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    cps1-antibody-ab3682.pdf

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Anti-CPS1 antibody (ab3682)

  • Datasheet
  • SDS
Reviews (9)Q&A (3)References (19)

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Western blot - Anti-CPS1 antibody (ab3682)
  • Immunocytochemistry/ Immunofluorescence - Anti-CPS1 antibody (ab3682)
  • Western blot - Anti-CPS1 antibody (ab3682)

Key features and details

  • Rabbit polyclonal to CPS1
  • Suitable for: ICC/IF, WB
  • Reacts with: African green monkey
  • Isotype: IgG

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Overview

  • Product name

    Anti-CPS1 antibody
    See all CPS1 primary antibodies
  • Description

    Rabbit polyclonal to CPS1
  • Host species

    Rabbit
  • Specificity

    When the antibody is used at 1/2000 some lower molecular weight bands appear in COS-7 cells, the extent of which is significantly reduced when the antibody is used at 1/10,000.
  • Tested Applications & Species

    Application Species
    ICC/IF
    African green monkey
    WB
    African green monkey
    See all applications and species data
  • Immunogen

    Full length native protein (purified) (Rat).

  • General notes

    OMIM reference: 114010 Alternative name: carbamoylphosphate synthetase 1, CAD

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Add glycerol to a final volume of 50% for extra stability and aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.02% Sodium azide
  • Concentration information loading...
  • Purity

    Whole antiserum
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Tags & Cell Markers
    • Subcellular Markers
    • Organelles
    • Mitochondria
    • Signal Transduction
    • Metabolism
    • Amino Acids
    • Signal Transduction
    • Metabolism
    • Mitochondrial
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Mitochondrial markers
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Amino acid metabolism
    • Metabolism
    • Types of disease
    • Cancer

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Mouse monoclonal [SB62a] Anti-Rabbit IgG light chain (HRP) (ab99697)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human CPS1 protein (ab152296)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab3682 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC/IF
African green monkey
WB
African green monkey
Application Abreviews Notes
ICC/IF (1)
Use at an assay dependent concentration.
WB (5)
Use at an assay dependent concentration.
Notes
ICC/IF
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration.

Target

  • Function

    Involved in the urea cycle of ureotelic animals where the enzyme plays an important role in removing excess ammonia from the cell.
  • Tissue specificity

    Primarily in the liver and small intestine.
  • Involvement in disease

    Defects in CPS1 are the cause of carbamoyl phosphate synthetase 1 deficiency (CPS1D) [MIM:237300]. CPS1D is an autosomal recessive disorder of the urea cycle causing hyperammonemia. Clinical features include protein intolerance, intermittent ataxia, seizures, lethargy, developmental delay and mental retardation.
    Note=Genetic variations in CPS1 influence the availability of precursors for nitric oxide (NO) synthesis and play a role in clinical situations where endogenous NO production is critically important, such as neonatal pulmonary hypertension, increased pulmonary artery pressure following surgical repair of congenital heart defects or hepatovenocclusive disease following bone marrow transplantation. Infants with neonatal pulmonary hypertension homozygous for Thr-1406 have lower L-arginine concentrations than neonates homozygous for Asn-1406.
  • Sequence similarities

    Contains 2 ATP-grasp domains.
    Contains 1 glutamine amidotransferase type-1 domain.
  • Domain

    The type-1 glutamine amidotransferase domain is defective.
  • Cellular localization

    Mitochondrion.
  • Target information above from: UniProt accession P31327 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Alternative names

    • Carbamoyl phosphate synthase [ammonia] antibody
    • Carbamoyl phosphate synthase [ammonia] mitochondrial antibody
    • Carbamoyl phosphate synthase antibody
    • Carbamoyl phosphate synthetase 1 antibody
    • Carbamoyl phosphate synthetase 1 mitochondrial antibody
    • Carbamoyl phosphate synthetase I antibody
    • Carbamoyl-phosphate synthase [ammonia] antibody
    • Carbamoyl-phosphate synthetase I antibody
    • Carbamoylphosphate synthase antibody
    • Carbamoylphosphate synthetase 1 antibody
    • Carbamoylphosphate synthetase I antibody
    • CPS 1 antibody
    • Cps1 antibody
    • CPSase 1 antibody
    • CPSase I antibody
    • CPSASE1 antibody
    • CPSM_HUMAN antibody
    • mitochondrial antibody
    • MS738 antibody
    see all

