Anti-CPS1 antibody (ab3682)
Key features and details
- Rabbit polyclonal to CPS1
- Suitable for: IHC-Fr, IHC-P, IP, ICC/IF, WB
- Reacts with: Mouse, Rat, African green monkey
- Isotype: IgG
Overview
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Product name
Anti-CPS1 antibody
See all CPS1 primary antibodies -
Description
Rabbit polyclonal to CPS1 -
Host species
Rabbit -
Specificity
When the antibody is used at 1/2000 some lower molecular weight bands appear in COS-7 cells, the extent of which is significantly reduced when the antibody is used at 1/10,000. -
Tested applications
Suitable for: IHC-Fr, IHC-P, IP, ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, African green monkey -
Immunogen
Full length native protein (purified) corresponding to Rat CPS1.
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General notes
OMIM reference: 114010 Alternative name: carbamoylphosphate synthetase 1, CADThe Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Add glycerol to a final volume of 50% for extra stability and aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Sodium azide -
Concentration information loading... -
Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab3682 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-Fr | (2) |
Use at an assay dependent concentration.
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| IHC-P | (1) |
Use at an assay dependent concentration.
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| IP |
Use at an assay dependent concentration.
|
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| ICC/IF | (1) |
Use at an assay dependent concentration.
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| WB | (5) |
Use at an assay dependent concentration.
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| Notes |
|---|
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IHC-Fr
Use at an assay dependent concentration. |
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IHC-P
Use at an assay dependent concentration. |
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IP
Use at an assay dependent concentration. |
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ICC/IF
Use at an assay dependent concentration. |
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WB
Use at an assay dependent concentration. |
Target
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Function
Involved in the urea cycle of ureotelic animals where the enzyme plays an important role in removing excess ammonia from the cell. -
Tissue specificity
Primarily in the liver and small intestine. -
Involvement in disease
Defects in CPS1 are the cause of carbamoyl phosphate synthetase 1 deficiency (CPS1D) [MIM:237300]. CPS1D is an autosomal recessive disorder of the urea cycle causing hyperammonemia. Clinical features include protein intolerance, intermittent ataxia, seizures, lethargy, developmental delay and mental retardation.
Note=Genetic variations in CPS1 influence the availability of precursors for nitric oxide (NO) synthesis and play a role in clinical situations where endogenous NO production is critically important, such as neonatal pulmonary hypertension, increased pulmonary artery pressure following surgical repair of congenital heart defects or hepatovenocclusive disease following bone marrow transplantation. Infants with neonatal pulmonary hypertension homozygous for Thr-1406 have lower L-arginine concentrations than neonates homozygous for Asn-1406. -
Sequence similarities
Contains 2 ATP-grasp domains.
Contains 1 glutamine amidotransferase type-1 domain. -
Domain
The type-1 glutamine amidotransferase domain is defective. -
Cellular localization
Mitochondrion. - Information by UniProt
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Database links
- Entrez Gene: 227231 Mouse
- Entrez Gene: 497840 Rat
- SwissProt: Q8C196 Mouse
- SwissProt: P07756 Rat
- Unigene: 343942 Mouse
- Unigene: 53968 Rat
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Alternative names
- Carbamoyl phosphate synthase [ammonia] antibody
- Carbamoyl phosphate synthase [ammonia] mitochondrial antibody
- Carbamoyl phosphate synthase antibody
see all
Images
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Western blot - Anti-CPS1 antibody (ab3682)
Lane 1: 5 ul of 2ml lysate cos-7 cells transfected with full length CPS 1. Before IP
Lane 2: cos-7 cells transfected with full length CPS 1. IP with 5 ul of pre-immune serum
Lane 3: cos-7 cells transfected with full length CPS 1. IP with 2 ul of polyclonal antibody anti CPS 1 (ab3682)
Lane 4: cos-7 cells transfected with full length CPS 1. IP with 5 ul of polyclonal antibody anti CPS 1 (ab3682)
Lane 5: 5 ul of 2ml lysate cos-7 cells transfected with empty vector (pCMV5-tr). Before IP
Lane 6: cos-7 cells transfected with empty vector (pCMV5-tr). IP with 5 ul of polyclonal antibody anti CPS 1 (ab3682)
Lane 7: 5 ul of 2ml lysate untransfected cos-7 cells. Before IP
Lane 8: untransfected cos-7 cells. IP with 5 ul of polyclonal antibody anti CPS 1 (ab3682) Ab3682 can IP CPS1 transiently expressed in COS-7 cells.
Lane 1: 5 ul of 2ml lysate cos-7 cells transfected with full length CPS 1. Before IP
Lane 2: cos-7 cells transfected with full length CPS 1. IP -
Immunocytochemistry/ Immunofluorescence - Anti-CPS1 antibody (ab3682)CPS-1 localisation is consistent with the reticulated pattern of liver mitonchondria.
Rabbit polyclonal to CPS-1 (ab3682), at 1/5000 on COS-7 (African green monkey kidney fibroblast-like cell line) cells transiently transfected with pCMV5-CPS 1 plasmid (Corvi, M.M and Berthiaume, L.G. unpublished data).
Secondary: donkey anti-rabbit (Texas Red).
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Western blot - Anti-CPS1 antibody (ab3682)Western blot using Rabbit polyclonal to CPS 1 (ab3682). All lanes loaded with 100 ng of purified CPS 1
Lane 1: ab3682 1/500
Lane 2: ab3682 1/1000
Lane 3: ab3682 1/2000 Western blot using Rabbit polyclonal to CPS 1 (ab3682).
All lanes loaded with 100 ng of purified CPS 1
Lane 1: ab3682 1/500
Lane 2: ab3682 1/1000
Lane 3: ab3682 1/2000
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (21)
ab3682 has been referenced in 21 publications.
- Kim J et al. The hexosamine biosynthesis pathway is a targetable liability in KRAS/LKB1 mutant lung cancer. Nat Metab 2:1401-1412 (2020). PubMed: 33257855
- Amano H et al. Telomere Dysfunction Induces Sirtuin Repression that Drives Telomere-Dependent Disease. Cell Metab 29:1274-1290.e9 (2019). PubMed: 30930169
- Alsaeedi F et al. Expression of serine/threonine protein kinase SGK1F promotes an hepatoblast state in stem cells directed to differentiate into hepatocytes. PLoS One 14:e0218135 (2019). PubMed: 31242206
- Tata PR et al. Developmental History Provides a Roadmap for the Emergence of Tumor Plasticity. Dev Cell 44:679-693.e5 (2018). PubMed: 29587142
- Wang T et al. L-leucine stimulates glutamate dehydrogenase activity and glutamate synthesis by regulating mTORC1/SIRT4 pathway in pig liver. Anim Nutr 4:329-337 (2018). PubMed: 30175263