.
    • Pathway

      Porphyrin metabolism; protoporphyrin-IX biosynthesis; protoporphyrinogen-IX from coproporphyrinogen-III (O2 route): step 1/1.
    • Involvement in disease

      Defects in CPOX are the cause of hereditary coproporphyria (HCP) [MIM:121300]. HCP is an acute hepatic porphyria and an autosomal dominant disease characterized by neuropsychiatric disturbances and skin photosensitivity. Biochemically, there is an overexcretion of coproporphyrin III in the urine and in the feces. HCP is clinically characterized by attacks of abdominal pain, neurological disturbances, and psychiatric symptoms. The symptoms are generally manifested with rapid onset, and can be precipitated by drugs, alcohol, caloric deprivation, infection, endocrine factors or stress. A severe variant form is harderoporphyria, which is characterized by earlier onset attacks, massive excretion of harderoporphyrin in the feces, and a marked decrease of coproporphyrinogen IX oxidase activity.
    • Sequence similarities

      Belongs to the aerobic coproporphyrinogen-III oxidase family.
    • Cellular localization

      Mitochondrion intermembrane space.
    • Information by UniProt
    • Database links

    • Alternative names

      • Coprogen oxidase antibody
      • COPROPORPHYRIA antibody
      • Coproporphyrinogen III oxidase antibody
      • Coproporphyrinogen Oxidase (CPOX) antibody
      • Coproporphyrinogen-III oxidase antibody
      • Coproporphyrinogenase antibody
      • COX antibody
      • CPO antibody
      • Cpox antibody
      • CPX antibody
      • HEM 6 antibody
      • Hem-6 antibody
      • Hem6 antibody
      • HEM6_HUMAN antibody
      • mitochondrial antibody
      see all

    Images

    • Detection of CPOX
      by Western Blot of Immunprecipitate.
      Human fetal liver lysate (lane 1)  or 1 X PBS (lane 2) immunoprecipitated using ab169766 at 1/10 dilution.



      All lanes : Anti-CPOX antibody [EPR11927(B)] (ab169766) at 1/1000 dilution

      Lane 1 : Anti-CPOX antibody [EPR11927(B)] (ab169766) at 1/10 dilution
      Lane 2 : negative control
    • Flow Cytometric analysis of permeabilized K562 cells labeling CPOX with ab169766 at 1/100 dilution (red) or a rabbit IgG (negative) (green).

    • Immunofluorescent analysis of Jurkat cells labeling CPOX with ab169766 at 1/50 dilution.

    • All lanes : Anti-CPOX antibody [EPR11927(B)] (ab169766) at 1/1000 dilution

      Lane 1 : K562 lysate
      Lane 2 : HCT-116 lysate
      Lane 3 : Jurkat lysate
      Lane 4 : LnCaP lysate
      Lane 5 : Human fetal liver lysate
      Lane 6 : HepG2 lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 50 kDa

    References

    This product has been referenced in:

    • Maio N  et al. Dimeric ferrochelatase bridges ABCB7 and ABCB10 homodimers in an architecturally defined molecular complex required for heme biosynthesis. Haematologica N/A:N/A (2019). Read more (PubMed: 30765471) »
    See 1 Publication for this product

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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