Images

  • Western blot - Anti-CPS1 antibody (ab3682)
    Western blot - Anti-CPS1 antibody (ab3682)

    Lane 1: 5 ul of 2ml lysate cos-7 cells transfected with full length CPS 1. Before IP
    Lane 2: cos-7 cells transfected with full length CPS 1. IP with 5 ul of pre-immune serum
    Lane 3: cos-7 cells transfected with full length CPS 1. IP with 2 ul of polyclonal antibody anti CPS 1 (ab3682)
    Lane 4: cos-7 cells transfected with full length CPS 1. IP with 5 ul of polyclonal antibody anti CPS 1 (ab3682)
    Lane 5: 5 ul of 2ml lysate cos-7 cells transfected with empty vector (pCMV5-tr). Before IP
    Lane 6: cos-7 cells transfected with empty vector (pCMV5-tr). IP with 5 ul of polyclonal antibody anti CPS 1 (ab3682)
    Lane 7: 5 ul of 2ml lysate untransfected cos-7 cells. Before IP
    Lane 8: untransfected cos-7 cells. IP with 5 ul of polyclonal antibody anti CPS 1 (ab3682) Ab3682 can IP CPS1 transiently expressed in COS-7 cells.

    Lane 1: 5 ul of 2ml lysate cos-7 cells transfected with full length CPS 1. Before IP
    Lane 2: cos-7 cells transfected with full length CPS 1. IP

  • Immunocytochemistry/ Immunofluorescence - Anti-CPS1 antibody (ab3682)
    Immunocytochemistry/ Immunofluorescence - Anti-CPS1 antibody (ab3682)

    CPS-1 localisation is consistent with the reticulated pattern of liver mitonchondria.

    Rabbit polyclonal to CPS-1 (ab3682), at 1/5000 on COS-7 (African green monkey kidney fibroblast-like cell line) cells transiently transfected with pCMV5-CPS 1 plasmid (Corvi, M.M and Berthiaume, L.G. unpublished data).

    Secondary: donkey anti-rabbit (Texas Red).

  • Western blot - Anti-CPS1 antibody (ab3682)
    Western blot - Anti-CPS1 antibody (ab3682)

    Western blot using Rabbit polyclonal to CPS 1 (ab3682). All lanes loaded with 100 ng of purified CPS 1
    Lane 1: ab3682 1/500
    Lane 2: ab3682 1/1000
    Lane 3: ab3682 1/2000 Western blot using Rabbit polyclonal to CPS 1 (ab3682).

    All lanes loaded with 100 ng of purified CPS 1

    Lane 1: ab3682 1/500
    Lane 2: ab3682 1/1000
    Lane 3: ab3682 1/2000

Protocols

  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (19)

Publishing research using ab3682? Please let us know so that we can cite the reference in this datasheet.

ab3682 has been referenced in 19 publications.

  • Alsaeedi F  et al. Expression of serine/threonine protein kinase SGK1F promotes an hepatoblast state in stem cells directed to differentiate into hepatocytes. PLoS One 14:e0218135 (2019). PubMed: 31242206
  • Tata PR  et al. Developmental History Provides a Roadmap for the Emergence of Tumor Plasticity. Dev Cell 44:679-693.e5 (2018). PubMed: 29587142
  • Wang T  et al. L-leucine stimulates glutamate dehydrogenase activity and glutamate synthesis by regulating mTORC1/SIRT4 pathway in pig liver. Anim Nutr 4:329-337 (2018). PubMed: 30175263
  • Peng WC  et al. Inflammatory Cytokine TNFa Promotes the Long-Term Expansion of Primary Hepatocytes in 3D Culture. Cell 175:1607-1619.e15 (2018). PubMed: 30500539
  • Kang YBA  et al. Metabolic Patterning on a Chip: Towards in vitro Liver Zonation of Primary Rat and Human Hepatocytes. Sci Rep 8:8951 (2018). ICC/IF . PubMed: 29895900
View all Publications for this product

Customer reviews and Q&As

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1-10 of 12 Abreviews or Q&A

Western blot abreview for Anti-CPS1 antibody - Liver Mitochondrial Marker

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Reduced Denaturing (12%)
Sample
Rat Tissue lysate - whole (Liver)
Specification
Liver
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Read More

Abcam user community

Verified customer

Submitted Nov 11 2013

Western blot abreview for Anti-CPS1 antibody - Liver Mitochondrial Marker

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Reduced Denaturing (12%)
Sample
Mouse Tissue lysate - whole (Liver)
Specification
Liver
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Read More

Abcam user community

Verified customer

Submitted Nov 07 2013

Immunocytochemistry/ Immunofluorescence abreview for Anti-CPS1 antibody - Liver Mitochondrial Marker

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Hepatocyte)
Specification
Hepatocyte
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 2%
Fixative
Formaldehyde
Read More

Abcam user community

Verified customer

Submitted Jun 12 2013

Western blot abreview for Anti-CPS1 antibody - Liver Mitochondrial Marker

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Rat Tissue lysate - whole (Liver)
Loading amount
50 µg
Specification
Liver
Gel Running Conditions
Reduced Denaturing (4-12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Read More

Abcam user community

Verified customer

Submitted Dec 25 2012

Western blot abreview for Anti-CPS1 antibody - Liver Mitochondrial Marker

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - whole (Liver)
Loading amount
50 µg
Specification
Liver
Gel Running Conditions
Reduced Denaturing (4-12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Read More

Abcam user community

Verified customer

Submitted Dec 25 2012

Question

Rezepte für Melate-Puffer

Read More

Abcam community

Verified customer

Asked on Aug 13 2012

Answer

Vielen Dank für Ihren Anruf heute Morgen.
Ich habe mir die Beschreibung des IHC-P Bildes nochmals angeschaut und muss ihnen leider mitteilen, dass ich Ihnen keine genauen Angaben über dem Maleate Puffer machen kann, da dieses Bild und die Beschreibung nicht aus unsrem Labor stammt. Eine Kundin hat einen Abreview als Feedback mi t diesem Bild eingeschickt.
https://www.abcam.com/CPS1-antibody-ab3682.html&tab=abreviews&intabreviewid=14931
Ich habe allerding Rezepte für Melate-Puffer gefunden:
http://www.nrims.harvard.edu/protocols/Uranyl_Acetate_Stain_in_Maleate_Buffer.pdf
http://www.ou.edu/research/electron/bmz5364/maleate.html
Ich hoffe, diese helfen Ihnen weiter und wünsche Ihnen viel Erfolg mit Ihren Experimenten.

Read More

Abcam Scientific Support

Answered on Aug 13 2012

Immunohistochemistry (Frozen sections) abreview for Anti-CPS1 antibody - Liver Mitochondrial Marker

Good
Abreviews
Abreviews
Application
Immunohistochemistry (Frozen sections)
Sample
Rat Tissue sections (Liver)
Specification
Liver
Fixative
Acetone
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
Read More

Abcam user community

Verified customer

Submitted Jul 06 2012

Western blot abreview for Anti-CPS1 antibody - Liver Mitochondrial Marker

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Rat Tissue lysate - whole (liver)
Loading amount
40 µg
Specification
liver
Treatment
17.5 mg/kg retrorsine for 24 h
Gel Running Conditions
Reduced Non-Denaturing (Native) (12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

Dr. Junyi Xue

Verified customer

Submitted Jul 03 2012

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CPS1 antibody - Liver Mitochondrial Marker

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (liver E18.5)
Specification
liver E18.5
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate buffer
Permeabilization
No
Blocking step
(agent) for 30 minute(s) · Concentration: 100% · Temperature: 22°C
Read More

Dr. Asha Seth

Verified customer

Submitted Jun 23 2009

Immunohistochemistry (Frozen sections) abreview for Anti-CPS1 antibody - Liver Mitochondrial Marker

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Liver PO)
Specification
Liver PO
Fixative
Paraformaldehyde
Permeabilization
No
Blocking step
maleate blocking solution as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
Read More

Dr. Asha Seth

Verified customer

Submitted Jun 23 2009

1-10 of 12 Abreviews or Q&A

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